dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0006053
Uniprot Accession P15442; GCN2_YEAST; D6VSR3;
Genbank Protein ID NP_010569.3;
Genbank Nucleotide ID NM_001180591.3;
Protein Name Serine/threonine-protein kinase GCN2
Protein Synonyms/Alias
Gene Name GCN2
Gene Synonyms/Alias AAS1;YDR283C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Stimulates GCN4 translation in amino acid-starved cells by phosphorylating the alpha subunit of eIF-2 (SUI2) on 'Ser-52'.
Phosphorylation Sites
 dbPAF PTMs: 19
PositionPeptidesSourceReferences ( PMIDs )
553NSNSLELTPGDTITVdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
567VRGNGGRTLSQSSIRPhosphoGRID 2.021126336
569GNGGRTLSQSSIRRRdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;SysPTM 2.0;curated15665377; 18407956; 16381945; 23193290; 20702584; 19779198;
571GGRTLSQSSIRRRSFcurated25315811
572GRTLSQSSIRRRSFNPhosphoGRID 2.0;PHOSIDA;curated20702584; 19779198; 21081558; 26040289; 22369663; 22817900
577QSSIRRRSFNVGSRFdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated15665377; 18407956; 16381945; 23193290; 21177495; 12070158;
730PDIVFENSSRDDENEPhosphoGRID 2.020489023
759QDDTDKESKSIQNVPPhosphoGRID 2.020489023
761DTDKESKSIQNVPRRdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated17287358; 16381945; 23193290; 21126336; 19779198; 19366988;
877DSQNLPGSSDNLTSAPhosphoGRID 2.020489023
878SQNLPGSSDNLTSAIPhosphoGRID 2.020489023
882PGSSDNLTSAIGTAMdbPTM 3.0;PhosphoGRID 2.0-; 16381945; 23193290; 20404097; 9528799
887NLTSAIGTAMYVATEdbPTM 3.0;PhosphoGRID 2.0-; 16381945; 23193290; 9528799
974NKRPGARTLLNSGWLPhosphoGRID 2.020489023
1002KSLSNPSSPWQQQVRdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
1518FKRWDENSSAGSSQEcurated24961812
1519KRWDENSSAGSSQEGcurated24961812
1522DENSSAGSSQEGDIDcurated24961812
1523ENSSAGSSQEGDIDDcurated24961812; 20377248
Sequence
(Fasta)		MSLSHLTLDQ YYEIQCNELE AIRSIYMDDF TDLTKRKSSW DKQPQIIFEI TLRSVDKEPV 60
ESSITLHFAM TPMYPYTAPE IEFKNVQNVM DSQLQMLKSE FKKIHNTSRG QEIIFEITSF 120
TQEKLDEFQN VVNTQSLEDD RLQRIKETKE QLEKEEREKQ QETIKKRSDE QRRIDEIVQR 180
ELEKRQDDDD DLLFNRTTQL DLQPPSEWVA SGEAIVFSKT IKAKLPNNSM FKFKAVVNPK 240
PIKLTSDIFS FSKQFLVKPY IPPESPLADF LMSSEMMENF YYLLSEIELD NSYFNTSNGK 300
KEIANLEKEL ETVLKAKHDN VNRLFGYTVE RMGRNNATFV WKIRLLTEYC NYYPLGDLIQ 360
SVGFVNLATA RIWMIRLLEG LEAIHKLGIV HKCINLETVI LVKDADFGST IPKLVHSTYG 420
YTVLNMLSRY PNKNGSSVEL SPSTWIAPEL LKFNNAKPQR LTDIWQLGVL FIQIISGSDI 480
VMNFETPQEF LDSTSMDETL YDLLSKMLNN DPKKRLGTLE LLPMKFLRTN IDSTINRFNL 540
VSESVNSNSL ELTPGDTITV RGNGGRTLSQ SSIRRRSFNV GSRFSSINPA TRSRYASDFE 600
EIAVLGQGAF GQVVKARNAL DSRYYAIKKI RHTEEKLSTI LSEVMLLASL NHQYVVRYYA 660
AWLEEDSMDE NVFESTDEES DLSESSSDFE ENDLLDQSSI FKNRTNHDLD NSNWDFISGS 720
GYPDIVFENS SRDDENEDLD HDTSSTSSSE SQDDTDKESK SIQNVPRRRN FVKPMTAVKK 780
KSTLFIQMEY CENRTLYDLI HSENLNQQRD EYWRLFRQIL EALSYIHSQG IIHRDLKPMN 840
IFIDESRNVK IGDFGLAKNV HRSLDILKLD SQNLPGSSDN LTSAIGTAMY VATEVLDGTG 900
HYNEKIDMYS LGIIFFEMIY PFSTGMERVN ILKKLRSVSI EFPPDFDDNK MKVEKKIIRL 960
LIDHDPNKRP GARTLLNSGW LPVKHQDEVI KEALKSLSNP SSPWQQQVRE SLFNQSYSLT 1020
NDILFDNSVP TSTPFANILR SQMTEEVVKI FRKHGGIENN APPRIFPKAP IYGTQNVYEV 1080
LDKGGTVLQL QYDLTYPMAR YLSKNPSLIS KQYRMQHVYR PPDHSRSSLE PRKFGEIDFD 1140
IISKSSSESG FYDAESLKII DEILTVFPVF EKTNTFFILN HADILESVFN FTNIDKAQRP 1200
LVSRMLSQVG FARSFKEVKN ELKAQLNISS TALNDLELFD FRLDFEAAKK RLYKLMIDSP 1260
HLKKIEDSLS HISKVLSYLK PLEVARNVVI SPLSNYNSAF YKGGIMFHAV YDDGSSRNMI 1320
AAGGRYDTLI SFFARPSGKK SSNTRKAVGF NLAWETIFGI AQNYFKLASG NRIKKRNRFL 1380
KDTAVDWKPS RCDVLISSFS NSLLDTIGVT ILNTLWKQNI KADMLRDCSS VDDVVTGAQQ 1440
DGIDWILLIK QQAYPLTNHK RKYKPLKIKK LSTNVDIDLD LDEFLTLYQQ ETGNKSLIND 1500
SLTLGDKADE FKRWDENSSA GSSQEGDIDD VVAGSTNNQK VIYVPNMATR SKKANKREKW 1560
VYEDAARNSS NMILHNLSNA PIITVDALRD ETLEIISITS LAQKEEWLRK VFGSGNNSTP 1620
RSFATSIYNN LSKEAHKGNR WAILYCHKTG KSSVIDLQR
Keyword

KW-0002--3D-structure
KW-0028--Amino-acid biosynthesis
KW-0030--Aminoacyl-tRNA synthetase
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0418--Kinase
KW-0436--Ligase
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
KW-0677--Repeat
KW-0723--Serine/threonine-protein kinase
KW-0346--Stress response
KW-0808--Transferase
Interpro

IPR024435--HisRS-related_dom
IPR011009--Kinase-like_dom
IPR000719--Prot_kinase_dom
IPR017441--Protein_kinase_ATP_BS
IPR006575--RWD-domain
IPR008271--Ser/Thr_kinase_AS
IPR016255--Ser/Thr_kinase_GCN2
IPR016135--UBQ-conjugating_enzyme/RWD
PROSITE

PS00107--PROTEIN_KINASE_ATP
PS50011--PROTEIN_KINASE_DOM
PS00108--PROTEIN_KINASE_ST
PS50908--RWD
Pfam

PF12745--HGTP_anticodon2
PF00069--Pkinase
PF05773--RWD
Gene Ontology

GO:0022626--C:cytosolic ribosome
GO:0015934--C:large ribosomal subunit
GO:0042788--C:polysomal ribosome
GO:0015935--C:small ribosomal subunit
GO:0004812--F:aminoacyl-tRNA ligase activity
GO:0005524--F:ATP binding
GO:0004694--F:eukaryotic translation initiation factor 2alpha kinase activity
GO:0042803--F:protein homodimerization activity
GO:0004672--F:protein kinase activity
GO:0043621--F:protein self-association
GO:0043023--F:ribosomal large subunit binding
GO:0043022--F:ribosome binding
GO:0000049--F:tRNA binding
GO:0008652--P:cellular amino acid biosynthetic process
GO:0034198--P:cellular response to amino acid starvation
GO:0072755--P:cellular response to benomyl
GO:0071232--P:cellular response to histidine
GO:0070301--P:cellular response to hydrogen peroxide
GO:1990451--P:cellular stress response to acidic pH
GO:0001300--P:chronological cell aging
GO:0000077--P:DNA damage checkpoint
GO:0010942--P:positive regulation of cell death
GO:1903833--P:positive regulation of cellular response to amino acid starvation
GO:0071264--P:positive regulation of translational initiation in response to starvation
GO:0046777--P:protein autophosphorylation
GO:0006468--P:protein phosphorylation
GO:0060733--P:regulation of eIF2 alpha phosphorylation by amino acid starvation
GO:0006446--P:regulation of translational initiation
GO:0010998--P:regulation of translational initiation by eIF2 alpha phosphorylation
GO:0006412--P:translation