dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005964
Uniprot Accession P14907; NSP1_YEAST; D6VWE3;
Genbank Protein ID NP_012494.3;
Genbank Nucleotide ID NM_001181474.3;
Protein Name Nucleoporin NSP1
Protein Synonyms/Alias Nuclear pore protein NSP1;Nucleoskeletal-like protein;p110;
Gene Name NSP1
Gene Synonyms/Alias YJL041W;J1207;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NSP1 plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, pre-ribosome, signal recognition particle (SRP), and protein transport.
Phosphorylation Sites
 dbPAF PTMs: 30 (View all)
PositionPeptidesSourceReferences ( PMIDs )
5---MNFNTPQQNKTPcurated26040289
122NGTAGGNTFGSSSLFcurated25315811
127GNTFGSSSLFNNSTNcurated25315811
180NANKPAFSFGATTNDPhosphoGRID 2.021126336
221TTGFSFGSQLGGNKTdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 25315811
238EAAKPSLSFGSGSAGdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19366988; 25315811
273LSFGTATSDNKTTNTcurated25315811
277TATSDNKTTNTTPSFPhosphoGRID 2.021126336
280SDNKTTNTTPSFSFGcurated25315811
281DNKTTNTTPSFSFGAdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22369663; 24961812
283KTTNTTPSFSFGAKSdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22369663; 24961812
285TNTTPSFSFGAKSDEdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22369663; 24961812
290SFSFGAKSDENKAGAcurated24961812
323NSSKPAFSFGAKSNEcurated22817900
361ETSKPAFSFGAKSDEdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 17563356; 19366988; 22369663
366AFSFGAKSDEKKDGDcurated22817900; 24961812; 20377248
375EKKDGDASKPAFSFGdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22817900
456ETSKPAFSFGAKSDEdbPTM 3.0;UniProt17563356; 18407956; 16381945; 23193290
475DASKPAFSFGAKSDEPhosphoGRID 2.019779198
570DGAKAAISFGAKPEEdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
Sequence
(Fasta)		MNFNTPQQNK TPFSFGTANN NSNTTNQNSS TGAGAFGTGQ STFGFNNSAP NNTNNANSSI 60
TPAFGSNNTG NTAFGNSNPT SNVFGSNNST TNTFGSNSAG TSLFGSSSAQ QTKSNGTAGG 120
NTFGSSSLFN NSTNSNTTKP AFGGLNFGGG NNTTPSSTGN ANTSNNLFGA TANANKPAFS 180
FGATTNDDKK TEPDKPAFSF NSSVGNKTDA QAPTTGFSFG SQLGGNKTVN EAAKPSLSFG 240
SGSAGANPAG ASQPEPTTNE PAKPALSFGT ATSDNKTTNT TPSFSFGAKS DENKAGATSK 300
PAFSFGAKPE EKKDDNSSKP AFSFGAKSNE DKQDGTAKPA FSFGAKPAEK NNNETSKPAF 360
SFGAKSDEKK DGDASKPAFS FGAKPDENKA SATSKPAFSF GAKPEEKKDD NSSKPAFSFG 420
AKSNEDKQDG TAKPAFSFGA KPAEKNNNET SKPAFSFGAK SDEKKDGDAS KPAFSFGAKS 480
DEKKDSDSSK PAFSFGTKSN EKKDSGSSKP AFSFGAKPDE KKNDEVSKPA FSFGAKANEK 540
KESDESKSAF SFGSKPTGKE EGDGAKAAIS FGAKPEEQKS SDTSKPAFTF GAQKDNEKKT 600
EESSTGKSTA DVKSSDSLKL NSKPVELKPV SLDNKTLDDL VTKWTNQLTE SASHFEQYTK 660
KINSWDQVLV KGGEQISQLY SDAVMAEHSQ NKIDQSLQYI ERQQDELENF LDNFETKTEA 720
LLSDVVSTSS GAAANNNDQK RQQAYKTAQT LDENLNSLSS NLSSLIVEIN NVSNTFNKTT 780
NIDINNEDEN IQLIKILNSH FDALRSLDDN STSLEKQINS IKK 824
Keyword

KW-0002--3D-structure
KW-0131--Cell cycle
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0472--Membrane
KW-0509--mRNA transport
KW-0906--Nuclear pore complex
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0677--Repeat
KW-0811--Translocation
KW-0813--Transport
Interpro

IPR026010--NSP1/NUP62
IPR007758--Nucleoporin_NSP1_C
PROSITE
Pfam

PF05064--Nsp1_C
Gene Ontology

GO:0031965--C:nuclear membrane
GO:0005643--C:nuclear pore
GO:0044613--C:nuclear pore central transport channel
GO:0044615--C:nuclear pore nuclear basket
GO:0005487--F:nucleocytoplasmic transporter activity
GO:0005543--F:phospholipid binding
GO:0017056--F:structural constituent of nuclear pore
GO:0007049--P:cell cycle
GO:0097064--P:ncRNA export from nucleus
GO:0006607--P:NLS-bearing protein import into nucleus
GO:0016973--P:poly(A)+ mRNA export from nucleus
GO:0006606--P:protein import into nucleus
GO:0000055--P:ribosomal large subunit export from nucleus
GO:0000056--P:ribosomal small subunit export from nucleus
GO:0006409--P:tRNA export from nucleus