dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005805
Uniprot Accession P13712; MSI1_YEAST; D6VQI9;
Genbank Protein ID NP_009754.1;
Genbank Nucleotide ID NM_001178543.1;
Protein Name Chromatin assembly factor 1 subunit p50
Protein Synonyms/Alias
Gene Name MSI1
Gene Synonyms/Alias CAC3;YBR195C;YBR1405;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p50 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in cell extracts (By similarity). Independently, MSI1 is involved in regulation of the RAS/cAMP pathway via sequestration of NPR1.
Phosphorylation Sites
 dbPAF PTMs: 2
PositionPeptidesSourceReferences ( PMIDs )
13KDITHEASSIPIDLQcurated22817900; 24961812
14DITHEASSIPIDLQEdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
Sequence
(Fasta)		MNQCAKDITH EASSIPIDLQ ERYSHWKKNT KLLYDYLNTN STKWPSLTCQ FFPDLDTTSD 60
EHRILLSSFT SSQKPEDETI YISKISTLGH IKWSSLNNFD MDEMEFKPEN STRFPSKHLV 120
NDISIFFPNG ECNRARYLPQ NPDIIAGASS DGAIYIFDRT KHGSTRIRQS KISHPFETKL 180
FGSHGVIQDV EAMDTSSADI NEATSLAWNL QQEALLLSSH SNGQVQVWDI KQYSHENPII 240
DLPLVSINSD GTAVNDVTWM PTHDSLFAAC TEGNAVSLLD LRTKKEKLQS NREKHDGGVN 300
SCRFNYKNSL ILASADSNGR LNLWDIRNMN KSPIATMEHG TSVSTLEWSP NFDTVLATAG 360
QEDGLVKLWD TSCEETIFTH GGHMLGVNDI SWDAHDPWLM CSVANDNSVH IWKPAGNLVG 420
HS 423
Keyword

KW-0114--cAMP
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0677--Repeat
KW-0853--WD repeat
Interpro

IPR022052--Histone-bd_RBBP4_N
IPR015943--WD40/YVTN_repeat-like_dom
IPR001680--WD40_repeat
IPR019775--WD40_repeat_CS
IPR017986--WD40_repeat_dom
PROSITE

PS00678--WD_REPEATS_1
PS50082--WD_REPEATS_2
PS50294--WD_REPEATS_REGION
Pfam

PF12265--CAF1C_H4-bd
PF00400--WD40
Gene Ontology

GO:0033186--C:CAF-1 complex
GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0006335--P:DNA replication-dependent nucleosome assembly