dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005737
Uniprot Accession P13382; DPOA_YEAST; D6W177;
Genbank Protein ID NP_014297.3;
Genbank Nucleotide ID NM_001182940.3;
Protein Name DNA polymerase alpha catalytic subunit A
Protein Synonyms/Alias DNA polymerase I subunit A;DNA polymerase alpha:primase complex p180 subunit;DNA polymerase-primase complex p180 subunit;Pol alpha-primase complex p180 subunit;
Gene Name POL1
Gene Synonyms/Alias CDC17;YNL102W;N2181;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Catalytic component of DNA polymerase alpha, which in complex with DNA primase (DNA polymerase alpha:primase) consitutes a replicative polymerase. POL1 has a role in promoting telomere replication during interaction with CDC13.
Phosphorylation Sites
 dbPAF PTMs: 30 (View all)
PositionPeptidesSourceReferences ( PMIDs )
21LQAARNGTSIDDYEGPhosphoGRID 2.021126336
26NGTSIDDYEGDESDGPhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;curated21177495; 19779198; 21081558; 19060867; 22817900; 19795423
31DDYEGDESDGDRIYDdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 19779198; 21081558;
82DWREVDNSSSDEDTGPhosphoGRID 2.0;PHOSIDA;UniProt;curated20702584; 19779198; 21081558; 26040289; 22817900
83WREVDNSSSDEDTGNPhosphoGRID 2.0;PHOSIDA;UniProt;curated20702584; 19779198; 21081558; 26040289; 22817900
84REVDNSSSDEDTGNLPhosphoGRID 2.0;PHOSIDA;UniProt;curated20702584; 19779198; 21081558; 26040289; 22817900
88NSSSDEDTGNLASKDPhosphoGRID 2.019779198
119DMLRTQHSKSTLLAHcurated19823750; 24961812
121LRTQHSKSTLLAHAKcurated25315811
169KLRENLNSSPTSEFKdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 20702584; 19684113;
170LRENLNSSPTSEFKSdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17287358; 17330950; 17563356; 18407956; 16381945; 23193290;
172ENLNSSPTSEFKSSIdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 17330950; 21177495; 20702584;
173NLNSSPTSEFKSSIKcurated22369663; 22817900; 19547744
188RVNGNDESSHDAGISPhosphoPep 2.0;curated19060867; 26040289; 24961812; 25315811
189VNGNDESSHDAGISKPhosphoPep 2.0;curated19060867; 26040289; 24961812; 25315811
204KVKIDPDSSTDKYLEdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;curated17563356; 16381945; 23193290; 19823750; 19795423; 21081558;
205VKIDPDSSTDKYLEIdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19823750; 19779198; 19795423;
206KIDPDSSTDKYLEIEPhosphoGRID 2.0;PhosphoPep 2.0;curated19823750; 19779198; 19795423; 19060867; 22817900; 25882841;
209PDSSTDKYLEIESSPdbPTM 3.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22817900; 19823750;
214DKYLEIESSPLKLQSdbPTM 3.0;PHOSIDA;SysPTM 2.0;curated18407956; 16381945; 23193290; 21081558; 19366988; 22369663;
Sequence
(Fasta)		MSSKSEKLEK LRKLQAARNG TSIDDYEGDE SDGDRIYDEI DEKEYRARKR QELLHDDFVV 60
DDDGVGYVDR GVEEDWREVD NSSSDEDTGN LASKDSKRKK NIKREKDHQI TDMLRTQHSK 120
STLLAHAKKS QKKSIPIDNF DDILGEFESG EVEKPNILLP SKLRENLNSS PTSEFKSSIK 180
RVNGNDESSH DAGISKKVKI DPDSSTDKYL EIESSPLKLQ SRKLRYANDV QDLLDDVENS 240
PVVATKRQNV LQDTLLANPP SAQSLADEED DEDSDEDIIL KRRTMRSVTT TRRVNIDSRS 300
NPSTSPFVTA PGTPIGIKGL TPSKSLQSNT DVATLAVNVK KEDVVDPETD TFQMFWLDYC 360
EVNNTLILFG KVKLKDDNCV SAMVQINGLC RELFFLPREG KTPTDIHEEI IPLLMDKYGL 420
DNIRAKPQKM KYSFELPDIP SESDYLKVLL PYQTPKSSRD TIPSDLSSDT FYHVFGGNSN 480
IFESFVIQNR IMGPCWLDIK GADFNSIRNA SHCAVEVSVD KPQNITPTTT KTMPNLRCLS 540
LSIQTLMNPK ENKQEIVSIT LSAYRNISLD SPIPENIKPD DLCTLVRPPQ STSFPLGLAA 600
LAKQKLPGRV RLFNNEKAML SCFCAMLKVE DPDVIIGHRL QNVYLDVLAH RMHDLNIPTF 660
SSIGRRLRRT WPEKFGRGNS NMNHFFISDI CSGRLICDIA NEMGQSLTPK CQSWDLSEMY 720
QVTCEKEHKP LDIDYQNPQY QNDVNSMTMA LQENITNCMI SAEVSYRIQL LTLTKQLTNL 780
AGNAWAQTLG GTRAGRNEYI LLHEFSRNGF IVPDKEGNRS RAQKQRQNEE NADAPVNSKK 840
AKYQGGLVFE PEKGLHKNYV LVMDFNSLYP SIIQEFNICF TTVDRNKEDI DELPSVPPSE 900
VDQGVLPRLL ANLVDRRREV KKVMKTETDP HKRVQCDIRQ QALKLTANSM YGCLGYVNSR 960
FYAKPLAMLV TNKGREILMN TRQLAESMNL LVVYGDTDSV MIDTGCDNYA DAIKIGLGFK 1020
RLVNERYRLL EIDIDNVFKK LLLHAKKKYA ALTVNLDKNG NGTTVLEVKG LDMKRREFCP 1080
LSRDVSIHVL NTILSDKDPE EALQEVYDYL EDIRIKVETN NIRIDKYKIN MKLSKDPKAY 1140
PGGKNMPAVQ VALRMRKAGR VVKAGSVITF VITKQDEIDN AADTPALSVA ERAHALNEVM 1200
IKSNNLIPDP QYYLEKQIFA PVERLLERID SFNVVRLSEA LGLDSKKYFR REGGNNNGED 1260
INNLQPLETT ITDVERFKDT VTLELSCPSC DKRFPFGGIV SSNYYRVSYN GLQCKHCEQL 1320
FTPLQLTSQI EHSIRAHISL YYAGWLQCDD STCGIVTRQV SVFGKRCLND GCTGVMRYKY 1380
SDKQLYNQLL YFDSLFDCEK NKKQELKPIY LPDDLDYPKE QLTESSIKAL TEQNRELMET 1440
GRSVVQKYLN DCGRRYVDMT SIFDFMLN 1469
Keyword

KW-0002--3D-structure
KW-0004--4Fe-4S
KW-0007--Acetylation
KW-0181--Complete proteome
KW-0235--DNA replication
KW-0238--DNA-binding
KW-0239--DNA-directed DNA polymerase
KW-0408--Iron
KW-0411--Iron-sulfur
KW-0479--Metal-binding
KW-0548--Nucleotidyltransferase
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0808--Transferase
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR006172--DNA-dir_DNA_pol_B
IPR017964--DNA-dir_DNA_pol_B_CS
IPR006133--DNA-dir_DNA_pol_B_exonuc
IPR006134--DNA-dir_DNA_pol_B_multi_dom
IPR024647--DNA_pol_a_cat_su_N
IPR023211--DNA_pol_palm_dom
IPR029702--POLA/Pol1
IPR012337--RNaseH-like_dom
IPR015088--Znf_DNA-dir_DNA_pol_B_alpha
PROSITE

PS00116--DNA_POLYMERASE_B
Pfam

PF12254--DNA_pol_alpha_N
PF00136--DNA_pol_B
PF03104--DNA_pol_B_exo1
PF08996--zf-DNA_Pol
Gene Ontology

GO:0005658--C:alpha DNA polymerase:primase complex
GO:0005657--C:replication fork
GO:0008408--F:3'-5' exonuclease activity
GO:0051539--F:4 iron, 4 sulfur cluster binding
GO:0003677--F:DNA binding
GO:0003887--F:DNA-directed DNA polymerase activity
GO:0046872--F:metal ion binding
GO:0001882--F:nucleoside binding
GO:0000166--F:nucleotide binding
GO:0006260--P:DNA replication
GO:0006270--P:DNA replication initiation
GO:0000731--P:DNA synthesis involved in DNA repair
GO:0006302--P:double-strand break repair
GO:0006303--P:double-strand break repair via nonhomologous end joining
GO:0006273--P:lagging strand elongation
GO:0006272--P:leading strand elongation
GO:0006279--P:premeiotic DNA replication
GO:0006278--P:RNA-dependent DNA replication