dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005691
Uniprot Accession P12962; CAF20_YEAST; D6W2X6;
Genbank Protein ID NP_014919.3;
Genbank Nucleotide ID NM_001183695.3;
Protein Name Cap-associated protein CAF20
Protein Synonyms/Alias 20 kDa cap-associated protein;CCR4-associated factor 2;p20;
Gene Name CAF20
Gene Synonyms/Alias CAF2;CAP20;YOR276W;O5453W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Acts as an inhibitor of cap-dependent translation. Competes with eIF4G1/TIF4631 and EAP1 for binding to eIF4E/TIF45 and interferes with the formation of the eIF4F complex, inhibiting translation and stabilizing mRNA. Binding affinity for eIF4E/TIF45 is 10-fold less than that of eIF4G1/TIF4631. Required for induction of pseudohyphal growth in response to nitrogen limitation, probably by regulating STE12 translation.
Phosphorylation Sites
 dbPAF PTMs: 16
PositionPeptidesSourceReferences ( PMIDs )
58GHFGRRRSSHHHGRPPhosphoGRID 2.0;curated12372297; 24961812
59HFGRRRSSHHHGRPKPhosphoGRID 2.0;curated12372297; 24961812
75KHNKPKVTTDSDGWCPhosphoPep 2.0;curated19060867; 19547744
76HNKPKVTTDSDGWCTPhosphoPep 2.019060867
78KPKVTTDSDGWCTFEPhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated21177495; 19684113; 19823750; 19779198; 19795423; 19060867;
91FEAKKKGSGEDDEEEdbPTM 3.0;Phospho.ELM 9.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 17563356; 18407956; 16381945; 23193290; 7592868; 2
99GEDDEEETETTPTSTdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 17330950; 21177495;
101DDEEETETTPTSTVPdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 21177495; 20702584; 19823750;
102DEEETETTPTSTVPVdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15665377; 17330950; 17563356; 18407956; 16381945; 23193290;
104EETETTPTSTVPVATdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 21177495; 19779198; 19060867;
105ETETTPTSTVPVATIdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 21177495; 19060867; 19366988;
106TETTPTSTVPVATIAdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 21177495; 19779198; 19060867;
111TSTVPVATIAQETLKcurated24961812; 25521595
127KPNNKNISSNRPADTcurated19823750; 25315811
134SSNRPADTRDIVADKcurated19823750
154NAFAALESEDEDDEAdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19684113; 19823750; 19779198;
Sequence
(Fasta)		MIKYTIDELF QLKPSLTLEV NFDAVEFRAI IEKVKQLQHL KEEEFNSHHV GHFGRRRSSH 60
HHGRPKIKHN KPKVTTDSDG WCTFEAKKKG SGEDDEEETE TTPTSTVPVA TIAQETLKVK 120
PNNKNISSNR PADTRDIVAD KPILGFNAFA ALESEDEDDE A 162
Keyword

KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0396--Initiation factor
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-0652--Protein synthesis inhibitor
KW-1185--Reference proteome
KW-0810--Translation regulation
Interpro

IPR031456--Caf20
PROSITE
Pfam

PF17052--CAF20
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005845--C:mRNA cap binding complex
GO:0003743--F:translation initiation factor activity
GO:0017148--P:negative regulation of translation
GO:0010606--P:positive regulation of cytoplasmic mRNA processing body assembly