dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005686
Uniprot Accession P12954; SRS2_YEAST; D6VW92;
Genbank Protein ID NP_012443.1;
Genbank Nucleotide ID NM_001181525.1;
Protein Name ATP-dependent DNA helicase SRS2
Protein Synonyms/Alias
Gene Name SRS2
Gene Synonyms/Alias HPR5;RADH;YJL092W;J0913;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 ATP-dependent DNA helicase involved in DNA repair at least for UV-induced lesions. The polarity of the helicase activity was determined to be 3' to 5'.
Phosphorylation Sites
 dbPAF PTMs: 15
PositionPeptidesSourceReferences ( PMIDs )
481FQILKDISSKKIMLDcurated22817900
604PIENKVITPKEYLRNPhosphoGRID 2.020195513; 20713444; 15964827
682NDDKKDESEEDEEEDcurated26040289; 25315811
698ENSKKDASPKKTRVLPhosphoGRID 2.020195513; 20713444; 15964827
833SRNKLLGSVSDFTSAdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19366988
879APKSRVKSPEKRYAPPhosphoGRID 2.020195513; 20713444; 15964827; 19520826
893PETTSFHSPTKKKVYdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17287358; 16381945; 23193290; 20195513; 20713444; 15964827;
904KKVYAPQYVSTTNVPcurated26040289
906VYAPQYVSTTNVPSRcurated26040289
907YAPQYVSTTNVPSRQcurated26040289
933FLRREDRSITDISPRdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 25315811
935RREDRSITDISPRSScurated22817900
938DRSITDISPRSSTRSPhosphoGRID 2.0;curated20195513; 20713444; 15964827; 22369663; 22817900; 26040289;
950TRSLKGASPNKTSHMPhosphoGRID 2.020195513; 20713444; 15964827
965SDDLMRPSPTRKDKVPhosphoGRID 2.0;curated20195513; 20713444; 15964827; 24961812
Sequence
(Fasta)		MSSNNDLWLH LVSQLNTQQR AAALFDYTRG LQVIAGPGTG KTKVLTSRVA YLILHHHIHP 60
RDIIVTTFTN KAANEMKERL QEMLRGAGVN ISELLIGTFH SICLKILYRF GHLVDLQKDW 120
RIIDEKEIDV ILDDMIEKVP DQIRDYASSI TRKVNLCMPS KNGDEWTIHP KLIKKQISKL 180
KSNAILPEEY ILDSNHDAAL GYFYQIYQSE LSKKNTLDFD DLLMYTFRLL TRVRVLSNIK 240
HVLVDEFQDT NGIQLDLMFL FAKGNHHLSR GMTIVGDPDQ SIYAFRNALA HNFLEMGRKC 300
PIEYSTIILV ENYRSSQKIL NTSEILITQQ NKGRQNRAPL RAQFDLDFPP VYMNFPAYFL 360
EAPSLVRELL YLKALPNLFT FNDFAILVRQ RRQIKRIESA LIEHRIPYKI IRGHSFWDSK 420
ETRAMLNLLK LIFSPNDKHA ILASLLYPAR GLGPATGEKI KNALDTLATD VSCFQILKDI 480
SSKKIMLDIP TKGRSVIADF ISMIENCQLL LQSTLLGGLS DLFDKLYELS GLKYEYLYKD 540
GKKKNDQLEK SEPNLLNARH KNIELLKNYF LALLSKSESS DKEKNEAIKA ATDEAEPIEN 600
KVITPKEYLR NFFNSLSLHS DAAEEEESES NKDAKIKREK NGFVTISTIH GAKGLEWPVV 660
FIPGCEEGII PCVFNDDKKD ESEEDEEEDQ ENSKKDASPK KTRVLSVEDS IDEERRMFFV 720
AQTRAKYLLY LSNTVTVEDV DRPRIASRFL TTDLIKAMSD SQKLFESTNS IKKLYRILNK 780
KPPAEDDKLF SLDQLRKDYN QFIENRRERM IWQGIQMNDV YGIQLSRNKL LGSVSDFTSA 840
ADQLRLETQN SIFPQKKLIE KSRPSKINGN YAPKSRVKSP EKRYAPETTS FHSPTKKKVY 900
APQYVSTTNV PSRQEFHSST GKNIPFLRRE DRSITDISPR SSTRSLKGAS PNKTSHMSDD 960
LMRPSPTRKD KVTRNIHFAT AGTFRIETQS NVDELHPPEY SNKSGQSLTS SEFSGFSSAC 1020
SNSDQPTNLI EDINNELDLS DEELLNDISI ERRRELLGSK KTKKIKPKTR NRKSKRGDKV 1080
KVEEVIDLKS EFEEDDSRNT TAAELLHNPD DTTVDNRPII SNAKFLADAA MKKTQKFSKK 1140
VKNEPASSQM DIFSQLSRAK KKSKLNNGEI IVID 1175
Keyword

KW-0002--3D-structure
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0238--DNA-binding
KW-0347--Helicase
KW-0378--Hydrolase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
Interpro

IPR013986--DExx_box_DNA_helicase_dom
IPR014017--DNA_helicase_UvrD-like_C
IPR000212--DNA_helicase_UvrD/REP
IPR027417--P-loop_NTPase
IPR014016--UvrD-like_ATP-bd
PROSITE

PS51198--UVRD_HELICASE_ATP_BIND
PS51217--UVRD_HELICASE_CTER
Pfam

PF00580--UvrD-helicase
PF13361--UvrD_C
Gene Ontology

GO:0005634--C:nucleus
GO:0005524--F:ATP binding
GO:0004003--F:ATP-dependent DNA helicase activity
GO:0003677--F:DNA binding
GO:0003678--F:DNA helicase activity
GO:0008047--F:enzyme activator activity
GO:0032508--P:DNA duplex unwinding
GO:0006281--P:DNA repair
GO:0006303--P:double-strand break repair via nonhomologous end joining
GO:0045910--P:negative regulation of DNA recombination
GO:2000042--P:negative regulation of double-strand break repair via homologous recombination
GO:0032079--P:positive regulation of endodeoxyribonuclease activity
GO:0032986--P:protein-DNA complex disassembly