| Tag |
Content |
| dbPAF ID |
dbPAF-0005675 |
| Uniprot Accession |
P12868; PEP5_YEAST; D6W057;
|
| Genbank Protein ID |
NP_013958.1;
|
| Genbank Nucleotide ID |
NM_001182738.1;
|
| Protein Name |
E3 ubiquitin-protein ligase PEP5 |
| Protein Synonyms/Alias |
Carboxypeptidase Y-deficient protein 5;Histone E3 ligase PEP5;Vacuolar biogenesis protein END1;Vacuolar morphogenesis protein 1;Vacuolar protein sorting-associated protein 11;Vacuolar protein-targeting protein 11; |
| Gene Name |
PEP5 |
| Gene Synonyms/Alias |
END1;VAM1;VPL9;VPS11;VPT11;YMR231W;YM9959.13; |
| Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast) |
| NCBI Taxa ID |
559292 |
Functional Description
(View all) |
Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. It is required for gluconeogenic growth of yeast. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion (PubMed:3062374, PubMed:10978279, PubMed:10944212, PubMed:16601699). Acts as component of the CORVET complex that is required for transport between endosome and vacuole. CORVET is an effector for the endosomal Rab GTPase VPS21 (PubMed:17488625). Probable ubiquitin-protein ligase involved in the degradation-related ubiquitination of histones. Contributes to the post-translational regulation of histone protein levels by polyubiquitination of excess histones for subsequent degradation (PubMed:22570702).Functional Description
Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. It is required for gluconeogenic growth of yeast. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion (PubMed:3062374, PubMed:10978279, PubMed:10944212, PubMed:16601699). Acts as component of the CORVET complex that is required for transport between endosome and vacuole. CORVET is an effector for the endosomal Rab GTPase VPS21 (PubMed:17488625). Probable ubiquitin-protein ligase involved in the degradation-related ubiquitination of histones. Contributes to the post-translational regulation of histone protein levels by polyubiquitination of excess histones for subsequent degradation (PubMed:22570702). |
Phosphorylation Sites
|
dbPAF PTMs: 4 |
Sequence
(Fasta) | MSLSSWRQFQ LFENIPIRDP NFGGDSLLYS DPTLCAATIV DPQTLIIAVN SNIIKVVKLN 60
QSQVIHEFQS FPHDFQITFL KVINGEFLVA LAESIGKPSL IRVYKLEKLP NREQLYHSQV 120
ELKNGNNTYP ISVVSISNDL SCIVVGFING KIILIRGDIS RDRGSQQRII YEDPSKEPIT 180
ALFLNNDATA CFAATTSRIL LFNTTGRNRG RPSLVLNSKN GLDLNCGSFN PATNEFICCL 240
SNFIEFFSSS GKKHQFAFDL SLRKRIFCVD KDHILIVTEE TGVPTTSISV NELSPTIINR 300
IFIIDAKNKI ISLNFVVSSA IIDIFSTSQS GKNITYLLTS EGVMHRITPK SLENQINIII 360
QKELYPFALQ LAKQHSLSPL DVQEIHKKYG DYLFKKGLRK EATDQYIQCL DVVETSEIIS 420
KFGVKEVPDP ESMRNLADYL WSLIKNSISQ RDHVTLLLIV LIKLKDVEGI DTFIQHFDRK 480
GIWNEGVVMD DMDDVTFFYS DNDFFDLDLI LELMKESDFK RLSYRLAKKY SKDSLIIVDI 540
LLNLLHNPVK AIKYIKSLPI DETLRCLVTY SKKLLEESPN ETNALLIEVF TGKFKPSTFE 600
VDLDRRDTTG DFSENIRTVF YSYKTFFNYM NSNGTSDAMS ESSEASHEHE EPTYHPPKPS 660
IVFSSFVTKP FEFVVFLEAC LACYQQYEGF DEDRQVILTT LYDLYLNLAQ NDVPERIDDW 720
RSRATGVLRE SNKLVYSAAS NNTSKRVDNS IMLLISHMDQ SSASAKDKTK IDIASFANDN 780
PEMDLLSTFR AMTLNEEPST CLKFLEKYGT EEPKLLQVAL SYFVSNKLIF KEMGGNEVLK 840
EKVLRPIIEG ERMPLLDIIK ALSRTNVAHF GLIQDIIIDH VKTEDTEIKR NEKLIESYDK 900
ELKEKNKKLK NTINSDQPLH VPLKNQTCFM CRLTLDIPVV FFKCGHIYHQ HCLNEEEDTL 960
ESERKLFKCP KCLVDLETSN KLFEAQHEVV EKNDLLNFAL NSEEGSRDRF KVITEFLGRG 1020
AISYSDITI
Fasta Sequence
>P12868|PEP5|Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
MSLSSWRQFQLFENIPIRDPNFGGDSLLYSDPTLCAATIVDPQTLIIAVNSNIIKVVKLNQSQVIHEFQSFPHDFQITFLKVINGEFLVALAESIGKPSLIRVYKLEKLPNREQLYHSQVELKNGNNTYPISVVSISNDLSCIVVGFINGKIILIRGDISRDRGSQQRIIYEDPSKEPITALFLNNDATACFAATTSRILLFNTTGRNRGRPSLVLNSKNGLDLNCGSFNPATNEFICCLSNFIEFFSSSGKKHQFAFDLSLRKRIFCVDKDHILIVTEETGVPTTSISVNELSPTIINRIFIIDAKNKIISLNFVVSSAIIDIFSTSQSGKNITYLLTSEGVMHRITPKSLENQINIIIQKELYPFALQLAKQHSLSPLDVQEIHKKYGDYLFKKGLRKEATDQYIQCLDVVETSEIISKFGVKEVPDPESMRNLADYLWSLIKNSISQRDHVTLLLIVLIKLKDVEGIDTFIQHFDRKGIWNEGVVMDDMDDVTFFYSDNDFFDLDLILELMKESDFKRLSYRLAKKYSKDSLIIVDILLNLLHNPVKAIKYIKSLPIDETLRCLVTYSKKLLEESPNETNALLIEVFTGKFKPSTFEVDLDRRDTTGDFSENIRTVFYSYKTFFNYMNSNGTSDAMSESSEASHEHEEPTYHPPKPSIVFSSFVTKPFEFVVFLEACLACYQQYEGFDEDRQVILTTLYDLYLNLAQNDVPERIDDWRSRATGVLRESNKLVYSAASNNTSKRVDNSIMLLISHMDQSSASAKDKTKIDIASFANDNPEMDLLSTFRAMTLNEEPSTCLKFLEKYGTEEPKLLQVALSYFVSNKLIFKEMGGNEVLKEKVLRPIIEGERMPLLDIIKALSRTNVAHFGLIQDIIIDHVKTEDTEIKRNEKLIESYDKELKEKNKKLKNTINSDQPLHVPLKNQTCFMCRLTLDIPVVFFKCGHIYHQHCLNEEEDTLESERKLFKCPKCLVDLETSNKLFEAQHEVVEKNDLLNFALNSEEGSRDRFKVITEFLGRGAISYSDITI
|
| Keyword |
KW-0181--Complete proteome
KW-0436--Ligase
KW-0472--Membrane
KW-0479--Metal-binding
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0813--Transport
KW-0833--Ubl conjugation pathway
KW-0926--Vacuole
KW-0862--Zinc
KW-0863--Zinc-finger
|
| Interpro |
IPR000547--Clathrin_H-chain/VPS_repeat
IPR016528--VPS11
IPR024763--VPS11_C
IPR015943--WD40/YVTN_repeat-like_dom
IPR017986--WD40_repeat_dom
IPR001965--Znf_PHD
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
|
| PROSITE |
PS50236--CHCR
PS50089--ZF_RING_2
|
| Pfam |
PF12451--VPS11_C
|
| Gene Ontology |
GO:0033263--C:CORVET complex
GO:0000329--C:fungal-type vacuole membrane
GO:0030897--C:HOPS complex
GO:0016874--F:ligase activity
GO:0030674--F:protein binding, bridging
GO:0004842--F:ubiquitin-protein transferase activity
GO:0008270--F:zinc ion binding
GO:0007032--P:endosome organization
GO:0006895--P:Golgi to endosome transport
GO:0036205--P:histone catabolic process
GO:0016574--P:histone ubiquitination
GO:0006886--P:intracellular protein transport
GO:0045324--P:late endosome to vacuole transport
GO:0007040--P:lysosome organization
GO:0035542--P:regulation of SNARE complex assembly
GO:0032889--P:regulation of vacuole fusion, non-autophagic
GO:0042144--P:vacuole fusion, non-autophagic
GO:0006904--P:vesicle docking involved in exocytosis
|
