| Tag |
Content |
| dbPAF ID |
dbPAF-0005656 |
| Uniprot Accession |
P12754; EI2BD_YEAST; D6VUL5;
|
| Genbank Protein ID |
NP_011597.1;
|
| Genbank Nucleotide ID |
NM_001181212.1;
|
| Protein Name |
Translation initiation factor eIF-2B subunit delta |
| Protein Synonyms/Alias |
GCD complex subunit GCD2;Guanine nucleotide exchange factor subunit GCD2;eIF-2B GDP-GTP exchange factor subunit delta; |
| Gene Name |
GCD2 |
| Gene Synonyms/Alias |
TIF224;YGR083C; |
| Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast) |
| NCBI Taxa ID |
559292 |
Functional Description
(View all) |
Acts as essential component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD2 is also required for cell viability. Its function can partially be replaced by GCN3 under normal growth conditions in GCD2-defective mutants, under AA starvation conditions GCN3 is an antagonist (GCN4 translational activator).Functional Description
Acts as essential component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD2 is also required for cell viability. Its function can partially be replaced by GCN3 under normal growth conditions in GCD2-defective mutants, under AA starvation conditions GCN3 is an antagonist (GCN4 translational activator). |
Phosphorylation Sites
|
dbPAF PTMs: 8 |
Sequence
(Fasta) | MSESEAKSRS ATPPSKAKQA TPTTTAAANG EKKLTNKELK ELKKQEKAAK RAAMKQANGI 60
SIEQQQQQAQ MKKEKKQLQR EQQQKREQKQ KNANKKKQNE RNVKKSTLFG HLETTEERRA 120
TILALTSAVS SPKTSRITAA GLMVPVVASA LSGSNVLTAS SLMPVGPNAS STVSASAPAS 180
TTTTLPASSA ALSAGTSSAS TNTPTAIQQE IASSNASDVA KTLASISLEA GEFNVIPGIS 240
SVIPTVLEQS FDNSSLISSV KELLLNKDLI HPSILLLTSH LAHYKIVGSI PRCIAMLEVF 300
QIVIKDYQTP KGTTLSRNLT SYLSHQIDLL KKARPLSVTM GNAIRWLKQE ISLIDPSTPD 360
KAAKKDLCEK IGQFAKEKIE LADQLIIDNA STQIEESTTI VTYGSSKVLT ELLLHNAISL 420
KKNIKVIVVD SRPLFEGRKM AETLRNAGVN VMYALITSLD TIFNMDVDYV FLGAHSILSN 480
GFLYSRAGTA MLAMSAKRRN IPVLVCCESL KFSQRVQLDS VTFNELADPN DLVNIDYENP 540
VERRGNKGAL LNQFIKERKF EKKKLAMENK PKGNKIGGKK GSEGESKDAS NEEDSNSKNI 600
LDGWQELPSL NIVNILYDLT PPEYIKKVIT EFGALPPSSV PVILREYKGS A
652Fasta Sequence
>P12754|GCD2|Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
MSESEAKSRSATPPSKAKQATPTTTAAANGEKKLTNKELKELKKQEKAAKRAAMKQANGISIEQQQQQAQMKKEKKQLQREQQQKREQKQKNANKKKQNERNVKKSTLFGHLETTEERRATILALTSAVSSPKTSRITAAGLMVPVVASALSGSNVLTASSLMPVGPNASSTVSASAPASTTTTLPASSAALSAGTSSASTNTPTAIQQEIASSNASDVAKTLASISLEAGEFNVIPGISSVIPTVLEQSFDNSSLISSVKELLLNKDLIHPSILLLTSHLAHYKIVGSIPRCIAMLEVFQIVIKDYQTPKGTTLSRNLTSYLSHQIDLLKKARPLSVTMGNAIRWLKQEISLIDPSTPDKAAKKDLCEKIGQFAKEKIELADQLIIDNASTQIEESTTIVTYGSSKVLTELLLHNAISLKKNIKVIVVDSRPLFEGRKMAETLRNAGVNVMYALITSLDTIFNMDVDYVFLGAHSILSNGFLYSRAGTAMLAMSAKRRNIPVLVCCESLKFSQRVQLDSVTFNELADPNDLVNIDYENPVERRGNKGALLNQFIKERKFEKKKLAMENKPKGNKIGGKKGSEGESKDASNEEDSNSKNILDGWQELPSLNIVNILYDLTPPEYIKKVITEFGALPPSSVPVILREYKGSA
|
| Keyword |
KW-0007--Acetylation
KW-0181--Complete proteome
KW-0396--Initiation factor
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
KW-0678--Repressor
KW-0810--Translation regulation
|
| Interpro |
IPR000649--IF-2B-related
|
| PROSITE |
|
| Pfam |
PF01008--IF-2B
|
| Gene Ontology |
GO:0005737--C:cytoplasm
GO:0005851--C:eukaryotic translation initiation factor 2B complex
GO:0032045--C:guanyl-nucleotide exchange factor complex
GO:0030234--F:enzyme regulator activity
GO:0003743--F:translation initiation factor activity
GO:0045947--P:negative regulation of translational initiation
GO:0043547--P:positive regulation of GTPase activity
GO:0050790--P:regulation of catalytic activity
GO:0006446--P:regulation of translational initiation
|
