dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005647
Uniprot Accession P12688; YPK1_YEAST; D6VX69;
Genbank Protein ID NP_012796.1;
Genbank Nucleotide ID NM_001179692.1;
Protein Name Serine/threonine-protein kinase YPK1
Protein Synonyms/Alias Sphingosine-like immunosuppressant resistant protein 2;Yeast protein kinase 1;
Gene Name YPK1
Gene Synonyms/Alias SLI2;YKL126W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Plays an essential role in the proliferation of yeast cells. Involved in a signaling pathway, required for optimal cell wall integrity, that acts in parallel with the PKC1-SLT2-dependent pathway. Downstream kinase in the sphingolipid-mediated signaling pathway. Its phosphorylation is regulated by the intracellular sphingolipid concentration. Cooperates with SLI1 in mediating resistance to the sphingolipid biosynthesis inhibitor drug myriocin (ISP-1). Its kinase activity is essential for the resistance. Required for both receptor-mediated and fluid-phase endocytosis, but is not necessary for receptor phosphorylation or ubiquitination. Necessary for the internalization of plasma membrane proteins carrying different types of internalization signals. Acts downstream of the PKH kinases to control endocytosis by phosphorylating components of the endocytic machinery. Phosphorylation of residue Thr-504 in the activation loop is essential for activity. phosphorylates and down-regulates flippase activator FPK1.
Phosphorylation Sites
 dbPAF PTMs: 33 (View all)
PositionPeptidesSourceReferences ( PMIDs )
22EEKEAKHSGFFHSSKcurated22817900; 19547744
39EQQNNQATAGEHDAScurated19823750
46TAGEHDASITRSSLDPhosphoPep 2.0;curated19060867; 25492886; 19823750; 24961812; 25315811; 20377248
48GEHDASITRSSLDRKdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 21177495; 19060867; 19366988;
50HDASITRSSLDRKGTdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17287358; 16381945; 23193290; 17330950; 19060867; 19366988;
51DASITRSSLDRKGTIdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17287358; 17563356; 16381945; 23193290; 17330950; 21177495;
57SSLDRKGTINPSNSSdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 20489023; 19779198;
61RKGTINPSNSSVVPVdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 20489023; 19779198; 19060867;
63GTINPSNSSVVPVRVPhosphoGRID 2.0;PhosphoPep 2.0;curated20489023; 19060867; 25315811
64TINPSNSSVVPVRVSPhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated20489023; 19779198; 19060867; 26040289; 22817900
71SVVPVRVSYDASSSTdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 17330950; 20489023;
72VVPVRVSYDASSSTSdbPTM 3.0;PhosphoPep 2.0;SysPTM 2.018407956; 16381945; 23193290; 19060867; 19366988
75VRVSYDASSSTSTVRdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17563356; 16381945; 23193290; 17330950; 19779198; 19366988;
76RVSYDASSSTSTVRDPhosphoGRID 2.0;curated19779198; 22369663; 22817900; 26040289
77VSYDASSSTSTVRDSdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19779198; 19366988; 26040289;
78SYDASSSTSTVRDSNPhosphoGRID 2.0;PhosphoPep 2.0;curated19779198; 19060867; 26040289; 25315811
79YDASSSTSTVRDSNGPhosphoPep 2.0;curated19060867; 26040289; 25315811
80DASSSTSTVRDSNGGPhosphoGRID 2.0;PHOSIDA;curated19779198; 21081558; 26040289; 22817900
159PPPHKEISKEVDALIdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
170DALIAQLSRVQIKNQdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19366988; 22817900
Sequence
(Fasta)		MYSWKSKFKF GKSKEEKEAK HSGFFHSSKK EEQQNNQATA GEHDASITRS SLDRKGTINP 60
SNSSVVPVRV SYDASSSTST VRDSNGGNSE NTNSSQNLDE TANIGSTGTP NDATSSSGMM 120
TIKVYNGDDF ILPFPITSSE QILNKLLASG VPPPHKEISK EVDALIAQLS RVQIKNQGPA 180
DEDLISSESA AKFIPSTIML PGSSTLNPLL YFTIEFDNTV ATIEAEYGTI AKPGFNKIST 240
FDVTRKLPYL KIDVFARIPS ILLPSKTWQQ EMGLQDEKLQ TIFDKINSNQ DIHLDSFHLP 300
INLSFDSAAS IRLYNHHWIT LDNGLGKINI SIDYKPSRNK PLSIDDFDLL KVIGKGSFGK 360
VMQVRKKDTQ KVYALKAIRK SYIVSKSEVT HTLAERTVLA RVDCPFIVPL KFSFQSPEKL 420
YFVLAFINGG ELFYHLQKEG RFDLSRARFY TAELLCALDN LHKLDVVYRD LKPENILLDY 480
QGHIALCDFG LCKLNMKDDD KTDTFCGTPE YLAPELLLGL GYTKAVDWWT LGVLLYEMLT 540
GLPPYYDEDV PKMYKKILQE PLVFPDGFDR DAKDLLIGLL SRDPTRRLGY NGADEIRNHP 600
FFSQLSWKRL LMKGYIPPYK PAVSNSMDTS NFDEEFTREK PIDSVVDEYL SESVQKQFGG 660
WTYVGNEQLG SSMVQGRSIR 681
Keyword

KW-0067--ATP-binding
KW-1003--Cell membrane
KW-0961--Cell wall biogenesis/degradation
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0254--Endocytosis
KW-0418--Kinase
KW-0472--Membrane
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0808--Transferase
Interpro

IPR000961--AGC-kinase_C
IPR011009--Kinase-like_dom
IPR017892--Pkinase_C
IPR000719--Prot_kinase_dom
IPR017441--Protein_kinase_ATP_BS
IPR002290--Ser/Thr_dual-sp_kinase
IPR008271--Ser/Thr_kinase_AS
PROSITE

PS51285--AGC_KINASE_CTER
PS00107--PROTEIN_KINASE_ATP
PS50011--PROTEIN_KINASE_DOM
PS00108--PROTEIN_KINASE_ST
Pfam

PF00069--Pkinase
PF00433--Pkinase_C
Gene Ontology

GO:0005935--C:cellular bud neck
GO:0005829--C:cytosol
GO:0005886--C:plasma membrane
GO:0005524--F:ATP binding
GO:0019887--F:protein kinase regulator activity
GO:0004674--F:protein serine/threonine kinase activity
GO:0071555--P:cell wall organization
GO:0070941--P:eisosome assembly
GO:0006897--P:endocytosis
GO:0006650--P:glycerophospholipid metabolic process
GO:0061093--P:negative regulation of phospholipid translocation
GO:0090157--P:negative regulation of sphingolipid biosynthesis involved in cellular sphingolipid homeostasis
GO:0046777--P:protein autophosphorylation
GO:0006468--P:protein phosphorylation
GO:0045859--P:regulation of protein kinase activity
GO:0000749--P:response to pheromone involved in conjugation with cellular fusion