dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005408
Uniprot Accession P10862; RAD18_YEAST; D6VR69; Q58AT6;
Genbank Protein ID NP_009992.1;
Genbank Nucleotide ID NM_001178777.1;
Protein Name Postreplication repair E3 ubiquitin-protein ligase RAD18
Protein Synonyms/Alias Radiation sensitivity protein 18;
Gene Name RAD18
Gene Synonyms/Alias YCR066W;YCR66W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 E3 RING-finger protein, member of the UBC2/RAD6 epistasis group. Associates to the E2 ubiquitin conjugating enzyme UBC2/RAD6 to form the UBC2-RAD18 ubiquitin ligase complex involved in postreplicative repair (PRR) of damaged DNA. The UBC2-RAD18 complex cooperates with RAD5 and the UBC13-MMS2 dimer to attach mono-ubiquitin chains on 'Lys-164' of POL30, which is necessary for PRR. The UBC2-RAD18 complex is also involved in prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine.
Phosphorylation Sites
 dbPAF PTMs: 15
PositionPeptidesSourceReferences ( PMIDs )
153ERKLAKRSMTDILPLdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22369663; 22817900;
155KLAKRSMTDILPLSSdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated17287358; 18407956; 16381945; 23193290; 19366988; 22817900
161MTDILPLSSKPSKRNcurated22817900; 19547744
165LPLSSKPSKRNFAMFcurated22817900; 19547744
174RNFAMFRSERIKKKSdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated17287358; 16381945; 23193290; 19366988; 22817900
181SERIKKKSKPNEQMAdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17287358; 16381945; 23193290; 19366988; 22817900
244SSRFKVRTPEVDKSSdbPTM 3.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22817900; 19547744
284NFTSMTQSQIKQKLSdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
416GSSDSSGSVGHSNDGcurated25315811
420SSGSVGHSNDGDGVEcurated25315811
431DGVEKVQSDQGTEDQPhosphoGRID 2.0;curated21126336; 20702584; 19779198; 26040289; 22369663; 22817900;
435KVQSDQGTEDQQMEKcurated25521595
464RNLPNEDSTDADLSRdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21126336; 19366988; 26040289;
465NLPNEDSTDADLSREPhosphoGRID 2.0;PhosphoPep 2.019779198; 19060867
470DSTDADLSRELMDLNPhosphoGRID 2.0;curated19779198; 25315811
Sequence
(Fasta)		MDHQITTASD FTTTSIPSLY QLDTLLRCHI CKDFLKVPVL TPCGHTFCSL CIRTHLNNQP 60
NCPLCLFEFR ESLLRSEFLV SEIIQSYTSL RSSLLDALRI PKPTPVPENE EVPGPENSSW 120
IELISESESD SVNAADDDLQ IVATSERKLA KRSMTDILPL SSKPSKRNFA MFRSERIKKK 180
SKPNEQMAQC PICQQFYPLK ALEKTHLDEC LTLQSLGKKP KISTTFPTES NPHNKSSSRF 240
KVRTPEVDKS SCGETSHVDK YLNSMMSAEH QRLPKINFTS MTQSQIKQKL SSLGLSTNGT 300
RQNMIKRYNH YEMLWNSNFC DSLEPVDEAE LKRQLLSWDV SHNKTPQNSS NKGGISKLMI 360
MKSNGKSSSY RKLLENFKND KFNRKGWMVM FRKDFARLIR EAKMKIKTGS SDSSGSVGHS 420
NDGDGVEKVQ SDQGTEDQQM EKDQDTVINE DRVAGERNLP NEDSTDADLS RELMDLNEYS 480
KDPPGNN 488
Keyword

KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0238--DNA-binding
KW-0436--Ligase
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR004580--Rad18
IPR003034--SAP_dom
IPR018957--Znf_C3HC4_RING-type
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS
PROSITE

PS50800--SAP
PS00518--ZF_RING_1
PS50089--ZF_RING_2
Pfam

PF02037--SAP
PF00097--zf-C3HC4
Gene Ontology

GO:0000790--C:nuclear chromatin
GO:0005634--C:nucleus
GO:0097505--C:Rad6-Rad18 complex
GO:0003684--F:damaged DNA binding
GO:0016874--F:ligase activity
GO:0003697--F:single-stranded DNA binding
GO:0061630--F:ubiquitin protein ligase activity
GO:0008270--F:zinc ion binding
GO:0042275--P:error-free postreplication DNA repair
GO:0070987--P:error-free translesion synthesis
GO:0042276--P:error-prone translesion synthesis
GO:0006513--P:protein monoubiquitination
GO:0009411--P:response to UV