dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005399
Uniprot Accession P10823; GPA2_YEAST; D3DLR9;
Genbank Protein ID NP_010937.3;
Genbank Nucleotide ID NM_001178911.3;
Protein Name Guanine nucleotide-binding protein alpha-2 subunit
Protein Synonyms/Alias GP2-alpha;
Gene Name GPA2
Gene Synonyms/Alias SSP101;YER020W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Alpha subunit of the heterotrimeric guanine nucleotide-binding protein (G protein) involved in glucose-induced cAMP signaling. Binds to its cognate transmembrane receptor GPR1, which senses extracellular carbon sources, and activates cAMP-PKA signaling and governs diploid pseudohyphal differentiation and haploid invasive growth. The G protein beta-mimic proteins GPB1 and GPB2 inhibit GPA2-GPR1 coupling, probably to reduce signaling in the absence of glucose.
Phosphorylation Sites
 dbPAF PTMs: 12
PositionPeptidesSourceReferences ( PMIDs )
12ASSEKNGSTPDTQTAPhosphoGRID 2.0;curated21126336; 25315811
13SSEKNGSTPDTQTAScurated26040406
16KNGSTPDTQTASAGScurated26040406; 22369663; 25315811
20TPDTQTASAGSDNVGdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated11875433; 16381945; 23193290; 19366988; 26040289; 22369663;
23TQTASAGSDNVGKAKdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated11875433; 16381945; 23193290; 19366988; 26040406; 22369663;
46KTVRTVNTANQQEKQcurated25315811
90ATANTSGSQQINIDSdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
111SNVAAQPSLSDASSGPhosphoGRID 2.0;PhosphoPep 2.0;curated19779198; 19060867; 26040289; 22369663; 25315811; 22817900
113VAAQPSLSDASSGSNPhosphoGRID 2.019779198
116QPSLSDASSGSNDKEPhosphoGRID 2.0;PHOSIDA;curated19779198; 21081558; 26040406; 19795423
117PSLSDASSGSNDKELdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;curated17563356; 16381945; 23193290; 19779198; 19060867; 22369663;
119LSDASSGSNDKELKVdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 20702584; 19779198; 21081558;
Sequence
(Fasta)		MGLCASSEKN GSTPDTQTAS AGSDNVGKAK VPPKQEPQKT VRTVNTANQQ EKQQQRQQQP 60
SPHNVKDRKE QNGSINNAIS PTATANTSGS QQINIDSALR DRSSNVAAQP SLSDASSGSN 120
DKELKVLLLG AGESGKSTVL QQLKILHQNG FSEQEIKEYI PLIYQNLLEI GRNLIQARTR 180
FNVNLEPECE LTQQDLSRTM SYEMPNNYTG QFPEDIAGVI STLWALPSTQ DLVNGPNASK 240
FYLMDSTPYF MENFTRITSP NYRPTQQDIL RSRQMTSGIF DTVIDMGSDI KMHIYDVGGQ 300
RSERKKWIHC FDNVTLVIFC VSLSEYDQTL MEDKNQNRFQ ESLVLFDNIV NSRWFARTSV 360
VLFLNKIDLF AEKLSKVPME NYFPDYTGGS DINKAAKYIL WRFVQLNRAN LSIYPHVTQA 420
TDTSNIRLVF AAIKETILEN TLKDSGVLQ
Keyword

KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0342--GTP-binding
KW-0449--Lipoprotein
KW-0460--Magnesium
KW-0472--Membrane
KW-0479--Metal-binding
KW-0519--Myristate
KW-0547--Nucleotide-binding
KW-0564--Palmitate
KW-1185--Reference proteome
KW-0807--Transducer
Interpro

IPR002975--Fungi_Gprotein_alpha
IPR001019--Gprotein_alpha_su
IPR011025--GproteinA_insert
IPR027417--P-loop_NTPase
PROSITE
Pfam

PF00503--G-alpha
Gene Ontology

GO:0005834--C:heterotrimeric G-protein complex
GO:0005886--C:plasma membrane
GO:0031683--F:G-protein beta/gamma-subunit complex binding
GO:0001664--F:G-protein coupled receptor binding
GO:0005525--F:GTP binding
GO:0003924--F:GTPase activity
GO:0046872--F:metal ion binding
GO:0004871--F:signal transducer activity
GO:0007189--P:adenylate cyclase-activating G-protein coupled receptor signaling pathway
GO:0030437--P:ascospore formation
GO:0010255--P:glucose mediated signaling pathway
GO:0009757--P:hexose mediated signaling
GO:0007124--P:pseudohyphal growth
GO:0001302--P:replicative cell aging
GO:0007165--P:signal transduction