dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005331
Uniprot Accession P10363; PRI1_YEAST; D6VVT8;
Genbank Protein ID NP_012273.1;
Genbank Nucleotide ID NM_001179530.1;
Protein Name DNA primase small subunit
Protein Synonyms/Alias DNA polymerase alpha:primase complex p48 subunit;DNA polymerase-primase complex p48 subunit;Pol alpha-primase complex p48 subunit;DNA primase 48 kDa subunit;
Gene Name PRI1
Gene Synonyms/Alias YIR008C;YIB8C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. In a complex with DNA polymerase alpha (DNA polymerase alpha:primase) constitutes a replicative polymerase. Both primase components participate in formation of the active center, but the ATP-binding site is exclusively located on p48.
Phosphorylation Sites
 dbPAF PTMs: 2
PositionPeptidesSourceReferences ( PMIDs )
61GAYKRYNSFNSVQDFdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
406EDDDEPASLDF----dbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21126336; 19366988; 22369663;
Sequence
(Fasta)		MTNSVKTNGP SSSDMEYYYK SLYPFKHIFN WLNHSPKPSR DMINREFAMA FRSGAYKRYN 60
SFNSVQDFKA QIEKANPDRF EIGAIYNKPP RERDTLLKSE LKALEKELVF DIDMDDYDAF 120
RTCCSGAQVC SKCWKFISLA MKITNTALRE DFGYKDFIWV FSGRRGAHCW VSDKRARALT 180
DVQRRNVLDY VNVIRDRNTD KRLALKRPYH PHLARSLEQL KPFFVSIMLE EQNPWEDDQH 240
AIQTLLPALY DKQLIDSLKK YWLDNPRRSS KEKWNDIDQI ATSLFKGPKQ DSHIIKLREC 300
KEDLVLMTLY PKLDVEVTKQ TIHLLKAPFC IHPATGNVCV PIDESFAPEK APKLIDLQTE 360
MEKNNDVSLT ALQPFINQFQ AYVSSLLKNE LGSVKRERED DDEPASLDF
Keyword

KW-0002--3D-structure
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0235--DNA replication
KW-0240--DNA-directed RNA polymerase
KW-0479--Metal-binding
KW-0547--Nucleotide-binding
KW-0548--Nucleotidyltransferase
KW-0639--Primosome
KW-1185--Reference proteome
KW-0804--Transcription
KW-0808--Transferase
KW-0862--Zinc
Interpro

IPR002755--DNA_primase_S
IPR014052--DNA_primase_ssu_euk/arc
PROSITE
Pfam

PF01896--DNA_primase_S
Gene Ontology

GO:0005658--C:alpha DNA polymerase:primase complex
GO:0043596--C:nuclear replication fork
GO:1990077--C:primosome complex
GO:0005524--F:ATP binding
GO:0003896--F:DNA primase activity
GO:0046872--F:metal ion binding
GO:0006260--P:DNA replication
GO:0006270--P:DNA replication initiation
GO:0006269--P:DNA replication, synthesis of RNA primer
GO:0006273--P:lagging strand elongation