dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0005329
Uniprot Accession P10361; P53_RAT; O09168; Q4KMA9; Q66HM0;
Genbank Protein ID NP_112251.2; XP_006246656.1; XP_006246657.1;
Genbank Nucleotide ID NM_030989.3; XM_006246594.2; XM_006246595.2;
Protein Name Cellular tumor antigen p53
Protein Synonyms/Alias Tumor suppressor p53;
Gene Name Tp53
Gene Synonyms/Alias P53;
Organism Rattus norvegicus(Rat)
NCBI Taxa ID 10116
Functional Description
(View all)
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
Phosphorylation Sites
dbPAF PTMs: 12
PositionPeptidesSourceReferences ( PMIDs )
4----MEDSQSDMSIEdbPTM 3.0;PhosphoSitePlus18697815; 16381945; 23193290; 22135298
6--MEDSQSDMSIELPdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus7862459; 16381945; 23193290; 18697815; 22135298
9EDSQSDMSIELPLSQdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus7862459; 16381945; 23193290; 22135298
15MSIELPLSQETFSCLdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;curated7862459; 16381945; 23193290; 11574421; 12534344; 12644594; 1
18ELPLSQETFSCLWKLPhosphoSitePlus22135298
20PLSQETFSCLWKLLPdbPTM 3.0;PhosphoSitePlus;curated12919932; 16330492; 16381945; 23193290; 22135298; 22817900
39LPTTATGSPNSMEDLdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;curated7862459; 16381945; 23193290; 16330492; 22135298; 22817900
308SAKRALPTSTSSSPQcurated23984901
309AKRALPTSTSSSPQQcurated23984901
313LPTSTSSSPQQKKKPdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;curated7862459; 16381945; 23193290; 18697815; 22135298; 22817900
376TKKGQSTSRHKKPMIdbPTM 3.0;PhosphoSitePlus;curated11574421; 16381945; 23193290; 22135298; 22817900
390IKKVGPDSD------dbPTM 3.0;PhosphoSitePlus11574421; 18697815; 16381945; 23193290; 22135298
Sequence
(Fasta)
MEDSQSDMSI ELPLSQETFS CLWKLLPPDD ILPTTATGSP NSMEDLFLPQ DVAELLEGPE 60
EALQVSAPAA QEPGTEAPAP VAPASATPWP LSSSVPSQKT YQGNYGFHLG FLQSGTAKSV 120
MCTYSISLNK LFCQLAKTCP VQLWVTSTPP PGTRVRAMAI YKKSQHMTEV VRRCPHHERC 180
SDGDGLAPPQ HLIRVEGNPY AEYLDDRQTF RHSVVVPYEP PEVGSDYTTI HYKYMCNSSC 240
MGGMNRRPIL TIITLEDSSG NLLGRDSFEV RVCACPGRDR RTEEENFRKK EEHCPELPPG 300
SAKRALPTST SSSPQQKKKP LDGEYFTLKI RGRERFEMFR ELNEALELKD ARAAEESGDS 360
RAHSSYPKTK KGQSTSRHKK PMIKKVGPDS D 392
Keyword

KW-0007--Acetylation
KW-0010--Activator
KW-0053--Apoptosis
KW-0090--Biological rhythms
KW-0131--Cell cycle
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0238--DNA-binding
KW-0256--Endoplasmic reticulum
KW-1017--Isopeptide bond
KW-0479--Metal-binding
KW-0488--Methylation
KW-0496--Mitochondrion
KW-1210--Necrosis
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0043--Tumor suppressor
KW-0832--Ubl conjugation
KW-0862--Zinc

Interpro

IPR008967--p53-like_TF_DNA-bd
IPR012346--p53/RUNT-type_TF_DNA-bd
IPR011615--p53_DNA-bd
IPR010991--p53_tetrameristn
IPR013872--p53_transactivation_domain
IPR002117--p53_tumour_suppressor

PROSITE

PS00348--P53

Pfam

PF00870--P53
PF08563--P53_TAD
PF07710--P53_tetramer

Gene Ontology

GO:0000785--C:chromatin
GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005783--C:endoplasmic reticulum
GO:0005759--C:mitochondrial matrix
GO:0005739--C:mitochondrion
GO:0005730--C:nucleolus
GO:0005634--C:nucleus
GO:0005657--C:replication fork
GO:0005667--C:transcription factor complex
GO:0005524--F:ATP binding
GO:0003682--F:chromatin binding
GO:0005507--F:copper ion binding
GO:0001046--F:core promoter sequence-specific DNA binding
GO:0003684--F:damaged DNA binding
GO:0003677--F:DNA binding
GO:0003690--F:double-stranded DNA binding
GO:0097371--F:MDM2/MDM4 family protein binding
GO:0002039--F:p53 binding
GO:0008022--F:protein C-terminus binding
GO:0000981--F:RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0043565--F:sequence-specific DNA binding
GO:0001221--F:transcription cofactor binding
GO:0003700--F:transcription factor activity, sequence-specific DNA binding
GO:0031625--F:ubiquitin protein ligase binding
GO:0007568--P:aging
GO:0007569--P:cell aging
GO:0034605--P:cellular response to heat
GO:0071417--P:cellular response to organonitrogen compound
GO:0034644--P:cellular response to UV
GO:0048512--P:circadian behavior
GO:0006978--P:DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
GO:0000733--P:DNA strand renaturation
GO:0043153--P:entrainment of circadian clock by photoperiod
GO:0042771--P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
GO:0031571--P:mitotic G1 DNA damage checkpoint
GO:0007275--P:multicellular organismal development
GO:0030308--P:negative regulation of cell growth
GO:2000279--P:negative regulation of DNA biosynthetic process
GO:0048662--P:negative regulation of smooth muscle cell proliferation
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0006289--P:nucleotide-excision repair
GO:0097252--P:oligodendrocyte apoptotic process
GO:0043065--P:positive regulation of apoptotic process
GO:0045787--P:positive regulation of cell cycle
GO:0031065--P:positive regulation of histone deacetylation
GO:0002687--P:positive regulation of leukocyte migration
GO:0043525--P:positive regulation of neuron apoptotic process
GO:0090200--P:positive regulation of release of cytochrome c from mitochondria
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0051262--P:protein tetramerization
GO:1903205--P:regulation of hydrogen peroxide-induced cell death
GO:0051453--P:regulation of intracellular pH
GO:0006357--P:regulation of transcription from RNA polymerase II promoter
GO:0043200--P:response to amino acid
GO:0031000--P:response to caffeine
GO:0034097--P:response to cytokine
GO:0042493--P:response to drug
GO:0010332--P:response to gamma radiation
GO:0055093--P:response to hyperoxia
GO:0010035--P:response to inorganic substance
GO:0010038--P:response to metal ion
GO:0014070--P:response to organic cyclic compound
GO:0010243--P:response to organonitrogen compound
GO:0006979--P:response to oxidative stress
GO:0032526--P:response to retinoic acid
GO:0009411--P:response to UV
GO:0010224--P:response to UV-B
GO:0033552--P:response to vitamin B3
GO:0010165--P:response to X-ray
GO:0006351--P:transcription, DNA-templated
GO:0042060--P:wound healing