dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005296
Uniprot Accession P10081; IF4A_YEAST; D6VW46;
Genbank Protein ID NP_012397.1; NP_012985.3;
Genbank Nucleotide ID NM_001181571.1; NM_001179849.3;
Protein Name ATP-dependent RNA helicase eIF4A
Protein Synonyms/Alias Eukaryotic initiation factor 4A;eIF-4A;Stimulator factor I 37 kDa component;Translation initiation factor 1/2;p37;
Gene Name TIF2
Gene Synonyms/Alias TIF41A;YKR059W;TIF41B;YJL138C;J0660;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Phosphorylation Sites
 dbPAF PTMs: 14
PositionPeptidesSourceReferences ( PMIDs )
2------MSEGITDIEdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;SysPTM 2.0;curated15665377; 16381945; 23193290; 21126336; 21081558; 19366988;
6--MSEGITDIEESQIcurated26040289; 22369663; 19823750; 24961812
11GITDIEESQIQTNYDcurated26040289; 22817900; 23607784
15IEESQIQTNYDKVVYcurated26040289
17ESQIQTNYDKVVYKFcurated26040289
68DVLAQAQSGTGKTGTPhosphoGRID 2.0;PhosphoPep 2.019779198; 19060867
70LAQAQSGTGKTGTFSPhosphoPep 2.019060867
73AQSGTGKTGTFSIAAPhosphoGRID 2.0;UniProt;curated19779198; 22817900
77TGKTGTFSIAALQRIUniProt;curated19779198; 22817900
86AALQRIDTSVKAPQAPhosphoPep 2.0;curated19060867; 24961812
87ALQRIDTSVKAPQALPhosphoPep 2.019060867
128VHACIGGTSFVEDAEdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
129HACIGGTSFVEDAEGdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19366988; 22817900;
146DAQIVVGTPGRVFDNdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 21126336; 20702584;
Sequence
(Fasta)		MSEGITDIEE SQIQTNYDKV VYKFDDMELD ENLLRGVFGY GFEEPSAIQQ RAIMPIIEGH 60
DVLAQAQSGT GKTGTFSIAA LQRIDTSVKA PQALMLAPTR ELALQIQKVV MALAFHMDIK 120
VHACIGGTSF VEDAEGLRDA QIVVGTPGRV FDNIQRRRFR TDKIKMFILD EADEMLSSGF 180
KEQIYQIFTL LPPTTQVVLL SATMPNDVLE VTTKFMRNPV RILVKKDELT LEGIKQFYVN 240
VEEEEYKYEC LTDLYDSISV TQAVIFCNTR RKVEELTTKL RNDKFTVSAI YSDLPQQERD 300
TIMKEFRSGS SRILISTDLL ARGIDVQQVS LVINYDLPAN KENYIHRIGR GGRFGRKGVA 360
INFVTNEDVG AMRELEKFYS TQIEELPSDI ATLLN 396
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0347--Helicase
KW-0378--Hydrolase
KW-0396--Initiation factor
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
KW-0694--RNA-binding
Interpro

IPR011545--DEAD/DEAH_box_helicase_dom
IPR014001--Helicase_ATP-bd
IPR001650--Helicase_C
IPR027417--P-loop_NTPase
IPR000629--RNA-helicase_DEAD-box_CS
IPR014014--RNA_helicase_DEAD_Q_motif
PROSITE

PS00039--DEAD_ATP_HELICASE
PS51192--HELICASE_ATP_BIND_1
PS51194--HELICASE_CTER
PS51195--Q_MOTIF
Pfam

PF00270--DEAD
PF00271--Helicase_C
Gene Ontology

GO:0005737--C:cytoplasm
GO:0016281--C:eukaryotic translation initiation factor 4F complex
GO:0005840--C:ribosome
GO:0005524--F:ATP binding
GO:0004004--F:ATP-dependent RNA helicase activity
GO:0008186--F:RNA-dependent ATPase activity
GO:0003743--F:translation initiation factor activity
GO:0010468--P:regulation of gene expression
GO:0006446--P:regulation of translational initiation
GO:0010501--P:RNA secondary structure unwinding
GO:0006413--P:translational initiation