dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005138
Uniprot Accession P0CE41; HAP1_YEAST; D6VYQ2; P12351; Q06574; Q6BD21;
Genbank Protein ID NP_013357.1;
Genbank Nucleotide ID NM_001182143.1;
Protein Name Heme-responsive zinc finger transcription factor HAP1
Protein Synonyms/Alias CYP1 activatory protein;Heme activator protein 1;
Gene Name HAP1
Gene Synonyms/Alias CYP1;YLR256W;L9672.1;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Regulation of oxygen dependent gene expression. It modulates the expression of Iso-1 (CYP1) and Iso-2 (CYP3) cytochrome c. In response to heme, promotes transcription of genes encoding functions required for respiration, controlling oxidative damage and repression of anaerobic genes. Binds to the sequence 5'-CGGNNNTNNCGG-3' (By similarity). Is non-functional in terms of iso-1 cytochrome c expression in strain S288c and its derivatives.
Phosphorylation Sites
 dbPAF PTMs: 20
PositionPeptidesSourceReferences ( PMIDs )
6--MSNTPYNSSVPSIcurated26040289
8MSNTPYNSSVPSIAScurated26040289
9SNTPYNSSVPSIASMcurated26040289
12PYNSSVPSIASMTQScurated26040289
41GANTSSNSPPLHMSSPhosphoPep 2.0;curated19060867; 22817900
48SPPLHMSSDSSKIKRcurated22817900
134TLSKVHSSPSSNSLKPhosphoPep 2.019060867
136SKVHSSPSSNSLKSYdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 19366988; 22369663;
137KVHSSPSSNSLKSYNdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
139HSSPSSNSLKSYNTPdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 22369663; 25315811
205NANSSSLSISNKYDNcurated25315811
318MVAPKEETPLPRKCPcurated25315811
743NNMSAATTNSSSRSGcurated25315811
745MSAATTNSSSRSGSAcurated25315811
756SGSADSRSGSNPVNKcurated25315811
758SADSRSGSNPVNKKEcurated25315811
936YADGRNTSSSDIDKKcurated25315811
1180HPKVSNMSGFLAGDKdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 25315811
1242IRSYKPITYTSSNLRcurated22817900
1282NDIPRGISPKPSNGLdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
Sequence
(Fasta)		MSNTPYNSSV PSIASMTQSS VSRSPNMHTA TTPGANTSSN SPPLHMSSDS SKIKRKRNRI 60
PLSCTICRKR KVKCDKLRPH CQQCTKTGVA HLCHYMEQTW AEEAEKELLK DNELKKLRER 120
VKSLEKTLSK VHSSPSSNSL KSYNTPESSN LFMGSDEHTT LVNANTGSAS SASHMHQQQQ 180
QQQQQEQQQD FSRSANANAN SSSLSISNKY DNDELDLTKD FDLLHIKSNG TIHLGATHWL 240
SIMKGDPYLK LLWGHIFAMR EKLNEWYYQK NSYSKLKSSK CPINHAQAPP SAAAAATRKC 300
PVDHSAFSSG MVAPKEETPL PRKCPVDHTM FSSGMIPPRE DTSSQKRCPV DHTMYSAGMM 360
PPKDETPSPF STKAMIDHNK HTMNPPQSKC PVDHRNYMKD YPSDMANSSS NPASRCPIDH 420
SSMKNTAALP ASTHNTIPHH QPQSGSHARS HPAQSRKHDS YMTESEVLAT LCEMLPPKRV 480
IALFIEKFFK HLYPAIPILD EQNFKNHVNQ MLSLSSMNPT VNNFGMSMPS SSTLENQPIT 540
QINLPKLSDS CNLGILIIIL RLTWLSIPSN SCEVDLGEES GSFLVPNESS NMSASALTSM 600
AKEESLLLKH ETPVEALELC QKYLIKFDEL SSISNNNVNL TTVQFAIFYN FYMKSASNDL 660
TTLTNTNNTG MANPGHDSES HQILLSNITQ MAFSCGLHRD PDNFPQLNAT IPATSQDVSN 720
NGSKKANPST NPTLNNNMSA ATTNSSSRSG SADSRSGSNP VNKKENQVSI ERFKHTWRKI 780
WYYIVSMDVN QSLSLGSPRL LRNLRDFSDT KLPSASRIDY VRDIKELIIV KNFTLFFQID 840
LCIIAVLNHI LNVSLARSVR KFELDSLINL LKNLTYGTEN VNDVVSSLIN KGLLPTSEGG 900
SVDSNNDEIY GLPKLPDILN HGQHNQNLYA DGRNTSSSDI DKKLDLPHES TTRALFFSKH 960
MTIRMLLYLL NYILFTHYEP MGSEDPGTNI LAKEYAQEAL NFAMDGYRNC MIFFNNIRNT 1020
NSLFDYMNVI LSYPCLDIGH RSLQFIVCLI LRAKCGPLTG MRESSIITNG TSSGFNSSVE 1080
DEDVKVKQES SDELKKDDFM KDVNLDSGDS LAEILMSRML LFQKLTKQLS KKYNYAIRMN 1140
KSTGFFVSLL DTPSKKSDSK SGGSSFMLGN WKHPKVSNMS GFLAGDKDQL QKCPVYQDAL 1200
GFVSPTGANE GSAPMQGMSL QGSTARMGGT QLPPIRSYKP ITYTSSNLRR MNETGEAEAK 1260
RRRFNDGYID NNSNNDIPRG ISPKPSNGLS SVQPLLSSFS MNQLNGGTIP TVPSLTNITS 1320
QMGALPSLDR ITTNQINLPD PSRDEAFDNS IKQMTPMTSA FMNANTTIPS STLNGNMNMN 1380
GAGTANTDTS ANGSALSTLT SPQGSDLASN SATQYKPDLE DFLMQNSNFN GLMINPSSLV 1440
EVVGGYNDPN NLGRNDAVDF LPVDNVEIDG VGIKINYHLL TSIYVTSILS YTVLEDDAND 1500
EK 1503
Keyword

KW-0010--Activator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-0349--Heme
KW-0408--Iron
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0677--Repeat
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0862--Zinc
Interpro

IPR007219--Transcription_factor_dom_fun
IPR001138--Zn2-C6_fun-type_DNA-bd
PROSITE

PS00463--ZN2_CY6_FUNGAL_1
PS50048--ZN2_CY6_FUNGAL_2
Pfam

PF00172--Zn_clus
Gene Ontology

GO:0005739--C:mitochondrion
GO:0005634--C:nucleus
GO:0000978--F:RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0001077--F:transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0008270--F:zinc ion binding
GO:0000436--P:carbon catabolite activation of transcription from RNA polymerase II promoter
GO:0071169--P:establishment of protein localization to chromatin
GO:0061428--P:negative regulation of transcription from RNA polymerase II promoter in response to hypoxia
GO:0043457--P:regulation of cellular respiration
GO:0006366--P:transcription from RNA polymerase II promoter