dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0005048
Uniprot Accession P0C2J3; YL21B_YEAST; D6VZ45;
Genbank Protein ID NP_058174.3;
Genbank Nucleotide ID NM_001184411.4;
Protein Name Transposon Ty2-LR1 Gag-Pol polyprotein
Protein Synonyms/Alias TY2A-TY2B;Transposon Ty2 TYA-TYB polyprotein;
Gene Name TY2B-LR1
Gene Synonyms/Alias YLRWTy2-1 POL;YLR410W-B;L9931.7b;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the retrotransposons dimeric RNA genome. The particles are assembled from trimer-clustered units and there are holes in the capsid shells that allow for the diffusion of macromolecules. CA has also nucleocapsid-like chaperone activity, promoting primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty2 RNA and initiation of reverse transcription (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 18
PositionPeptidesSourceReferences ( PMIDs )
422ESTVSSQYLSDDNELPhosphoGRID 2.017330950
424TVSSQYLSDDNELSLcurated22369663; 22817900; 24961812; 18407956
570SKKYLIPSHISKLTIcurated22817900
987SEYNILPSPVRSRTPPhosphoPep 2.0;curated19060867; 22817900
993PSPVRSRTPHIINKEPhosphoGRID 2.017330950
1014TIESDTTSPRHSSTFPhosphoGRID 2.0;PhosphoPep 2.0;curated17330950; 19060867; 22369663; 24603354; 22817900; 24961812;
1031RNQKRPGSPNDMIDLPhosphoGRID 2.0;PhosphoPep 2.0;curated17330950; 19060867; 26040289; 22817900; 20377248; 18407956
1068EPYIQRNSDTNIKYRcurated24961812
1079IKYRTTNSTPSIDDRcurated18407956
1082RTTNSTPSIDDRSSNPhosphoGRID 2.0;curated19779198; 26040289
1087TPSIDDRSSNSESTTPhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 17330950; 19060867; 19366988
1088PSIDDRSSNSESTTPPhosphoPep 2.0;curated19060867; 26040289
1090IDDRSSNSESTTPIIPhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 19060867; 19366988; 24961812
1093RSSNSESTTPIISIESysPTM 2.017330950; 19366988
1094SSNSESTTPIISIETPhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 19060867; 19366988; 26040289; 24961812
1110AVCDNTPSIDTDPPEPhosphoGRID 2.0;curated19779198; 22817900
1125YRSSDHATPNIMPDKPhosphoGRID 2.0;PhosphoPep 2.017330950; 19060867
1155LPDLTHKSPTDTSDVcurated25315811
Sequence
(Fasta)		MESQQLHQNP HSLHGSAYAS VTSKEVPSNQ DPLAVSASNL PEFDRDSTKV NSQQETTPGT 60
SAVPENHHHV SPQPASVPPP QNGQYQQHGM MTPNKAMASN WAHYQQPSMM TCSHYQTSPA 120
YYQPDPHYPL PQYIPPLSTS SPDPIDLKNQ HSEIPQAKTK VGNNVLPPHT LTSEENFSTW 180
VKFYIRFLKN SNLGDIIPND QGEIKRQMTY EEHAYIYNTF QAFAPFHLLP TWVKQILEIN 240
YADILTVLCK SVSKMQTNNQ ELKDWIALAN LEYDGSTSAD TFEITVSTII QRLKENNINV 300
SDRLACQLIL KGLSGDFKYL RNQYRTKTNM KLSQLFAEIQ LIYDENKIMN LNKPSQYKQH 360
SEYKNVSRTS PNTTNTKVTT RNYHRTNSSK PRAAKAHNIA TSSKFSRVNN DHINESTVSS 420
QYLSDDNELS LGQQQKESKP THTIDSNDEL PDHLLIDSGA SQTLVRSAHY LHHATPNSEI 480
NIVDAQKQDI PINAIGNLHF NFQNGTKTSI KALHTPNIAY DLLSLSELAN QNITACFTRN 540
TLERSDGTVL APIVKHGDFY WLSKKYLIPS HISKLTINNV NKSKSVNKYP YPLIHRMLGH 600
ANFRSIQKSL KKNAVTYLKE SDIEWSNAST YQCPDCLIGK STKHRHVKGS RLKYQESYEP 660
FQYLHTDIFG PVHHLPKSAP SYFISFTDEK TRFQWVYPLH DRREESILNV FTSILAFIKN 720
QFNARVLVIQ MDRGSEYTNK TLHKFFTNRG ITACYTTTAD SRAHGVAERL NRTLLNDCRT 780
LLHCSGLPNH LWFSAVEFST IIRNSLVSPK NDKSARQHAG LAGLDITTIL PFGQPVIVNN 840
HNPDSKIHPR GIPGYALHPS RNSYGYIIYL PSLKKTVDTT NYVILQDNQS KLDQFNYDTL 900
TFDDDLNRLT AHNQSFIEQN ETEQSYDQNT ESDHDYQSEI EINSDPLVND FSSQSMNPLQ 960
LDHEPVQKVR APKEVDADIS EYNILPSPVR SRTPHIINKE STEMGGTIES DTTSPRHSST 1020
FTARNQKRPG SPNDMIDLTS QDRVNYGLEN IKTTRLGGTE EPYIQRNSDT NIKYRTTNST 1080
PSIDDRSSNS ESTTPIISIE TKAVCDNTPS IDTDPPEYRS SDHATPNIMP DKSSKNVTAD 1140
SILDDLPLPD LTHKSPTDTS DVSKDIPHIH SRQTNSSLGG MDDSNVLTTT KSKKRSLEDN 1200
ETEIEVSRDT WNNKNMRSLE PPRSKKRINL IAAIKGVKSI KPVRTTLRYD EAITYNKDNK 1260
EKDRYVEAYH KEISQLLKMN TWDTNKYYDR NDIDPKKVIN SMFIFNKKRD GTHKARFVAR 1320
GDIQHPDTYD SDMQSNTVHH YALMTSLSIA LDNDYYITQL DISSAYLYAD IKEELYIRPP 1380
PHLGLNDKLL RLRKSLYGLK QSGANWYETI KSYLINCCDM QEVRGWSCVF KNSQVTICLF 1440
VDDMILFSKD LNANKKIITT LKKQYDTKII NLGERDNEIQ YDILGLEIKY QRSKYMKLGM 1500
EKSLTEKLPK LNVPLNPKGK KLRAPGQPGH YIDQDELEID EDEYKEKVHE MQKLIGLASY 1560
VGYKFRFDLL YYINTLAQHI LFPSRQVLDM TYELIQFMWD TRDKQLIWHK NKPTKPDNKL 1620
VAISDASYGN QPYYKSQIGN IFLLNGKVIG GKSTKASLTC TSTTEAEIHA VSEAIPLLNN 1680
LSHLVQELNK KPIIKGLLTD SRSTISIIKS TNEEKFRNRF FGTKAMRLRD EVSGNNLYVY 1740
YIETKKNIAD VMTKPLPIKT FKLLTNKWIH 1771
Keyword

KW-0064--Aspartyl protease
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0229--DNA integration
KW-0233--DNA recombination
KW-0238--DNA-binding
KW-0239--DNA-directed DNA polymerase
KW-0255--Endonuclease
KW-0378--Hydrolase
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0511--Multifunctional enzyme
KW-0540--Nuclease
KW-0547--Nucleotide-binding
KW-0548--Nucleotidyltransferase
KW-0539--Nucleus
KW-0645--Protease
KW-1185--Reference proteome
KW-0688--Ribosomal frameshifting
KW-0694--RNA-binding
KW-0695--RNA-directed DNA polymerase
KW-0808--Transferase
KW-0814--Transposable element
KW-0815--Transposition
KW-0917--Virion maturation
KW-1188--Virus exit from host cell
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR025724--GAG-pre-integrase_dom
IPR001584--Integrase_cat-core
IPR015820--Retrotransposon_Ty1A_N
IPR012337--RNaseH-like_dom
IPR013103--RVT_2
PROSITE

PS50994--INTEGRASE
Pfam

PF13976--gag_pre-integrs
PF00665--rve
PF07727--RVT_2
PF01021--TYA
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0000943--C:retrotransposon nucleocapsid
GO:0004190--F:aspartic-type endopeptidase activity
GO:0005524--F:ATP binding
GO:0003677--F:DNA binding
GO:0003887--F:DNA-directed DNA polymerase activity
GO:0046872--F:metal ion binding
GO:0008233--F:peptidase activity
GO:0004540--F:ribonuclease activity
GO:0003723--F:RNA binding
GO:0003964--F:RNA-directed DNA polymerase activity
GO:0004523--F:RNA-DNA hybrid ribonuclease activity
GO:0071897--P:DNA biosynthetic process
GO:0015074--P:DNA integration
GO:0006310--P:DNA recombination
GO:0090501--P:RNA phosphodiester bond hydrolysis
GO:0006278--P:RNA-dependent DNA replication
GO:0032197--P:transposition, RNA-mediated
GO:0019076--P:viral release from host cell