dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004993
Uniprot Accession P09932; HO_YEAST; D6VRC8; Q12183;
Genbank Protein ID NP_010054.1;
Genbank Nucleotide ID NM_001180287.1;
Protein Name Homothallic switching endonuclease
Protein Synonyms/Alias
Gene Name HO
Gene Synonyms/Alias YDL227C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Initiation of mating type interconversion. This protein is a site-specific endonuclease that cleaves a site in the mat locus on chromosome III. The double-strand break is followed by a unidirectional gene conversion event that replaces the information at the mat locus by information copied from either of the two homologous loci (HMR and HML) that reside at the extremity of the chromosome III. Endonuclease expression takes place in late G1 just before cells enter S phase.
Phosphorylation Sites
 dbPAF PTMs: 3
PositionPeptidesSourceReferences ( PMIDs )
225LWLGDSTTKEPEISVPhosphoGRID 2.018807043; 14506225
352SYKIAIQTVYSSIMDcurated22817900
355IAIQTVYSSIMDGIVcurated22817900
Sequence
(Fasta)		MLSENTTILM ANGEIKDIAN VTANSYVMCA DGSAARVINV TQGYQKIYNI QQKTKHRAFE 60
GEPGRLDPRR RTVYQRLALQ CTAGHKLSVR VPTKPLLEKS GRNATKYKVR WRNLQQCQTL 120
DGRIIIIPKN HHKTFPMTVE GEFAAKRFIE EMERSKGEYF NFDIEVRDLD YLDAQLRISS 180
CIRFGPVLAG NGVLSKFLTG RSDLVTPAVK SMAWMLGLWL GDSTTKEPEI SVDSLDPKLM 240
ESLRENAKIW GLYLTVCDDH VPLRAKHVRL HYGDGPDENR KTRNLRKNNP FWKAVTILKF 300
KRDLDGEKQI PEFMYGEHIE VREAFLAGLI DSDGYVVKKG EGPESYKIAI QTVYSSIMDG 360
IVHISRSLGM SATVTTRSAR EEIIEGRKVQ CQFTYDCNVA GGTTSQNVLS YCRSGHKTRE 420
VPPIIKREPV YFSFTDDFQG ESTVYGLTIE GHKNFLLGNK IEVKSCRGCC VGEQLKISQK 480
KNLKHCVACP RKGIKYFYKD WSGKNRVCAR CYGRYKFSGH HCINCKYVPE AREVKKAKDK 540
GEKLGITPEG LPVKGPECIK CGGILQFDAV RGPHKSCGNN AGARIC 587
Keyword

KW-0181--Complete proteome
KW-0238--DNA-binding
KW-0255--Endonuclease
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0540--Nuclease
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR028992--Hedgehog/Intein_dom
IPR007868--Hom_end_hint
IPR007869--Homing_endonuc_PI-Sce
IPR027434--Homing_endonucl
IPR006142--INTEIN
IPR004042--Intein_endonuc
PROSITE

PS50819--INTEIN_ENDONUCLEASE
Pfam

PF05204--Hom_end
PF05203--Hom_end_hint
Gene Ontology

GO:0005634--C:nucleus
GO:0003677--F:DNA binding
GO:0004519--F:endonuclease activity
GO:0046872--F:metal ion binding
GO:0000728--P:gene conversion at mating-type locus, DNA double-strand break formation
GO:0016539--P:intein-mediated protein splicing
GO:0007533--P:mating type switching