| Tag |
Content |
| dbPAF ID |
dbPAF-0004982 |
| Uniprot Accession |
P09880; TRNL_YEAST; D6VW97;
|
| Genbank Protein ID |
NP_012448.1;
|
| Genbank Nucleotide ID |
NM_001181520.1;
|
| Protein Name |
tRNA ligase |
| Protein Synonyms/Alias |
|
| Gene Name |
TRL1 |
| Gene Synonyms/Alias |
RLG1;YJL087C;J0927; |
| Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast) |
| NCBI Taxa ID |
559292 |
Functional Description
(View all) |
One of the two proteins required for the splicing of precursor tRNA molecules containing introns. The ligation activity requires three enzymatic activities: phosphorylation of the 5' terminus of the 3' half-tRNA in the presence of ATP, opening of the 2'3'-cyclic phosphodiester bond of the 5' half-tRNA leaving a 2'-phosphomonoester and ligation of the two tRNA halves in an ATP-dependent reaction.Functional Description
One of the two proteins required for the splicing of precursor tRNA molecules containing introns. The ligation activity requires three enzymatic activities: phosphorylation of the 5' terminus of the 3' half-tRNA in the presence of ATP, opening of the 2'3'-cyclic phosphodiester bond of the 5' half-tRNA leaving a 2'-phosphomonoester and ligation of the two tRNA halves in an ATP-dependent reaction. |
Phosphorylation Sites
|
dbPAF PTMs: 2 |
Sequence
(Fasta) | MPSPYDGKRT VTQLVNELEK AEKLSGRGRA YRRVCDLSHS NKKVISWKFN EWDYGKNTIT 60
LPCNARGLFI SDDTTNPVIV ARGYDKFFNV GEVNFTKWNW IEENCTGPYD VTIKANGCII 120
FISGLEDGTL VVCSKHSTGP RADVDRNHAE AGEKQLLRQL AAMNINRSDF ARMLYTHNVT 180
AVAEYCDDSF EEHILEYPLE KAGLYLHGVN VNKAEFETWD MKDVSQMASK YGFRCVQCIT 240
SNTLEDLKKF LDNCSATGSF EGQEIEGFVI RCHLKSTEKP FFFKYKFEEP YLMYRQWREV 300
TKDYISNKSR VFKFRKHKFI TNKYLDFAIP ILESSPKICE NYLKGFGVIE LRNKFLQSYG 360
MSGLEILNHE KVAELELKNA IDYDKVDERT KFLIFPISVI GCGKTTTSQT LVNLFPDSWG 420
HIQNDDITGK DKSQLMKKSL ELLSKKEIKC VIVDRNNHQF RERKQLFEWL NELKEDYLVY 480
DTNIKVIGVS FAPYDKLSEI RDITLQRVIK RGNNHQSIKW DELGEKKVVG IMNGFLKRYQ 540
PVNLDKSPDN MFDLMIELDF GQADSSLTNA KQILNEIHKA YPILVPEIPK DDEIETAFRR 600
SLDYKPTVRK IVGKGNNNQQ KTPKLIKPTY ISAKIENYDE IIELVKRCIA SDAELTEKFK 660
HLLASGKVQK ELHITLGHVM SSREKEAKKL WKSYCNRYTD QITEYNNNRI ENAQGSGNNQ 720
NTQVKTTDKL NFRLEKLCWD EKIIAIVVEL SKDKDGCIID ENNEKIKGLC CQNKIPHITL 780
CKLESGVKAV YSNVLCEKVE SAEVDENIKV VKLDNSKEFV GSVYLNF
828Fasta Sequence
>P09880|TRL1|Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
MPSPYDGKRTVTQLVNELEKAEKLSGRGRAYRRVCDLSHSNKKVISWKFNEWDYGKNTITLPCNARGLFISDDTTNPVIVARGYDKFFNVGEVNFTKWNWIEENCTGPYDVTIKANGCIIFISGLEDGTLVVCSKHSTGPRADVDRNHAEAGEKQLLRQLAAMNINRSDFARMLYTHNVTAVAEYCDDSFEEHILEYPLEKAGLYLHGVNVNKAEFETWDMKDVSQMASKYGFRCVQCITSNTLEDLKKFLDNCSATGSFEGQEIEGFVIRCHLKSTEKPFFFKYKFEEPYLMYRQWREVTKDYISNKSRVFKFRKHKFITNKYLDFAIPILESSPKICENYLKGFGVIELRNKFLQSYGMSGLEILNHEKVAELELKNAIDYDKVDERTKFLIFPISVIGCGKTTTSQTLVNLFPDSWGHIQNDDITGKDKSQLMKKSLELLSKKEIKCVIVDRNNHQFRERKQLFEWLNELKEDYLVYDTNIKVIGVSFAPYDKLSEIRDITLQRVIKRGNNHQSIKWDELGEKKVVGIMNGFLKRYQPVNLDKSPDNMFDLMIELDFGQADSSLTNAKQILNEIHKAYPILVPEIPKDDEIETAFRRSLDYKPTVRKIVGKGNNNQQKTPKLIKPTYISAKIENYDEIIELVKRCIASDAELTEKFKHLLASGKVQKELHITLGHVMSSREKEAKKLWKSYCNRYTDQITEYNNNRIENAQGSGNNQNTQVKTTDKLNFRLEKLCWDEKIIAIVVELSKDKDGCIIDENNEKIKGLCCQNKIPHITLCKLESGVKAVYSNVLCEKVESAEVDENIKVVKLDNSKEFVGSVYLNF
|
| Keyword |
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0255--Endonuclease
KW-0378--Hydrolase
KW-0418--Kinase
KW-0436--Ligase
KW-0511--Multifunctional enzyme
KW-0540--Nuclease
KW-0547--Nucleotide-binding
KW-1185--Reference proteome
KW-0808--Transferase
KW-0819--tRNA processing
|
| Interpro |
IPR027417--P-loop_NTPase
IPR019039--RNA_ligase_T4-Rnl1_N
IPR012387--Trl1_fun
IPR015966--tRNA_lig_kin_fun
IPR015965--tRNA_lig_PDEase_fun
|
| PROSITE |
|
| Pfam |
PF09511--RNA_lig_T4_1
PF08302--tRNA_lig_CPD
PF08303--tRNA_lig_kinase
|
| Gene Ontology |
GO:0005637--C:nuclear inner membrane
GO:0004113--F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity
GO:0005524--F:ATP binding
GO:0004519--F:endonuclease activity
GO:0051730--F:GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity
GO:0003972--F:RNA ligase (ATP) activity
GO:0030969--P:mRNA splicing via endonucleolytic cleavage and ligation involved in unfolded protein response
GO:0032056--P:positive regulation of translation in response to stress
GO:0006388--P:tRNA splicing, via endonucleolytic cleavage and ligation
|
