dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004865
Uniprot Accession P09064; IF2B_YEAST; D6W3D3; Q6B200;
Genbank Protein ID NP_015087.1;
Genbank Nucleotide ID NM_001184051.1;
Protein Name Eukaryotic translation initiation factor 2 subunit beta
Protein Synonyms/Alias
Gene Name SUI3
Gene Synonyms/Alias TIF212;YPL237W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Phosphorylation Sites
 dbPAF PTMs: 18
PositionPeptidesSourceReferences ( PMIDs )
2------MSSDLAAELdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;SysPTM 2.0;curated18407956; 16381945; 23193290; 21126336; 19823750; 19795423;
3-----MSSDLAAELGcurated26040406; 19547744
40VNGKENGSGDDLFAGdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 17563356; 18407956; 16381945; 23193290; 21177495;
55LKKKKKKSKSVSADAPhosphoPep 2.0;curated19060867; 26040289
57KKKKKSKSVSADAEAdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 19823750; 19779198; 19795423;
59KKKSKSVSADAEAEKdbPTM 3.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 17330950; 19366988; 26040406;
69AEAEKEPTDDIAEALdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19366988; 26040406;
80AEALGELSLKKKKKKdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19366988; 26040406;
88LKKKKKKTKDSSVDAPhosphoGRID 2.0;curated19779198; 22817900
91KKKKTKDSSVDAFEKdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21126336; 20702584; 19779198;
92KKKTKDSSVDAFEKEdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 20702584; 19779198;
112LDNVDAESKEGTPSAdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 17563356; 18407956; 16381945; 23193290; 21177495;
116DAESKEGTPSANSSIdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 17330950; 21177495;
118ESKEGTPSANSSIQQdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19060867; 19366988;
121EGTPSANSSIQQEVGPhosphoGRID 2.0;PhosphoPep 2.0;curated19779198; 19060867; 22817900
122GTPSANSSIQQEVGLcurated23607784
136LPYSELLSRFFNILRPhosphoGRID 2.019779198
251TELKREQSNRLFFMVdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17287358; 16381945; 23193290; 19823750; 19795423; 19060867;
Sequence
(Fasta)		MSSDLAAELG FDPALKKKKK TKKVIPDDFD AAVNGKENGS GDDLFAGLKK KKKKSKSVSA 60
DAEAEKEPTD DIAEALGELS LKKKKKKTKD SSVDAFEKEL AKAGLDNVDA ESKEGTPSAN 120
SSIQQEVGLP YSELLSRFFN ILRTNNPELA GDRSGPKFRI PPPVCLRDGK KTIFSNIQDI 180
AEKLHRSPEH LIQYLFAELG TSGSVDGQKR LVIKGKFQSK QMENVLRRYI LEYVTCKTCK 240
SINTELKREQ SNRLFFMVCK SCGSTRSVSS IKTGFQATVG KRRRM 286
Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0396--Initiation factor
KW-0479--Metal-binding
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
KW-0862--Zinc
KW-0863--Zinc-finger
Interpro

IPR002735--Transl_init_fac_IF2/IF5
IPR016189--Transl_init_fac_IF2/IF5_N
IPR016190--Transl_init_fac_IF2/IF5_Zn-bd
PROSITE
Pfam

PF01873--eIF-5_eIF-2B
Gene Ontology

GO:0016282--C:eukaryotic 43S preinitiation complex
GO:0005850--C:eukaryotic translation initiation factor 2 complex
GO:0043614--C:multi-eIF complex
GO:0046872--F:metal ion binding
GO:0003729--F:mRNA binding
GO:0003743--F:translation initiation factor activity
GO:0031369--F:translation initiation factor binding
GO:0001731--P:formation of translation preinitiation complex