dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004858
Uniprot Accession P09032; EI2BG_YEAST; D6W2W1; Q08721;
Genbank Protein ID NP_014903.1;
Genbank Nucleotide ID NM_001183679.1;
Protein Name Translation initiation factor eIF-2B subunit gamma
Protein Synonyms/Alias GCD complex subunit GCD1;Guanine nucleotide exchange factor subunit GCD1;eIF-2B GDP-GTP exchange factor subunit gamma;
Gene Name GCD1
Gene Synonyms/Alias TIF223;TRA3;YOR260W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Acts as essential component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD1 stabilizes the interaction between eIF-2 and GCD6 and stimulates the catalytic activity in vitro.
Phosphorylation Sites
 dbPAF PTMs: 10
PositionPeptidesSourceReferences ( PMIDs )
32FQTQNKDSTAATSGDdbPTM 3.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 26447709
36NKDSTAATSGDKLNEcurated22369663
37KDSTAATSGDKLNELdbPTM 3.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 24961812; 23825934;
294LLKLGPQSMSRQASFPhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;curated21177495; 21126336; 20702584; 19823750; 19795423; 21081558;
296KLGPQSMSRQASFKDdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15665377; 17287358; 18407956; 16381945; 23193290; 21177495;
300QSMSRQASFKDPFTGdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated17287358; 16381945; 23193290; 19366988; 22817900
306ASFKDPFTGNQQQQNPhosphoGRID 2.0;UniProt;curated19779198; 22817900
316QQQQNPPTTDDDEDRcurated22817900; 19823750; 25315811; 20377248
317QQQNPPTTDDDEDRNcurated26040289; 25315811; 20377248
336DDYKPSATSIQPTYFcurated19823750
Sequence
(Fasta)		MSIQAFVFCG KGSNLAPFTQ PDFPFQTQNK DSTAATSGDK LNELVNSALD STVINEFMQH 60
STRLPKALLP IGNRPMIEYV LDWCDQADFK EISVVAPVDE IELIESGLTS FLSLRKQQFE 120
LIYKALSNSN HSHHLQDPKK INFIPSKANS TGESLQKELL PRINGDFVIL PCDFVTDIPP 180
QVLVDQFRNR DDNNLAMTIY YKNSLDSSID KKQQQKQKQQ QFFTVYSENE DSERQPILLD 240
VYSQRDVTKT KYLQIRSHLL WNYPNLTVST KLLNSFIYFC SFELCQLLKL GPQSMSRQAS 300
FKDPFTGNQQ QQNPPTTDDD EDRNHDDDDD YKPSATSIQP TYFKKKNDLI LDPINCNKSL 360
SKVFRDLSRR SWQHSKPREP IGIFILPNET LFIRANNLNA YMDANRFVLK IKSQTMFTKN 420
IQIQSAAIGA DAIVDPKCQI SAHSNVKMSV LGTQANIGSR CRVAGSLLFP GVHLGDEVIL 480
ENCIIGPMAK IGSKCKLSNC YIEGHYVVEP KNNFKGETLA NVYLDEDEED ELIYDDSVIA 540
GESEIAEETD SDDRSDEDSD DSEYTDEYEY EDDGLFER 579
Keyword

KW-0181--Complete proteome
KW-0396--Initiation factor
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
KW-0810--Translation regulation
Interpro

IPR029044--Nucleotide-diphossugar_trans
IPR011004--Trimer_LpxA-like
PROSITE
Pfam
Gene Ontology

GO:0005851--C:eukaryotic translation initiation factor 2B complex
GO:0032045--C:guanyl-nucleotide exchange factor complex
GO:0003743--F:translation initiation factor activity
GO:1903574--P:negative regulation of cellular response to amino acid starvation
GO:0043547--P:positive regulation of GTPase activity
GO:0006446--P:regulation of translational initiation