dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004678
Uniprot Accession P08018; PBS2_YEAST; D6VW57;
Genbank Protein ID NP_012407.2;
Genbank Nucleotide ID NM_001181561.2;
Protein Name MAP kinase kinase PBS2
Protein Synonyms/Alias Polymyxin B resistance protein 2;Suppressor of fluoride sensitivity 4;
Gene Name PBS2
Gene Synonyms/Alias HOG4;SFS4;SSK4;YJL128C;J0699;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Seems to phosphorylate HOG1 on a tyrosine residue.
Phosphorylation Sites
 dbPAF PTMs: 37 (View all)
PositionPeptidesSourceReferences ( PMIDs )
9EDKFANLSLHEKTGKdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 25704821; 22369663;
14NLSLHEKTGKSSIQLcurated22369663; 24961812
17LHEKTGKSSIQLNEQcurated22369663
18HEKTGKSSIQLNEQTcurated22817900; 25315811; 20377248
27QLNEQTGSDNGSAVKcurated25315811
36NGSAVKRTSSTSSHYPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 19779198; 19060867; 22817900
37GSAVKRTSSTSSHYNPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 19060867; 22817900
38SAVKRTSSTSSHYNNdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17287358; 18407956; 16381945; 23193290; 17330950; 21177495;
39AVKRTSSTSSHYNNIPhosphoGRID 2.0;PhosphoPep 2.0;curated21177495; 19060867; 22817900; 19547744
40VKRTSSTSSHYNNINPhosphoGRID 2.0;PhosphoPep 2.0;curated19779198; 19060867; 26040289; 22817900; 24961812
41KRTSSTSSHYNNINAPhosphoPep 2.0;curated19060867; 22817900; 24961812
43TSSTSSHYNNINADLcurated22817900; 19547744
66EQRALKRSASVGSNQdbPTM 3.0;PhosphoGRID 2.0;curated17563356; 16381945; 23193290; 20702584; 22817900; 19823750;
68RALKRSASVGSNQSEdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17287358; 17563356; 16381945; 23193290; 20489023; 20702584;
71KRSASVGSNQSEQDKdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated11875433; 17563356; 16381945; 23193290; 19060867; 19366988;
74ASVGSNQSEQDKGSScurated22817900; 19823750; 25315811
80QSEQDKGSSQSPKHIcurated26447709
81SEQDKGSSQSPKHIQdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;curated17563356; 16381945; 23193290; 19060867; 22817900
83QDKGSSQSPKHIQQIdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.011875433; 16381945; 23193290; 19060867; 19366988
118SSQVVQASSKSTLKNcurated22369663
Sequence
(Fasta)		MEDKFANLSL HEKTGKSSIQ LNEQTGSDNG SAVKRTSSTS SHYNNINADL HARVKAFQEQ 60
RALKRSASVG SNQSEQDKGS SQSPKHIQQI VNKPLPPLPV AGSSKVSQRM SSQVVQASSK 120
STLKNVLDNQ ETQNITDVNI NIDTTKITAT TIGVNTGLPA TDITPSVSNT ASATHKAQLL 180
NPNRRAPRRP LSTQHPTRPN VAPHKAPAII NTPKQSLSAR RGLKLPPGGM SLKMPTKTAQ 240
QPQQFAPSPS NKKHIETLSN SKVVEGKRSN PGSLINGVQS TSTSSSTEGP HDTVGTTPRT 300
GNSNNSSNSG SSGGGGLFAN FSKYVDIKSG SLNFAGKLSL SSKGIDFSNG SSSRITLDEL 360
EFLDELGHGN YGNVSKVLHK PTNVIMATKE VRLELDEAKF RQILMELEVL HKCNSPYIVD 420
FYGAFFIEGA VYMCMEYMDG GSLDKIYDES SEIGGIDEPQ LAFIANAVIH GLKELKEQHN 480
IIHRDVKPTN ILCSANQGTV KLCDFGVSGN LVASLAKTNI GCQSYMAPER IKSLNPDRAT 540
YTVQSDIWSL GLSILEMALG RYPYPPETYD NIFSQLSAIV DGPPPRLPSD KFSSDAQDFV 600
SLCLQKIPER RPTYAALTEH PWLVKYRNQD VHMSEYITER LERRNKILRE RGENGLSKNV 660
PALHMGGL 669
Keyword

KW-0002--3D-structure
KW-0046--Antibiotic resistance
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0418--Kinase
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0808--Transferase
KW-0829--Tyrosine-protein kinase
Interpro

IPR011009--Kinase-like_dom
IPR000719--Prot_kinase_dom
IPR002290--Ser/Thr_dual-sp_kinase
IPR008271--Ser/Thr_kinase_AS
PROSITE

PS50011--PROTEIN_KINASE_DOM
PS00108--PROTEIN_KINASE_ST
Pfam

PF00069--Pkinase
Gene Ontology

GO:0005935--C:cellular bud neck
GO:0005934--C:cellular bud tip
GO:0005737--C:cytoplasm
GO:0005622--C:intracellular
GO:0031416--C:NatB complex
GO:0005524--F:ATP binding
GO:0004708--F:MAP kinase kinase activity
GO:0005078--F:MAP-kinase scaffold activity
GO:0004596--F:peptide alpha-N-acetyltransferase activity
GO:0004674--F:protein serine/threonine kinase activity
GO:0004713--F:protein tyrosine kinase activity
GO:0007015--P:actin filament organization
GO:0000169--P:activation of MAPK activity involved in osmosensory signaling pathway
GO:0071474--P:cellular hyperosmotic response
GO:0034605--P:cellular response to heat
GO:0006972--P:hyperosmotic response
GO:0000208--P:MAPK import into nucleus involved in osmosensory signaling pathway
GO:0017196--P:N-terminal peptidyl-methionine acetylation
GO:0007231--P:osmosensory signaling pathway
GO:0006468--P:protein phosphorylation
GO:0046677--P:response to antibiotic