dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004563
Uniprot Accession P07278; KAPR_YEAST; D6VVP9;
Genbank Protein ID NP_012231.1;
Genbank Nucleotide ID NM_001179383.1;
Protein Name cAMP-dependent protein kinase regulatory subunit
Protein Synonyms/Alias
Gene Name BCY1
Gene Synonyms/Alias REG1;SRA1;YIL033C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), an effector of the Ras/cAMP pathway. Inhibits PKA activity in the absence of cAMP. cAMP activates PKA and promotes growth and proliferation in response to good nutrient conditions. Together with ZDS1, provides a negative feedback control on the cell wall integrity-signaling pathway by acting as a negative regulator of MAP kinase SLT2/MPK1.
Phosphorylation Sites
 dbPAF PTMs: 25 (View all)
PositionPeptidesSourceReferences ( PMIDs )
3-----MVSSLPKESQdbPTM 3.0-; 16381945; 23193290
4----MVSSLPKESQAdbPTM 3.0-; 16381945; 23193290
9VSSLPKESQAELQLFdbPTM 3.0-; 16381945; 23193290
68LFPEPEESFSRPQSAdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 20702584; 16381945; 23193290; 21177495; 19060867;
70PEPEESFSRPQSAQSdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 20702584; 16381945; 23193290; 21177495; 19779198;
74ESFSRPQSAQSQSRSdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 20702584; 16381945; 23193290; 21177495;
77SRPQSAQSQSRSRSSdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 20702584; 16381945; 23193290; 21177495; 20489023;
79PQSAQSQSRSRSSVMdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated17563356; 20702584; 16381945; 23193290; 21177495; 19684113;
81SAQSQSRSRSSVMFKdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated-; 16381945; 23193290; 20702584; 19060867; 22369663; 2281790
83QSQSRSRSSVMFKSPdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated11875433; 17563356; 18407956; 20702584; 16381945; 23193290;
84SQSRSRSSVMFKSPFdbPTM 3.0;PhosphoGRID 2.0;UniProt;curated20702584; 16381945; 23193290; 21177495; 19779198; 26040406;
89RSSVMFKSPFVNEDPPhosphoGRID 2.0;PhosphoPep 2.0;curated19779198; 19060867; 24961812; 25315811
98FVNEDPHSNVFKSGFcurated22369663
114LDPHEQDTHQQAQEEcurated26040406
125AQEEQQHTREKTSTPcurated26040406
129QQHTREKTSTPPLPMdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 20702584; 16381945; 23193290; 17330950;
130QHTREKTSTPPLPMHdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 20702584; 16381945; 23193290; 21177495;
131HTREKTSTPPLPMHFdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 20702584; 16381945; 23193290; 17330950;
144HFNAQRRTSVSGETLdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17287358; 18407956; 20702584; 16381945; 23193290; 17330950;
145FNAQRRTSVSGETLQdbPTM 3.0;Phospho.ELM 9.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated3037314; 18407956; 20702584; 16381945; 23193290; 1956339; 17
Sequence
(Fasta)		MVSSLPKESQ AELQLFQNEI NAANPSDFLQ FSANYFNKRL EQQRAFLKAR EPEFKAKNIV 60
LFPEPEESFS RPQSAQSQSR SRSSVMFKSP FVNEDPHSNV FKSGFNLDPH EQDTHQQAQE 120
EQQHTREKTS TPPLPMHFNA QRRTSVSGET LQPNNFDDWT PDHYKEKSEQ QLQRLEKSIR 180
NNFLFNKLDS DSKRLVINCL EEKSVPKGAT IIKQGDQGDY FYVVEKGTVD FYVNDNKVNS 240
SGPGSSFGEL ALMYNSPRAA TVVATSDCLL WALDRLTFRK ILLGSSFKKR LMYDDLLKSM 300
PVLKSLTTYD RAKLADALDT KIYQPGETII REGDQGENFY LIEYGAVDVS KKGQGVINKL 360
KDHDYFGEVA LLNDLPRQAT VTATKRTKVA TLGKSGFQRL LGPAVDVLKL NDPTRH 417
Keyword

KW-0002--3D-structure
KW-0114--cAMP
KW-0116--cAMP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
Interpro

IPR012198--cAMP_dep_PK_reg_su
IPR003117--cAMP_dep_PK_reg_su_I/II_a/b
IPR018490--cNMP-bd-like
IPR018488--cNMP-bd_CS
IPR000595--cNMP-bd_dom
IPR014710--RmlC-like_jellyroll
PROSITE

PS00888--CNMP_BINDING_1
PS00889--CNMP_BINDING_2
PS50042--CNMP_BINDING_3
Pfam

PF00027--cNMP_binding
PF02197--RIIa
Gene Ontology

GO:0005952--C:cAMP-dependent protein kinase complex
GO:0005737--C:cytoplasm
GO:0000790--C:nuclear chromatin
GO:0005634--C:nucleus
GO:0005886--C:plasma membrane
GO:0030552--F:cAMP binding
GO:0004862--F:cAMP-dependent protein kinase inhibitor activity
GO:0008603--F:cAMP-dependent protein kinase regulator activity
GO:0042802--F:identical protein binding
GO:0071901--P:negative regulation of protein serine/threonine kinase activity
GO:0046580--P:negative regulation of Ras protein signal transduction
GO:0045762--P:positive regulation of adenylate cyclase activity
GO:0097271--P:protein localization to bud neck