dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004554
Uniprot Accession P07264; LEUC_YEAST; D6VUC8;
Genbank Protein ID NP_011506.1;
Genbank Nucleotide ID NM_001180874.1;
Protein Name 3-isopropylmalate dehydratase
Protein Synonyms/Alias Alpha-IPM isomerase;IPMI;Isopropylmalate isomerase;
Gene Name LEU1
Gene Synonyms/Alias YGL009C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.
Phosphorylation Sites
 dbPAF PTMs: 20
PositionPeptidesSourceReferences ( PMIDs )
4----MVYTPSKGPRTdbPTM 3.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 26040406; 26040289
44HLVHEVTSPQAFEGLdbPTM 3.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 19060867; 19366988; 26040406;
82ESRKNFKSLDTFIKQdbPTM 3.0;PhosphoGRID 2.0;curated17563356; 16381945; 23193290; 22817900
324VPDPKNVTDPIKKSGcurated26040289
345YMGLEPNTPLKSIKVPhosphoGRID 2.0;curated21126336; 26040289
361KVFIGSCTNGRIEDLdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
487EYKDQDQSSPKVEVTdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 21177495; 21126336; 20702584;
488YKDQDQSSPKVEVTSdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17287358; 17330950; 17563356; 18407956; 16381945; 23193290;
494SSPKVEVTSEDEKELdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated15665377; 18407956; 16381945; 23193290; 17330950; 21177495;
495SPKVEVTSEDEKELEdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17287358; 17563356; 18407956; 16381945; 23193290; 17330950;
503EDEKELESAAYDHAEdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 16381945; 23193290; 17330950; 21177495; 19779198;
506KELESAAYDHAEPVQdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17330950; 16381945; 23193290; 21177495; 19779198; 19060867;
533LKDIPVKSDDTPAKPcurated26040289; 22817900; 19823750; 23825934
536IPVKSDDTPAKPSSSdbPTM 3.0;PhosphoGRID 2.0;curated17563356; 16381945; 23193290; 22817900; 26040289; 25315811
541DDTPAKPSSSGMKPFcurated25315811
550SGMKPFLTLEGISAPcurated19823750
737LQKEEYISRYEALRRcurated24961812
748ALRREKYSFLEGGSKdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 21126336; 19366988;
769VPKRKAVTTTFDKVHPhosphoGRID 2.0;curated21126336; 26040406; 22817900; 25315811
771KRKAVTTTFDKVHQDcurated22817900; 19547744
Sequence
(Fasta)		MVYTPSKGPR TLYDKVFDAH VVHQDENGSF LLYIDRHLVH EVTSPQAFEG LENAGRKVRR 60
VDCTLATVDH NIPTESRKNF KSLDTFIKQT DSRLQVKTLE NNVKQFGVPY FGMSDARQGI 120
VHTIGPEEGF TLPGTTVVCG DSHTSTHGAF GSLAFGIGTS EVEHVLATQT IIQAKSKNMR 180
ITVNGKLSPG ITSKDLILYI IGLIGTAGGT GCVIEFAGEA IEALSMEARM SMCNMAIEAG 240
ARAGMIKPDE TTFQYTKGRP LAPKGAEWEK AVAYWKTLKT DEGAKFDHEI NIEAVDVIPT 300
ITWGTSPQDA LPITGSVPDP KNVTDPIKKS GMERALAYMG LEPNTPLKSI KVDKVFIGSC 360
TNGRIEDLRS AAAVVRGQKL ASNIKLAMVV PGSGLVKKQA EAEGLDKIFQ EAGFEWREAG 420
CSICLGMNPD ILDAYERCAS TSNRNFEGRQ GALSRTHLMS PAMAAAAGIA GHFVDIREFE 480
YKDQDQSSPK VEVTSEDEKE LESAAYDHAE PVQPEDAPQD IANDELKDIP VKSDDTPAKP 540
SSSGMKPFLT LEGISAPLDK ANVDTDAIIP KQFLKTIKRT GLKKGLFYEW RFRKDDQGKD 600
QETDFVLNVE PWREAEILVV TGDNFGCGSS REHAPWALKD FGIKSIIAPS YGDIFYNNSF 660
KNGLLPIRLD QQIIIDKLIP IANKGGKLCV DLPNQKILDS DGNVLVDHFE IEPFRKHCLV 720
NGLDDIGITL QKEEYISRYE ALRREKYSFL EGGSKLLKFD NVPKRKAVTT TFDKVHQDW 780
Keyword

KW-0004--4Fe-4S
KW-0028--Amino-acid biosynthesis
KW-0100--Branched-chain amino acid biosynthesis
KW-0181--Complete proteome
KW-0408--Iron
KW-0411--Iron-sulfur
KW-0432--Leucine biosynthesis
KW-0456--Lyase
KW-0479--Metal-binding
KW-0597--Phosphoprotein
KW-1185--Reference proteome
Interpro

IPR004430--3-IsopropMal_deHydase_lsu
IPR004431--3-IsopropMal_deHydase_ssu
IPR012235--3-IsopropMal_deHydtase_ssu/lsu
IPR015931--Acnase/IPM_dHydase_lsu_aba_1/3
IPR015937--Acoase/IPM_deHydtase
IPR001030--Acoase/IPM_deHydtase_lsu_aba
IPR015928--Aconitase/3IPM_dehydase_swvl
IPR015932--Aconitase/IPMdHydase_lsu_aba_2
IPR018136--Aconitase_4Fe-4S_BS
IPR000573--AconitaseA/IPMdHydase_ssu_swvl
PROSITE

PS00450--ACONITASE_1
PS01244--ACONITASE_2
Pfam

PF00330--Aconitase
PF00694--Aconitase_C
Gene Ontology

GO:0009316--C:3-isopropylmalate dehydratase complex
GO:0005829--C:cytosol
GO:0003861--F:3-isopropylmalate dehydratase activity
GO:0051539--F:4 iron, 4 sulfur cluster binding
GO:0046872--F:metal ion binding
GO:0009098--P:leucine biosynthetic process