dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004551
Uniprot Accession P07261; GCR1_YEAST; D6W3U1;
Genbank Protein ID NP_015250.1;
Genbank Nucleotide ID NM_001183889.1;
Protein Name Glycolytic genes transcriptional activator GCR1
Protein Synonyms/Alias
Gene Name GCR1
Gene Synonyms/Alias SIT3;YPL075W;LPF10W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Transcriptional activator required for the expression of glycolytic and ribosomal genes. Forms a transcriptional activation complex with RAP1, RAP1 providing the specific DNA-binding function and GCR1 providing the activation function. Can also bind itself to DNA to a core 5'-CTTCC-3' sequence (CT box). CT box-binding is not essential, but enhances the activation function of the RAP1-GCR1 complex in promoters that contain both DNA signals (only glycolytic genes). CT box-dependent transcriptional activation requires GCR2.
Phosphorylation Sites
 dbPAF PTMs: 19
PositionPeptidesSourceReferences ( PMIDs )
416RKLPLPGSIASAATGdbPTM 3.0;curated17563356; 16381945; 23193290; 22817900
422GSIASAATGSPFSPScurated24961812
424IASAATGSPFSPSPVdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 19366988; 22817900;
427AATGSPFSPSPVGEScurated24961812
429TGSPFSPSPVGESPYdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 19779198; 19366988;
434SPSPVGESPYSKRFKdbPTM 3.0;PhosphoGRID 2.0;curated17563356; 16381945; 23193290; 22817900; 24961812
447FKLDDKPTPSQTALDdbPTM 3.0;PhosphoGRID 2.0;UniProt;curated17563356; 16381945; 23193290; 19779198; 26040289; 22817900;
449LDDKPTPSQTALDSLdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated17563356; 18407956; 16381945; 23193290; 19366988; 26040406;
451DKPTPSQTALDSLLTcurated22369663; 25521595
455PSQTALDSLLTKSIScurated25521595
482AVTESFRSPQQFQHSUniProt;curated19779198; 26040289; 22817900; 25315811
489SPQQFQHSPDFVVGGdbPTM 3.0;PHOSIDA;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 21081558; 19366988; 19779198;
513SKKVNEDSPSSSSKLdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;curated17563356; 16381945; 23193290; 19060867; 24603354; 22817900;
515KVNEDSPSSSSKLAEPhosphoPep 2.019060867
516VNEDSPSSSSKLAERdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;curated17563356; 16381945; 23193290; 19060867; 22817900
518EDSPSSSSKLAERPRPhosphoPep 2.019060867
534PNNDSTTSMPESPTEcurated24961812
538STTSMPESPTEVAGDPhosphoGRID 2.0;UniProt;curated19779198; 26040289; 22817900; 24961812; 25315811
564KSEPNDNSPESKDPEdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988; 26040289; 25521595;
Sequence
(Fasta)		MVCTSTSSNF YSIAQYILQS YFKVNVDSLN SLKLVDLIVD QTYPDSLTLR KLNEGATGQP 60
YDYFNTVSRD ADISKCPIFA LTIFFVIRWS HPNPPISIEN FTTVPLLDSN FISLNSNPLL 120
YIQNQNPNSN SSVKVSRSQT FEPSKELIDL VFPWLSYLKQ DMLLIDRTNY KLYSLCELFE 180
FMGRVAIQDL RYLSQHPLLL PNIVTFISKF IPELFQNEEF KGIGSIKNSN NNALNNVTGI 240
ETQFLNPSTE EVSQKVDSYF MELSKKLTTE NIRLSQEITQ LKADMNSVGN VCNQILLLQR 300
QLLSGNQAIG SKSENIVSST GGGILILDKN SINSNVLSNL VQSIDPNHSK PNGQAQTHQR 360
GPKGQSHAQV QSTNSPALAP INMFPSLSNS IQPMLGTLAP QPQDIVQKRK LPLPGSIASA 420
ATGSPFSPSP VGESPYSKRF KLDDKPTPSQ TALDSLLTKS ISSPRLPLST LANTAVTESF 480
RSPQQFQHSP DFVVGGSSSS TTENNSKKVN EDSPSSSSKL AERPRLPNND STTSMPESPT 540
EVAGDDVDRE KPPESSKSEP NDNSPESKDP EKNGKNSNPL GTDADKPVPI SNIHNSTEAA 600
NSSGTVTKTA PSFPQSSSKF EIINKKDTKA GPNEAIKYKL SRENKTIWDL YAEWYIGLNG 660
KSSIKKLIEN YGWRRWKVSE DSHFFPTRRI IMDYIETECD RGIKLGRFTN PQQPREDIRK 720
ILVGDLEKFR INNGLTLNSL SLYFRNLTKN NKEICIFENF KNWNVRSMTE EEKLKYCKRR 780
HNTPS 786
Keyword

KW-0010--Activator
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0804--Transcription
KW-0805--Transcription regulation
Interpro

IPR022210--TF_GCR1-like
PROSITE
Pfam

PF12550--GCR1_C
Gene Ontology

GO:0005635--C:nuclear envelope
GO:0000978--F:RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0001077--F:transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0000433--P:negative regulation of transcription from RNA polymerase II promoter by glucose
GO:0034728--P:nucleosome organization
GO:0060963--P:positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter
GO:0061435--P:positive regulation of transcription from a mobile element promoter
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0072363--P:regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter
GO:0006366--P:transcription from RNA polymerase II promoter