dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004495
Uniprot Accession P06843; SPT2_YEAST; D3DM69;
Genbank Protein ID NP_011088.1;
Genbank Nucleotide ID NM_001179051.1;
Protein Name Protein SPT2
Protein Synonyms/Alias Negative regulator of Ty transcription;
Gene Name SPT2
Gene Synonyms/Alias SIN1;SPM2;YER161C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Histone chaperone that stabilizes pre-existing histone tetramers and regulates replication-independent histone exchange on chromatin (PubMed:26109053). Required for normal chromatin refolding in the coding region of transcribed genes, and for the suppression of spurious transcription (PubMed:26109053). Global regulatory protein that plays positive as well as negative regulatory roles in transcription (PubMed:2072912).
Phosphorylation Sites
 dbPAF PTMs: 7
PositionPeptidesSourceReferences ( PMIDs )
40YSLLPKNYIRDEDPAdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
126PEPIKKMSFEELMKQdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19823750; 19779198; 19795423;
146KQPPKVKSSEPVTKEcurated25315811
173KRPQKKASPGATLRGdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 21177495; 19823750;
183ATLRGVSSGGNSIKScurated25315811
187GVSSGGNSIKSSDSPcurated19823750
193NSIKSSDSPKPVKLNPhosphoPep 2.0;curated19060867; 25315811
Sequence
(Fasta)		MSFLSKLSQI RKSTTASKAQ VQDPLPKKND EEYSLLPKNY IRDEDPAVKR LKELRRQELL 60
KNGALAKKSG VKRKRGTSSG SEKKKIERND DDEGGLGIRF KRSIGASHAP LKPVVRKKPE 120
PIKKMSFEEL MKQAENNEKQ PPKVKSSEPV TKERPHFNKP GFKSSKRPQK KASPGATLRG 180
VSSGGNSIKS SDSPKPVKLN LPTNGFAQPN RRLKEKLESR KQKSRYQDDY DEEDNDMDDF 240
IEDDEDEGYH SKSKHSNGPG YDRDEIWAMF NRGKKRSEYD YDELEDDDME ANEMEILEEE 300
EMARKMARLE DKREEAWLKK HEEEKRRRKK GIR 334
Keyword

KW-0007--Acetylation
KW-0010--Activator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
Interpro

IPR013256--Chromatin_SPT2
PROSITE
Pfam

PF08243--SPT2
Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0003677--F:DNA binding
GO:0000217--F:DNA secondary structure binding
GO:0042393--F:histone binding
GO:0006325--P:chromatin organization
GO:0043486--P:histone exchange
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0010847--P:regulation of chromatin assembly
GO:0006355--P:regulation of transcription, DNA-templated
GO:0043631--P:RNA polyadenylation
GO:0006368--P:transcription elongation from RNA polymerase II promoter