| Tag |
Content |
| dbPAF ID |
dbPAF-0004494 |
| Uniprot Accession |
P06839; RAD3_YEAST; D3DM79;
|
| Genbank Protein ID |
NP_011098.3;
|
| Genbank Nucleotide ID |
NM_001179061.3;
|
| Protein Name |
DNA repair helicase RAD3 |
| Protein Synonyms/Alias |
General transcription and DNA repair factor IIH subunit RAD3;TFIIH subunit RAD3; |
| Gene Name |
RAD3 |
| Gene Synonyms/Alias |
REM1;YER171W; |
| Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast) |
| NCBI Taxa ID |
559292 |
Functional Description
(View all) |
ATP-dependent DNA helicase involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Necessary for excision of pyrimidine dimers. Also unwinds DNA/RNA duplexes. Plays an essential role in the cell viability. Involved in the maintenance of the fidelity of DNA replication. Acts as component of the general transcription and DNA repair factor IIH (TFIIH) core, which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro.Functional Description
ATP-dependent DNA helicase involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Necessary for excision of pyrimidine dimers. Also unwinds DNA/RNA duplexes. Plays an essential role in the cell viability. Involved in the maintenance of the fidelity of DNA replication. Acts as component of the general transcription and DNA repair factor IIH (TFIIH) core, which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro. |
Phosphorylation Sites
|
dbPAF PTMs: 1 | Position | Peptides | Source | References ( PMIDs ) |
|---|
| 482 | FKTVLQKSYAMTLAK | curated | 19823750 |
|
Sequence
(Fasta) | MKFYIDDLPV LFPYPKIYPE QYNYMCDIKK TLDVGGNSIL EMPSGTGKTV SLLSLTIAYQ 60
MHYPEHRKII YCSRTMSEIE KALVELENLM DYRTKELGYQ EDFRGLGLTS RKNLCLHPEV 120
SKERKGTVVD EKCRRMTNGQ AKRKLEEDPE ANVELCEYHE NLYNIEVEDY LPKGVFSFEK 180
LLKYCEEKTL CPYFIVRRMI SLCNIIIYSY HYLLDPKIAE RVSNEVSKDS IVIFDEAHNI 240
DNVCIESLSL DLTTDALRRA TRGANALDER ISEVRKVDSQ KLQDEYEKLV QGLHSADILT 300
DQEEPFVETP VLPQDLLTEA IPGNIRRAEH FVSFLKRLIE YLKTRMKVLH VISETPKSFL 360
QHLKQLTFIE RKPLRFCSER LSLLVRTLEV TEVEDFTALK DIATFATLIS TYEEGFLLII 420
EPYEIENAAV PNPIMRFTCL DASIAIKPVF ERFSSVIITS GTISPLDMYP RMLNFKTVLQ 480
KSYAMTLAKK SFLPMIITKG SDQVAISSRF EIRNDPSIVR NYGSMLVEFA KITPDGMVVF 540
FPSYLYMESI VSMWQTMGIL DEVWKHKLIL VETPDAQETS LALETYRKAC SNGRGAILLS 600
VARGKVSEGI DFDHQYGRTV LMIGIPFQYT ESRILKARLE FMRENYRIRE NDFLSFDAMR 660
HAAQCLGRVL RGKDDYGVMV LADRRFSRKR SQLPKWIAQG LSDADLNLST DMAISNTKQF 720
LRTMAQPTDP KDQEGVSVWS YEDLIKHQNS RKDQGGFIEN ENKEGEQDED EDEDIEMQ
779Fasta Sequence
>P06839|RAD3|Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
MKFYIDDLPVLFPYPKIYPEQYNYMCDIKKTLDVGGNSILEMPSGTGKTVSLLSLTIAYQMHYPEHRKIIYCSRTMSEIEKALVELENLMDYRTKELGYQEDFRGLGLTSRKNLCLHPEVSKERKGTVVDEKCRRMTNGQAKRKLEEDPEANVELCEYHENLYNIEVEDYLPKGVFSFEKLLKYCEEKTLCPYFIVRRMISLCNIIIYSYHYLLDPKIAERVSNEVSKDSIVIFDEAHNIDNVCIESLSLDLTTDALRRATRGANALDERISEVRKVDSQKLQDEYEKLVQGLHSADILTDQEEPFVETPVLPQDLLTEAIPGNIRRAEHFVSFLKRLIEYLKTRMKVLHVISETPKSFLQHLKQLTFIERKPLRFCSERLSLLVRTLEVTEVEDFTALKDIATFATLISTYEEGFLLIIEPYEIENAAVPNPIMRFTCLDASIAIKPVFERFSSVIITSGTISPLDMYPRMLNFKTVLQKSYAMTLAKKSFLPMIITKGSDQVAISSRFEIRNDPSIVRNYGSMLVEFAKITPDGMVVFFPSYLYMESIVSMWQTMGILDEVWKHKLILVETPDAQETSLALETYRKACSNGRGAILLSVARGKVSEGIDFDHQYGRTVLMIGIPFQYTESRILKARLEFMRENYRIRENDFLSFDAMRHAAQCLGRVLRGKDDYGVMVLADRRFSRKRSQLPKWIAQGLSDADLNLSTDMAISNTKQFLRTMAQPTDPKDQEGVSVWSYEDLIKHQNSRKDQGGFIENENKEGEQDEDEDEDIEMQ
|
| Keyword |
KW-0004--4Fe-4S
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0227--DNA damage
KW-0234--DNA repair
KW-0238--DNA-binding
KW-0347--Helicase
KW-0378--Hydrolase
KW-0408--Iron
KW-0411--Iron-sulfur
KW-0479--Metal-binding
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0804--Transcription
KW-0805--Transcription regulation
|
| Interpro |
IPR006555--ATP-dep_Helicase_C
IPR010614--DEAD_2
IPR002464--DNA/RNA_helicase_DEAH_CS
IPR013020--DNA_helicase_DNA-repair_Rad3
IPR010643--HBB
IPR014013--Helic_SF1/SF2_ATP-bd_DinG/Rad3
IPR006554--Helicase-like_DEXD_c2
IPR027417--P-loop_NTPase
IPR001945--XPGD_DNA_repair
|
| PROSITE |
PS00690--DEAH_ATP_HELICASE
PS51193--HELICASE_ATP_BIND_2
|
| Pfam |
PF06733--DEAD_2
PF06777--HBB
PF13307--Helicase_C_2
|
| Gene Ontology |
GO:0000439--C:core TFIIH complex
GO:0005829--C:cytosol
GO:0005675--C:holo TFIIH complex
GO:0000112--C:nucleotide-excision repair factor 3 complex
GO:0051539--F:4 iron, 4 sulfur cluster binding
GO:0043139--F:5'-3' DNA helicase activity
GO:0005524--F:ATP binding
GO:0043141--F:ATP-dependent 5'-3' DNA helicase activity
GO:0003684--F:damaged DNA binding
GO:0046872--F:metal ion binding
GO:0032508--P:DNA duplex unwinding
GO:0033683--P:nucleotide-excision repair, DNA incision
GO:0070816--P:phosphorylation of RNA polymerase II C-terminal domain
GO:0045951--P:positive regulation of mitotic recombination
GO:0000019--P:regulation of mitotic recombination
GO:0010525--P:regulation of transposition, RNA-mediated
GO:0006366--P:transcription from RNA polymerase II promoter
|
