dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004485
Uniprot Accession P06786; TOP2_YEAST; D6W191;
Genbank Protein ID NP_014311.3;
Genbank Nucleotide ID NM_001182926.3;
Protein Name DNA topoisomerase 2
Protein Synonyms/Alias DNA topoisomerase II;
Gene Name TOP2
Gene Synonyms/Alias TOR3;YNL088W;N2244;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.
Phosphorylation Sites
 dbPAF PTMs: 34 (View all)
PositionPeptidesSourceReferences ( PMIDs )
2------MSTEPVSAScurated26040406
3-----MSTEPVSASDcurated26040406
128FGHLLTSSNYDDDEKPhosphoGRID 2.019779198
130HLLTSSNYDDDEKKVPhosphoGRID 2.019779198
596EEESHKFTWKQKYYKcurated19823750
629RHLKIFHSLQGNDKDcurated25704821
1086INDVKGATSDEEDEEdbPTM 3.0;Phospho.ELM 9.0;PhosphoGRID 2.0;curated-; 16381945; 23193290; 1332607; 19779198; 22817900
1087NDVKGATSDEEDEESdbPTM 3.0;Phospho.ELM 9.0;PhosphoGRID 2.0;curated-; 16381945; 23193290; 1332607; 19779198; 22817900
1094SDEEDEESSHEDTENPhosphoGRID 2.019779198
1250TKIKKEKTPSVSETKdbPTM 3.0;PhosphoGRID 2.0;curated17563356; 16381945; 23193290; 19779198; 22817900; 24961812;
1252IKKEKTPSVSETKTEPhosphoGRID 2.0;UniProt;curated19779198; 22817900; 25315811
1254KEKTPSVSETKTEEEcurated25521595; 25315811
1256KTPSVSETKTEEEENcurated20377248
1258PSVSETKTEEEENAPdbPTM 3.0;Phospho.ELM 9.0-; 16381945; 23193290; 1332607
1266EEEENAPSSTSSSSIdbPTM 3.0;Phospho.ELM 9.0-; 16381945; 23193290; 1332607
1267EEENAPSSTSSSSIFcurated25315811
1268EENAPSSTSSSSIFDdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19366988
1269ENAPSSTSSSSIFDIdbPTM 3.0;Phospho.ELM 9.0;PhosphoGRID 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 1332607; 19366988
1272PSSTSSSSIFDIKKEdbPTM 3.0;Phospho.ELM 9.0-; 16381945; 23193290; 1332607
1290EGELSKISNKFKKISPhosphoGRID 2.0;curated19823750; 19795423; 22817900
Sequence
(Fasta)		MSTEPVSASD KYQKISQLEH ILKRPDTYIG SVETQEQLQW IYDEETDCMI EKNVTIVPGL 60
FKIFDEILVN AADNKVRDPS MKRIDVNIHA EEHTIEVKND GKGIPIEIHN KENIYIPEMI 120
FGHLLTSSNY DDDEKKVTGG RNGYGAKLCN IFSTEFILET ADLNVGQKYV QKWENNMSIC 180
HPPKITSYKK GPSYTKVTFK PDLTRFGMKE LDNDILGVMR RRVYDINGSV RDINVYLNGK 240
SLKIRNFKNY VELYLKSLEK KRQLDNGEDG AAKSDIPTIL YERINNRWEV AFAVSDISFQ 300
QISFVNSIAT TMGGTHVNYI TDQIVKKISE ILKKKKKKSV KSFQIKNNMF IFINCLIENP 360
AFTSQTKEQL TTRVKDFGSR CEIPLEYINK IMKTDLATRM FEIADANEEN ALKKSDGTRK 420
SRITNYPKLE DANKAGTKEG YKCTLVLTEG DSALSLAVAG LAVVGRDYYG CYPLRGKMLN 480
VREASADQIL KNAEIQAIKK IMGLQHRKKY EDTKSLRYGH LMIMTDQDHD GSHIKGLIIN 540
FLESSFPGLL DIQGFLLEFI TPIIKVSITK PTKNTIAFYN MPDYEKWREE ESHKFTWKQK 600
YYKGLGTSLA QEVREYFSNL DRHLKIFHSL QGNDKDYIDL AFSKKKADDR KEWLRQYEPG 660
TVLDPTLKEI PISDFINKEL ILFSLADNIR SIPNVLDGFK PGQRKVLYGC FKKNLKSELK 720
VAQLAPYVSE CTAYHHGEQS LAQTIIGLAQ NFVGSNNIYL LLPNGAFGTR ATGGKDAAAA 780
RYIYTELNKL TRKIFHPADD PLYKYIQEDE KTVEPEWYLP ILPMILVNGA EGIGTGWSTY 840
IPPFNPLEII KNIRHLMNDE ELEQMHPWFR GWTGTIEEIE PLRYRMYGRI EQIGDNVLEI 900
TELPARTWTS TIKEYLLLGL SGNDKIKPWI KDMEEQHDDN IKFIITLSPE EMAKTRKIGF 960
YERFKLISPI SLMNMVAFDP HGKIKKYNSV NEILSEFYYV RLEYYQKRKD HMSERLQWEV 1020
EKYSFQVKFI KMIIEKELTV TNKPRNAIIQ ELENLGFPRF NKEGKPYYGS PNDEIAEQIN 1080
DVKGATSDEE DEESSHEDTE NVINGPEELY GTYEYLLGMR IWSLTKERYQ KLLKQKQEKE 1140
TELENLLKLS AKDIWNTDLK AFEVGYQEFL QRDAEARGGN VPNKGSKTKG KGKRKLVDDE 1200
DYDPSKKNKK STARKGKKIK LEDKNFERIL LEQKLVTKSK APTKIKKEKT PSVSETKTEE 1260
EENAPSSTSS SSIFDIKKED KDEGELSKIS NKFKKISTIF DKMGSTSATS KENTPEQDDV 1320
ATKKNQTTAK KTAVKPKLAK KPVRKQQKVV ELSGESDLEI LDSYTDREDS NKDEDDAIPQ 1380
RSRRQRSSRA ASVPKKSYVE TLELSDDSFI EDDEEENQGS DVSFNEED 1429
Keyword

KW-0002--3D-structure
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-0413--Isomerase
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0799--Topoisomerase
Interpro

IPR024946--Arg_repress_C-like
IPR003594--HATPase_C
IPR020568--Ribosomal_S5_D2-typ_fold
IPR014721--Ribosomal_S5_D2-typ_fold_subgr
IPR001241--Topo_IIA
IPR013760--Topo_IIA-like_dom
IPR002205--Topo_IIA_A/C
IPR013758--Topo_IIA_A/C_ab
IPR013757--Topo_IIA_A_a
IPR013506--Topo_IIA_bsu_dom2
IPR013759--Topo_IIA_cen_dom
IPR018522--TopoIIA_CS
IPR031660--TOPRIM_C
IPR006171--Toprim_domain
PROSITE

PS00177--TOPOISOMERASE_II
PS50880--TOPRIM
Pfam

PF00204--DNA_gyraseB
PF00521--DNA_topoisoIV
PF02518--HATPase_c
PF01751--Toprim
PF16898--TOPRIM_C
Gene Ontology

GO:0097047--C:DNA replication termination region
GO:0009330--C:DNA topoisomerase complex (ATP-hydrolyzing)
GO:0005634--C:nucleus
GO:0000795--C:synaptonemal complex
GO:0005524--F:ATP binding
GO:0003677--F:DNA binding
GO:0003918--F:DNA topoisomerase type II (ATP-hydrolyzing) activity
GO:0046872--F:metal ion binding
GO:0006333--P:chromatin assembly or disassembly
GO:0031055--P:chromatin remodeling at centromere
GO:0006271--P:DNA strand elongation involved in DNA replication
GO:0006265--P:DNA topological change
GO:0006268--P:DNA unwinding involved in DNA replication
GO:0044774--P:mitotic DNA integrity checkpoint
GO:0007131--P:reciprocal meiotic recombination
GO:0000019--P:regulation of mitotic recombination
GO:0097046--P:replication fork progression beyond termination site
GO:0000712--P:resolution of meiotic recombination intermediates
GO:0000819--P:sister chromatid segregation