dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004444
Uniprot Accession P06634; DED1_YEAST; D6W2R0;
Genbank Protein ID NP_014847.3;
Genbank Nucleotide ID NM_001183623.3;
Protein Name ATP-dependent RNA helicase DED1
Protein Synonyms/Alias DEAD box protein 1;
Gene Name DED1
Gene Synonyms/Alias SPP81;YOR204W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 ATP-binding RNA helicase involved in translation initiation. Remodels RNA in response to ADP and ATP concentrations by facilitating disruption, but also formation of RNA duplexes. Has weak ATP-dependent affinity for dsRNA, but strong ATP-dependent affinity for ssRNA. Acts as a virus host factor involved in the replication of the MBV and the L-A viruses by promoting the negative-strand RNA synthesis. May be involved in recognition of the preinitiation complex and DNA binding of the RNA polymerase III and play a role in mRNA splicing.
Phosphorylation Sites
 dbPAF PTMs: 29 (View all)
PositionPeptidesSourceReferences ( PMIDs )
37PRSARNNSSNYNNNNcurated26040289; 22369663; 25315811
38RSARNNSSNYNNNNGcurated25315811
47YNNNNGGYNGGRGGGcurated22369663
55NGGRGGGSFFSNNRRPhosphoGRID 2.0;curated19779198; 22817900; 19547744
58RGGGSFFSNNRRGGYcurated22817900; 19547744
146EPITEFTSPPLDGLLcurated26040289
207VLSESFKTGPSPQPEPhosphoGRID 2.021126336
210ESFKTGPSPQPESQGdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19060867; 19366988; 20377248
215GPSPQPESQGSFYQRPhosphoGRID 2.0;UniProt;curated19779198; 22817900
218PQPESQGSFYQRKAYPhosphoGRID 2.0;UniProt;curated19779198; 22817900; 25315811
263ACVVYGGSPIGNQLRdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 21126336; 19779198; 19060867;
369LSVGRVGSTSENITQPhosphoGRID 2.0;curated21177495; 21126336; 19779198; 26040406; 22817900; 26040289;
436IHGDRTQSERERALAcurated26040406
451AFRSGAATLLVATAVPhosphoGRID 2.0;curated19779198; 22817900
456AATLLVATAVAARGLPhosphoGRID 2.0;curated19779198; 22817900
527EANQEVPSFLKDAMMcurated26040406
535FLKDAMMSAPGSRSNdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17287358; 17330950; 18407956; 16381945; 23193290; 21177495;
539AMMSAPGSRSNSRRGdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17287358; 17330950; 18407956; 16381945; 23193290; 21177495;
541MSAPGSRSNSRRGGFdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 21177495; 19684113;
543APGSRSNSRRGGFGRdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17287358; 18407956; 16381945; 23193290; 21177495; 19684113;
Sequence
(Fasta)		MAELSEQVQN LSINDNNENG YVPPHLRGKP RSARNNSSNY NNNNGGYNGG RGGGSFFSNN 60
RRGGYGNGGF FGGNNGGSRS NGRSGGRWID GKHVPAPRNE KAEIAIFGVP EDPNFQSSGI 120
NFDNYDDIPV DASGKDVPEP ITEFTSPPLD GLLLENIKLA RFTKPTPVQK YSVPIVANGR 180
DLMACAQTGS GKTGGFLFPV LSESFKTGPS PQPESQGSFY QRKAYPTAVI MAPTRELATQ 240
IFDEAKKFTY RSWVKACVVY GGSPIGNQLR EIERGCDLLV ATPGRLNDLL ERGKISLANV 300
KYLVLDEADR MLDMGFEPQI RHIVEDCDMT PVGERQTLMF SATFPADIQH LARDFLSDYI 360
FLSVGRVGST SENITQKVLY VENQDKKSAL LDLLSASTDG LTLIFVETKR MADQLTDFLI 420
MQNFRATAIH GDRTQSERER ALAAFRSGAA TLLVATAVAA RGLDIPNVTH VINYDLPSDV 480
DDYVHRIGRT GRAGNTGLAT AFFNSENSNI VKGLHEILTE ANQEVPSFLK DAMMSAPGSR 540
SNSRRGGFGR NNNRDYRKAG GASAGGWGSS RSRDNSFRGG SGWGSDSKSS GWGNSGGSNN 600
SSWW 605
Keyword

KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0347--Helicase
KW-0378--Hydrolase
KW-0396--Initiation factor
KW-0488--Methylation
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
KW-0694--RNA-binding
Interpro

IPR011545--DEAD/DEAH_box_helicase_dom
IPR014001--Helicase_ATP-bd
IPR001650--Helicase_C
IPR027417--P-loop_NTPase
IPR000629--RNA-helicase_DEAD-box_CS
IPR014014--RNA_helicase_DEAD_Q_motif
PROSITE

PS00039--DEAD_ATP_HELICASE
PS51192--HELICASE_ATP_BIND_1
PS51194--HELICASE_CTER
PS51195--Q_MOTIF
Pfam

PF00270--DEAD
PF00271--Helicase_C
Gene Ontology

GO:0005737--C:cytoplasm
GO:0036464--C:cytoplasmic ribonucleoprotein granule
GO:0005634--C:nucleus
GO:0005524--F:ATP binding
GO:0004004--F:ATP-dependent RNA helicase activity
GO:0033592--F:RNA strand annealing activity
GO:0003743--F:translation initiation factor activity
GO:0007059--P:chromosome segregation
GO:0010468--P:regulation of gene expression
GO:0010501--P:RNA secondary structure unwinding
GO:0000390--P:spliceosomal complex disassembly
GO:0006413--P:translational initiation