| Tag |
Content |
| dbPAF ID |
dbPAF-0004416 |
| Uniprot Accession |
P06242; KIN28_YEAST; D6VRP2;
|
| Genbank Protein ID |
NP_010175.1;
|
| Genbank Nucleotide ID |
NM_001180167.1;
|
| Protein Name |
Serine/threonine-protein kinase KIN28 |
| Protein Synonyms/Alias |
|
| Gene Name |
KIN28 |
| Gene Synonyms/Alias |
YDL108W;D2330; |
| Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast) |
| NCBI Taxa ID |
559292 |
Functional Description
(View all) |
Catalytic component of the TFIIK complex (KIN28-CCL1 dimer) which is the protein kinase component of transcription factor IIH (TFIIH) and phosphorylates the C-terminal domain of RNA polymerase II during transition from transcription to elongation after preinitiation complex (PIC) formation, thereby positively regulating transcription. TFIIH (or factor B) is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has DNA-dependent ATPase activity and is essential for polymerase II transcription in vitro. Essential for cell proliferation.Functional Description
Catalytic component of the TFIIK complex (KIN28-CCL1 dimer) which is the protein kinase component of transcription factor IIH (TFIIH) and phosphorylates the C-terminal domain of RNA polymerase II during transition from transcription to elongation after preinitiation complex (PIC) formation, thereby positively regulating transcription. TFIIH (or factor B) is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has DNA-dependent ATPase activity and is essential for polymerase II transcription in vitro. Essential for cell proliferation. |
Phosphorylation Sites
|
dbPAF PTMs: 4 |
Sequence
(Fasta) | MKVNMEYTKE KKVGEGTYAV VYLGCQHSTG RKIAIKEIKT SEFKDGLDMS AIREVKYLQE 60
MQHPNVIELI DIFMAYDNLN LVLEFLPTDL EVVIKDKSIL FTPADIKAWM LMTLRGVYHC 120
HRNFILHRDL KPNNLLFSPD GQIKVADFGL ARAIPAPHEI LTSNVVTRWY RAPELLFGAK 180
HYTSAIDIWS VGVIFAELML RIPYLPGQND VDQMEVTFRA LGTPTDRDWP EVSSFMTYNK 240
LQIYPPPSRD ELRKRFIAAS EYALDFMCGM LTMNPQKRWT AVQCLESDYF KELPPPSDPS 300
SIKIRN
307Fasta Sequence
>P06242|KIN28|Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
MKVNMEYTKEKKVGEGTYAVVYLGCQHSTGRKIAIKEIKTSEFKDGLDMSAIREVKYLQEMQHPNVIELIDIFMAYDNLNLVLEFLPTDLEVVIKDKSILFTPADIKAWMLMTLRGVYHCHRNFILHRDLKPNNLLFSPDGQIKVADFGLARAIPAPHEILTSNVVTRWYRAPELLFGAKHYTSAIDIWSVGVIFAELMLRIPYLPGQNDVDQMEVTFRALGTPTDRDWPEVSSFMTYNKLQIYPPPSRDELRKRFIAASEYALDFMCGMLTMNPQKRWTAVQCLESDYFKELPPPSDPSSIKIRN
|
| Keyword |
KW-0067--ATP-binding
KW-0131--Cell cycle
KW-0132--Cell division
KW-0181--Complete proteome
KW-0418--Kinase
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase
|
| Interpro |
IPR011009--Kinase-like_dom
IPR000719--Prot_kinase_dom
IPR017441--Protein_kinase_ATP_BS
IPR002290--Ser/Thr_dual-sp_kinase
IPR008271--Ser/Thr_kinase_AS
|
| PROSITE |
PS00107--PROTEIN_KINASE_ATP
PS50011--PROTEIN_KINASE_DOM
PS00108--PROTEIN_KINASE_ST
|
| Pfam |
PF00069--Pkinase
|
| Gene Ontology |
GO:0005829--C:cytosol
GO:0005675--C:holo TFIIH complex
GO:0005634--C:nucleus
GO:0070985--C:TFIIK complex
GO:0005524--F:ATP binding
GO:0008353--F:RNA polymerase II carboxy-terminal domain kinase activity
GO:0006370--P:7-methylguanosine mRNA capping
GO:0007049--P:cell cycle
GO:0051301--P:cell division
GO:0070816--P:phosphorylation of RNA polymerase II C-terminal domain
GO:1901921--P:phosphorylation of RNA polymerase II C-terminal domain involved in recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
GO:1900018--P:phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in recruitment of mRNA capping enzyme to RNA polymerase II holoenzyme complex
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0006360--P:transcription from RNA polymerase I promoter
GO:0006366--P:transcription from RNA polymerase II promoter
|
