dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0004408
Uniprot Accession P06169; PDC1_YEAST; D6VY46; O00042; Q07991; Q12682; Q12686; Q12687;
Genbank Protein ID NP_013145.1;
Genbank Nucleotide ID NM_001181931.1;
Protein Name Pyruvate decarboxylase isozyme 1
Protein Synonyms/Alias
Gene Name PDC1
Gene Synonyms/Alias YLR044C;L2104;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)
Major of three pyruvate decarboxylases (PDC1, PDC5, PDC6) implicated in the nonoxidative conversion of pyruvate to acetaldehyde and carbon dioxide during alcoholic fermentation. Most of the produced acetaldehyde is subsequently reduced to ethanol, but some is required for cytosolic acetyl-CoA production for biosynthetic pathways. The enzyme is also one of five 2-oxo acid decarboxylases (PDC1, PDC5, PDC6, ARO10, and THI3) able to decarboxylate more complex 2-oxo acids (alpha-ketoacids) than pyruvate, which seem mainly involved in amino acid catabolism. Here the enzyme catalyzes the decarboxylation of amino acids, which, in a first step, have been transaminated to the corresponding 2-oxo acids. In a third step, the resulting aldehydes are reduced to alcohols, collectively referred to as fusel oils or alcohols. Its preferred substrates are the transaminated amino acids valine, isoleucine, phenylalanine, and tryptophan, whereas leucine is no substrate. In a side-reaction the carbanionic intermediate (or active aldehyde) generated by decarboxylation or by activation of an aldehyde can react with an aldehyde via condensation (or carboligation) yielding a 2-hydroxy ketone, collectively called acyloins.
Phosphorylation Sites
dbPAF PTMs: 32 (View all)
PositionPeptidesSourceReferences ( PMIDs )
2------MSEITLGKYPhosphoGRID 2.0;curated19823750; 19795423
155EIDRCIRTTYVTQRPcurated25315811
183VPAKLLQTPIDMSLKcurated22369663; 24961812
188LQTPIDMSLKPNDAEcurated24961812
196LKPNDAESEKEVIDTPhosphoGRID 2.0;curated19823750; 19795423; 22369663; 22817900; 24961812; 25521595;
203SEKEVIDTILALVKDPhosphoGRID 2.0;curated19823750; 19795423; 22817900
223ILADACCSRHDVKAEdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 18407956; 16381945; 23193290; 19684113; 19823750;
238TKKLIDLTQFPAFVTPhosphoGRID 2.0;PhosphoPep 2.021126336; 19060867
245TQFPAFVTPMGKGSIcurated24961812; 26040289
259IDEQHPRYGGVYVGTPhosphoPep 2.019060867
263HPRYGGVYVGTLSKPPhosphoPep 2.019060867
266YGGVYVGTLSKPEVKdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19060867; 19366988; 22369663;
268GVYVGTLSKPEVKEAPhosphoPep 2.0;curated19060867; 24961812; 25315811; 20377248
294ALLSDFNTGSFSYSYPhosphoGRID 2.0;SysPTM 2.0;curated18407956; 19366988
296LSDFNTGSFSYSYKTPhosphoGRID 2.0;SysPTM 2.0;curated18407956; 19366988
300NTGSFSYSYKTKNIVSysPTM 2.0;curated18407956; 19366988
311KNIVEFHSDHMKIRNPhosphoPep 2.0;curated19060867; 22817900; 19547744
320HMKIRNATFPGVQMKdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated18407956; 16381945; 23193290; 19060867; 19366988
335FVLQKLLTTIADAAKPhosphoGRID 2.0;curated21126336; 19779198; 22817900; 24961812; 20377248
336VLQKLLTTIADAAKGPhosphoGRID 2.0;UniProt;curated19779198; 22817900; 20377248
Sequence
(Fasta)
MSEITLGKYL FERLKQVNVN TVFGLPGDFN LSLLDKIYEV EGMRWAGNAN ELNAAYAADG 60
YARIKGMSCI ITTFGVGELS ALNGIAGSYA EHVGVLHVVG VPSISAQAKQ LLLHHTLGNG 120
DFTVFHRMSA NISETTAMIT DIATAPAEID RCIRTTYVTQ RPVYLGLPAN LVDLNVPAKL 180
LQTPIDMSLK PNDAESEKEV IDTILALVKD AKNPVILADA CCSRHDVKAE TKKLIDLTQF 240
PAFVTPMGKG SIDEQHPRYG GVYVGTLSKP EVKEAVESAD LILSVGALLS DFNTGSFSYS 300
YKTKNIVEFH SDHMKIRNAT FPGVQMKFVL QKLLTTIADA AKGYKPVAVP ARTPANAAVP 360
ASTPLKQEWM WNQLGNFLQE GDVVIAETGT SAFGINQTTF PNNTYGISQV LWGSIGFTTG 420
ATLGAAFAAE EIDPKKRVIL FIGDGSLQLT VQEISTMIRW GLKPYLFVLN NDGYTIEKLI 480
HGPKAQYNEI QGWDHLSLLP TFGAKDYETH RVATTGEWDK LTQDKSFNDN SKIRMIEIML 540
PVFDAPQNLV EQAKLTAATN AKQ 564
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0021--Allosteric enzyme
KW-0101--Branched-chain amino acid catabolism
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0210--Decarboxylase
KW-0903--Direct protein sequencing
KW-0456--Lyase
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0585--Phenylalanine catabolism
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0786--Thiamine pyrophosphate
KW-0823--Tryptophan catabolism

Interpro

IPR029035--DHS-like_NAD/FAD-binding_dom
IPR029061--THDP-binding
IPR012000--Thiamin_PyroP_enz_cen_dom
IPR012001--Thiamin_PyroP_enz_TPP-bd_dom
IPR000399--TPP-bd_CS
IPR012110--TPP_enzyme
IPR011766--TPP_enzyme-bd_C

PROSITE

PS00187--TPP_ENZYMES

Pfam

PF02775--TPP_enzyme_C
PF00205--TPP_enzyme_M
PF02776--TPP_enzyme_N

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0047433--F:branched-chain-2-oxoacid decarboxylase activity
GO:0000287--F:magnesium ion binding
GO:0004737--F:pyruvate decarboxylase activity
GO:0030976--F:thiamine pyrophosphate binding
GO:0000949--P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway
GO:0009083--P:branched-chain amino acid catabolic process
GO:0019655--P:glycolytic fermentation to ethanol
GO:0006559--P:L-phenylalanine catabolic process
GO:0006090--P:pyruvate metabolic process
GO:0006569--P:tryptophan catabolic process