dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004397
Uniprot Accession P06102; NOT3_YEAST; D6VVP4; Q6B233;
Genbank Protein ID NP_012226.3;
Genbank Nucleotide ID NM_001179388.3;
Protein Name General negative regulator of transcription subunit 3
Protein Synonyms/Alias
Gene Name NOT3
Gene Synonyms/Alias YIL038C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.
Phosphorylation Sites
 dbPAF PTMs: 59 (View all)
PositionPeptidesSourceReferences ( PMIDs )
107EKAYSNISLKKSETLSysPTM 2.015665377; 19366988
111SNISLKKSETLDPQEPhosphoGRID 2.021126336
113ISLKKSETLDPQEREcurated26040406
257NAEDNNTSDANESLQdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
293KLAAKNATGAAIPVAPhosphoGRID 2.021126336
303AIPVAGPSSTPSPVIPhosphoGRID 2.0;PhosphoPep 2.0;UniProt;curated20702584; 19684113; 19779198; 19060867; 22369663; 22817900;
304IPVAGPSSTPSPVIPPhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;curated19684113; 21081558; 19060867; 26040289; 22817900; 20377248
305PVAGPSSTPSPVIPVPhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;curated20702584; 19779198; 21081558; 19060867; 19795423; 20377248
307AGPSSTPSPVIPVADPhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;UniProt;curated20702584; 19684113; 19779198; 21081558; 19060867; 26040289;
316VIPVADASKETERSPcurated26040289; 22369663; 22817900; 20377248
319VADASKETERSPSSSPhosphoGRID 2.0;PhosphoPep 2.0;curated19823750; 19795423; 19060867; 26040289; 22817900; 24961812;
322ASKETERSPSSSPIHdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17287358; 17563356; 16381945; 23193290; 21177495; 21081558;
324KETERSPSSSPIHNAdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;curated17563356; 16381945; 23193290; 21177495; 21081558; 19060867;
325ETERSPSSSPIHNATPhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;curated21177495; 19684113; 21081558; 19060867; 26040406; 25704821;
326TERSPSSSPIHNATKdbPTM 3.0;PhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;curated17287358; 17563356; 16381945; 23193290; 21177495; 19684113;
332SSPIHNATKPEEAVKPhosphoGRID 2.0;PHOSIDA;curated19779198; 21081558; 22817900; 19795423; 20377248
340KPEEAVKTSIKSPRScurated22817900; 19823750; 19547744
344AVKTSIKSPRSSADNdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17287358; 17563356; 18407956; 16381945; 23193290; 21177495;
347TSIKSPRSSADNLLPdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 19779198; 19366988;
348SIKSPRSSADNLLPSdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated17563356; 18407956; 16381945; 23193290; 19823750; 19779198;
Sequence
(Fasta)		MAHRKLQQEV DRVFKKINEG LEIFNSYYER HESCTNNPSQ KDKLESDLKR EVKKLQRLRE 60
QIKSWQSSPD IKDKDSLLDY RRSVEIAMEK YKAVEKASKE KAYSNISLKK SETLDPQERE 120
RRDISEYLSQ MIDELERQYD SLQVEIDKLL LLNKKKKTSS TTNDEKKEQY KRFQARYRWH 180
QQQMELALRL LANEELDPQD VKNVQDDINY FVESNQDPDF VEDETIYDGL NLQSNEAIAH 240
EVAQYFASQN AEDNNTSDAN ESLQDISKLS KKEQRKLERE AKKAAKLAAK NATGAAIPVA 300
GPSSTPSPVI PVADASKETE RSPSSSPIHN ATKPEEAVKT SIKSPRSSAD NLLPSLQKSP 360
SSATPETPTN VHTHIHQTPN GITGATTLKP ATLPAKPAGE LKWAVAASQA VEKDRKVTSA 420
SSTISNTSTK TPTTAAATTT SSNANSRIGS ALNTPKLSTS SLSLQPDNTG ASSSAATAAA 480
VLAAGAAAVH QNNQAFYRNM SSSHHPLVSL ATNPKSEHEV ATTVNQNGPE NTTKKVMEQK 540
EEESPEERNK LQVPTFGVFD DDFESDRDSE TEPEEEEQPS TPKYLSLEQR EAKTNEIKKE 600
FVSDFETLLL PSGVQEFIMS SELYNSQIES KITYKRSRDM CEISRLVEVP QGVNPPSPLD 660
AFRSTQQWDV MRCSLRDIII GSERLKEDSS SIYAKILENF RTLEMFSLFY NYYFAITPLE 720
REIAYKILNE RDWKVSKDGT MWFLRQGEVK FFNEICEVGD YKIFKLDDWT VIDKINFRLD 780
YSFLQPPVDT ASEVRDVSVD NNNVNDQSNV TLEQQKQEIS HGKQLLKQLK QGKISV 837
Keyword

KW-0010--Activator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
Interpro

IPR012270--CCR4-NOT_su3/5
IPR007282--NOT
IPR007207--Not_N
PROSITE
Pfam

PF04153--NOT2_3_5
PF04065--Not3
Gene Ontology

GO:0030015--C:CCR4-NOT core complex
GO:0005737--C:cytoplasm
GO:0000932--C:cytoplasmic mRNA processing body
GO:0005634--C:nucleus
GO:0017148--P:negative regulation of translation
GO:0000289--P:nuclear-transcribed mRNA poly(A) tail shortening
GO:0032968--P:positive regulation of transcription elongation from RNA polymerase II promoter
GO:0016567--P:protein ubiquitination
GO:0006357--P:regulation of transcription from RNA polymerase II promoter
GO:0006351--P:transcription, DNA-templated