dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004339
Uniprot Accession P05453; ERF3_YEAST; D6VSF4; P05420;
Genbank Protein ID NP_010457.3;
Genbank Nucleotide ID NM_001180479.3;
Protein Name Eukaryotic peptide chain release factor GTP-binding subunit
Protein Synonyms/Alias ERF-3;ERF3;ERF2;G1 to S phase transition protein 1;Omnipotent suppressor protein 2;PSI no more protein 2;Polypeptide release factor 3;Translation release factor 3;
Gene Name SUP35
Gene Synonyms/Alias GST1;PNM2;SAL3;SUF12;SUP2;YDR172W;YD9395.05;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Involved in translation termination. Stimulates the activity of ERF1. Binds guanine nucleotides. Recruited by polyadenylate-binding protein PAB1 to poly(A)-tails of mRNAs. Interaction with PAB1 is also required for regulation of normal mRNA decay through translation termination-coupled poly(A) shortening.
Phosphorylation Sites
 dbPAF PTMs: 13
PositionPeptidesSourceReferences ( PMIDs )
2------MSDSNQGNNcurated26040289; 22369663
148KKTLKLVSSSGIKLAPhosphoGRID 2.0;curated21126336; 19823750; 19795423; 25704821; 22369663; 22817900;
149KTLKLVSSSGIKLANcurated22369663; 19823750; 25315811
150TLKLVSSSGIKLANAPhosphoGRID 2.0;curated19823750; 19795423; 22369663; 22817900; 24961812; 25315811
221KVEDLKISESTHNTNdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
234TNNANVTSADALIKEcurated24961812; 25315811
282TMGGNLLYLTGSVDKcurated19823750
284GGNLLYLTGSVDKRTcurated19823750
286NLLYLTGSVDKRTIEcurated19823750
341ETEKRRYTILDAPGHcurated22817900; 19547744
570ISPGFVLTSPKNPIKcurated25521595
571SPGFVLTSPKNPIKSdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17330950; 17563356; 18407956; 16381945; 23193290; 19060867;
629EKGTNRKSKKPPAFAPhosphoGRID 2.0;curated19823750; 19795423; 22817900
Sequence
(Fasta)		MSDSNQGNNQ QNYQQYSQNG NQQQGNNRYQ GYQAYNAQAQ PAGGYYQNYQ GYSGYQQGGY 60
QQYNPDAGYQ QQYNPQGGYQ QYNPQGGYQQ QFNPQGGRGN YKNFNYNNNL QGYQAGFQPQ 120
SQGMSLNDFQ KQQKQAAPKP KKTLKLVSSS GIKLANATKK VGTKPAESDK KEEEKSAETK 180
EPTKEPTKVE EPVKKEEKPV QTEEKTEEKS ELPKVEDLKI SESTHNTNNA NVTSADALIK 240
EQEEEVDDEV VNDMFGGKDH VSLIFMGHVD AGKSTMGGNL LYLTGSVDKR TIEKYEREAK 300
DAGRQGWYLS WVMDTNKEER NDGKTIEVGK AYFETEKRRY TILDAPGHKM YVSEMIGGAS 360
QADVGVLVIS ARKGEYETGF ERGGQTREHA LLAKTQGVNK MVVVVNKMDD PTVNWSKERY 420
DQCVSNVSNF LRAIGYNIKT DVVFMPVSGY SGANLKDHVD PKECPWYTGP TLLEYLDTMN 480
HVDRHINAPF MLPIAAKMKD LGTIVEGKIE SGHIKKGQST LLMPNKTAVE IQNIYNETEN 540
EVDMAMCGEQ VKLRIKGVEE EDISPGFVLT SPKNPIKSVT KFVAQIAIVE LKSIIAAGFS 600
CVMHVHTAIE EVHIVKLLHK LEKGTNRKSK KPPAFAKKGM KVIAVLETEA PVCVETYQDY 660
PQLGRFTLRD QGTTIAIGKI VKIAE 686
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0034--Amyloid
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0342--GTP-binding
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0640--Prion
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
KW-0677--Repeat
Interpro

IPR031157--G_TR_CS
IPR027417--P-loop_NTPase
IPR000795--TF_GTP-bd_dom
IPR009000--Transl_B-barrel
IPR009001--Transl_elong_EF1A/Init_IF2_C
IPR004161--Transl_elong_EFTu/EF1A_2
IPR004160--Transl_elong_EFTu/EF1A_C
IPR003285--Yeast_ERF
PROSITE

PS00301--G_TR_1
PS51722--G_TR_2
Pfam

PF03144--GTP_EFTU_D2
PF03143--GTP_EFTU_D3
Gene Ontology

GO:0010494--C:cytoplasmic stress granule
GO:0005829--C:cytosol
GO:0018444--C:translation release factor complex
GO:0005525--F:GTP binding
GO:0003924--F:GTPase activity
GO:0003747--F:translation release factor activity
GO:0000288--P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
GO:0006415--P:translational termination