dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004182
Uniprot Accession P04386; GAL4_YEAST; D6W3C3;
Genbank Protein ID NP_015076.1;
Genbank Nucleotide ID NM_001184062.1;
Protein Name Regulatory protein GAL4
Protein Synonyms/Alias
Gene Name GAL4
Gene Synonyms/Alias YPL248C;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
Phosphorylation Sites
 dbPAF PTMs: 10
PositionPeptidesSourceReferences ( PMIDs )
132HRISATSSSEESSNKcurated22817900
136ATSSSEESSNKGQRQcurated22817900
691EENVNNISVKYVSPGPhosphoGRID 2.08756647
694VNNISVKYVSPGSVGPhosphoGRID 2.0;UniProt;curated19779198; 22817900
696NISVKYVSPGSVGPSPhosphoGRID 2.0;UniProt;curated19779198; 8756647; 22817900
699VKYVSPGSVGPSPVPPhosphoGRID 2.0;UniProt;curated19779198; 10360183; 10805731; 8756647; 15797387; 22817900
703SPGSVGPSPVPLKSGPhosphoGRID 2.0;UniProt;curated19779198; 22817900
709PSPVPLKSGASFSDLcurated22817900
712VPLKSGASFSDLVKLdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19366988; 22369663; 22817900;
837GNNSKPLSPGWTDQTPhosphoGRID 2.01961715; 10360183
Sequence
(Fasta)		MKLLSSIEQA CDICRLKKLK CSKEKPKCAK CLKNNWECRY SPKTKRSPLT RAHLTEVESR 60
LERLEQLFLL IFPREDLDMI LKMDSLQDIK ALLTGLFVQD NVNKDAVTDR LASVETDMPL 120
TLRQHRISAT SSSEESSNKG QRQLTVSIDS AAHHDNSTIP LDFMPRDALH GFDWSEEDDM 180
SDGLPFLKTD PNNNGFFGDG SLLCILRSIG FKPENYTNSN VNRLPTMITD RYTLASRSTT 240
SRLLQSYLNN FHPYCPIVHS PTLMMLYNNQ IEIASKDQWQ ILFNCILAIG AWCIEGESTD 300
IDVFYYQNAK SHLTSKVFES GSIILVTALH LLSRYTQWRQ KTNTSYNFHS FSIRMAISLG 360
LNRDLPSSFS DSSILEQRRR IWWSVYSWEI QLSLLYGRSI QLSQNTISFP SSVDDVQRTT 420
TGPTIYHGII ETARLLQVFT KIYELDKTVT AEKSPICAKK CLMICNEIEE VSRQAPKFLQ 480
MDISTTALTN LLKEHPWLSF TRFELKWKQL SLIIYVLRDF FTNFTQKKSQ LEQDQNDHQS 540
YEVKRCSIML SDAAQRTVMS VSSYMDNHNV TPYFAWNCSY YLFNAVLVPI KTLLSNSKSN 600
AENNETAQLL QQINTVLMLL KKLATFKIQT CEKYIQVLEE VCAPFLLSQC AIPLPHISYN 660
NSNGSAIKNI VGSATIAQYP TLPEENVNNI SVKYVSPGSV GPSPVPLKSG ASFSDLVKLL 720
SNRPPSRNSP VTIPRSTPSH RSVTPFLGQQ QQLQSLVPLT PSALFGGANF NQSGNIADSS 780
LSFTFTNSSN GPNLITTQTN SQALSQPIAS SNVHDNFMNN EITASKIDDG NNSKPLSPGW 840
TDQTAYNAFG ITTGMFNTTT MDDVYNYLFD DEDTPPNPKK E 882
Keyword

KW-0002--3D-structure
KW-0010--Activator
KW-0119--Carbohydrate metabolism
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-0299--Galactose metabolism
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0862--Zinc
Interpro

IPR005600--Gal4_dimer_dom
IPR007219--Transcription_factor_dom_fun
IPR001138--Zn2-C6_fun-type_DNA-bd
PROSITE

PS00463--ZN2_CY6_FUNGAL_1
PS50048--ZN2_CY6_FUNGAL_2
Pfam

PF04082--Fungal_trans
PF03902--Gal4_dimer
PF00172--Zn_clus
Gene Ontology

GO:0005634--C:nucleus
GO:0000978--F:RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0001133--F:RNA polymerase II transcription factor activity, sequence-specific transcription regulatory region DNA binding
GO:0001085--F:RNA polymerase II transcription factor binding
GO:0001077--F:transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0008270--F:zinc ion binding
GO:0006012--P:galactose metabolic process
GO:0000435--P:positive regulation of transcription from RNA polymerase II promoter by galactose
GO:0006366--P:transcription from RNA polymerase II promoter