dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004147
Uniprot Accession P04173; LEU3_YEAST; D6VQZ6;
Genbank Protein ID NP_009911.2;
Genbank Nucleotide ID NM_001178665.1;
Protein Name 3-isopropylmalate dehydrogenase
Protein Synonyms/Alias
Gene Name LEU2
Gene Synonyms/Alias YCL018W;YCL18W;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Phosphorylation Sites
 dbPAF PTMs: 5
PositionPeptidesSourceReferences ( PMIDs )
54GGAAIDATGVPLPDEcurated22817900
66PDEALEASKKADAVLcurated22817900
84VGGPKWGTGSVRPEQPhosphoPep 2.019060867
226VQHQLIDSAAMILVKPhosphoPep 2.019060867
290LYEPCHGSAPDLPKNdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
Sequence
(Fasta)		MSAPKKIVVL PGDHVGQEIT AEAIKVLKAI SDVRSNVKFD FENHLIGGAA IDATGVPLPD 60
EALEASKKAD AVLLGAVGGP KWGTGSVRPE QGLLKIRKEL QLYANLRPCN FASDSLLDLS 120
PIKPQFAKGT DFVVVRELVG GIYFGKRKED DGDGVAWDSE QYTVPEVQRI TRMAAFMALQ 180
HEPPLPIWSL DKANVLASSR LWRKTVEETI KNEFPTLKVQ HQLIDSAAMI LVKNPTHLNG 240
IIITSNMFGD IISDEASVIP GSLGLLPSAS LASLPDKNTA FGLYEPCHGS APDLPKNKVN 300
PIATILSAAM MLKLSLNLPE EGKAIEDAVK KVLDAGIRTG DLGGSNSTTE VGDAVAEEVK 360
KILA 365
Keyword

KW-0028--Amino-acid biosynthesis
KW-0100--Branched-chain amino acid biosynthesis
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0432--Leucine biosynthesis
KW-0460--Magnesium
KW-0464--Manganese
KW-0479--Metal-binding
KW-0520--NAD
KW-0560--Oxidoreductase
KW-1185--Reference proteome
Interpro

IPR019818--IsoCit/isopropylmalate_DH_CS
IPR024084--IsoPropMal-DH-like_dom
IPR004429--Isopropylmalate_DH
PROSITE

PS00470--IDH_IMDH
Pfam

PF00180--Iso_dh
Gene Ontology

GO:0005829--C:cytosol
GO:0003862--F:3-isopropylmalate dehydrogenase activity
GO:0000287--F:magnesium ion binding
GO:0051287--F:NAD binding
GO:0006097--P:glyoxylate cycle
GO:0009098--P:leucine biosynthetic process