dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004085
Uniprot Accession P03877; SCE3_YEAST; A0A0A7P044; Q35790; Q9ZZX3;
Genbank Protein ID NP_009308.2;
Genbank Nucleotide ID NC_001224.1;
Protein Name Intron-encoded DNA endonuclease aI3
Protein Synonyms/Alias DNA endonuclease I-SceIII;
Gene Name AI3
Gene Synonyms/Alias ENS3;I-SCEIII;Q0060;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 Mitochondrial DNA endonuclease involved in intron homing. It introduces a specific double-strand break in the DNA of the COX1 gene and thus mediates the insertion of an intron, containing its own coding sequence (group I intron), into an intronless gene. Recognizes with high specificity and cleaves the sequence 5'-GGTTTTGGTAACTATTTATTAC-3'.
Phosphorylation Sites
 dbPAF PTMs: 2
PositionPeptidesSourceReferences ( PMIDs )
172YKKINRNYVLWTIHDcurated23825934
176NRNYVLWTIHDLKGVcurated23825934
Sequence
(Fasta)		MVQRWLYSTN AKDIAVLYFM LAIFSGMAGT AMSLIIRLEL AAPGSQYLHG NSQLFNVLVV 60
GHAVLMIFFL VMPALIGGFG NQKRYESNNN NNQVMENKEY NLKLNYDKLG PYLAGLIEGD 120
GTITVQNSSS MKKSKYRPLI VVVFKLEDLE LANYLCNLTK CGKVYKKINR NYVLWTIHDL 180
KGVYTLLNII NGYMRTPKYE AFVRGAEFMN NYINSTTITH NKLKNMDNIK IKPLDTSDIG 240
SNAWLAGMTD ADGNFSINLM NGKNRSSRAM PYYCLELRQN YQKNSNNNNI NFSYFYIMSA 300
IATYFNVNLY SRERNLNLLV STNNTYKTYY SYKVMVANTY KNIKVMEYFN KYSLLSSKHL 360
DFLDWSKLVI LINNEGQSMK TNGSWELGMN LRKDYNKTRT TFTWSHLKNT YLENK 416
Keyword

KW-0181--Complete proteome
KW-0255--Endonuclease
KW-0378--Hydrolase
KW-0404--Intron homing
KW-0472--Membrane
KW-0496--Mitochondrion
KW-0507--mRNA processing
KW-0508--mRNA splicing
KW-0540--Nuclease
KW-1185--Reference proteome
KW-0812--Transmembrane
KW-1133--Transmembrane helix
Interpro

IPR000883--COX1
IPR023616--Cyt_c_Oxase_su1_dom
IPR027434--Homing_endonucl
IPR004860--LAGLIDADG_2
PROSITE

PS50855--COX1
Pfam

PF00115--COX1
PF00961--LAGLIDADG_1
Gene Ontology

GO:0016021--C:integral component of membrane
GO:0005739--C:mitochondrion
GO:0004129--F:cytochrome-c oxidase activity
GO:0004519--F:endonuclease activity
GO:0020037--F:heme binding
GO:0005506--F:iron ion binding
GO:0009060--P:aerobic respiration
GO:0006314--P:intron homing
GO:0006397--P:mRNA processing
GO:0090305--P:nucleic acid phosphodiester bond hydrolysis
GO:0008380--P:RNA splicing