dbPAF Protein Information
| Tag | Content |
|---|---|
| dbPAF ID | dbPAF-0004082 |
| Uniprot Accession | P03872; REP2_YEAST; |
| Genbank Protein ID | |
| Genbank Nucleotide ID | |
| Protein Name | Partitioning protein REP2 |
| Protein Synonyms/Alias | |
| Gene Name | REP2 |
| Gene Synonyms/Alias | R0040C; |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast) |
| NCBI Taxa ID | 559292 |
| Functional Description (View all) |
Part of the plasmid partitioning system, which ensures the equal distribution of replicated plasmid molecules to daugther cells. The plasmids exist as well-organized plasmid foci within the nucleus that stay together throughout the cell-cycle and act as entity during segregation, effetively reducing copy number to one. Plasmid partitioning requires the proteins REP1, REP2, and a cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-containing chromatin at STB during S-phase, marking the locus with a centromeric tag, and thereby probably catching mitotic spindle microtubules to the plasmid cluster and coupling plasmid segregation to chromosome segregation. REP1-REP2 are required to recruit the cohesin complex to the STB locus for pairing of the replicated plasmid cluster, a prerequisite for successful plasmid segregation. REP1-REP2 also negatively regulate expression of site-specific recombinase FLP and of RAF1. |
| Phosphorylation Sites |
dbPAF PTMs: 4 |
| Sequence (Fasta) | MDDIETAKNL TVKARTAYSV WDVCRLFIEM IAPDVDIDIE SKRKSDELLF PGYVIRPMES 60 LTTGRPYGLD SSAEDSSVSS DSSAEVILPA AKMVKERFDS IGNGMLSSQE ASQAAIDLML 120 QNNKLLDNRK QLYKSIAIII GRLPEKDKKR ATEMLMRKMD CTQLLVPPAP TEEDVMKLVS 180 VVTQLLTLVP PDRQAALIGD LFIPESLKDI FNSFNELAAE NRLQQKKSEL EGRTEVNHAN 240 TNEEVPSRRT RSRDTNARGA YKLQNTITEG PKAVPTKKRR VATRVRGRKS RNTSRV 297 |
| Keyword | KW-0007--Acetylation |
| Interpro | |
| PROSITE | |
| Pfam | |
| Gene Ontology | GO:0005634--C:nucleus |