dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0004078
Uniprot Accession P02994; EF1A_YEAST; D6VQB6; Q7Z7U8; Q7Z8Q8; Q7Z8Q9;
Genbank Protein ID NP_009676.1; NP_015405.1;
Genbank Nucleotide ID NM_001178466.1; NM_001184177.1;
Protein Name Elongation factor 1-alpha
Protein Synonyms/Alias
Gene Name TEF2
Gene Synonyms/Alias YPR080W;P9513.7;YBR118W;YBR0913;
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast)
NCBI Taxa ID 559292
Functional Description
(View all)		 GTP-binding component of the eukaryotic elongation factor 1 complex (eEF1). In its active GTP-bound form, binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In the presence of a correct codon-anticodon match between the aminoacyl-tRNA and the A-site codon of the ribosome-bound mRNA, the ribosome acts as a GTPase activator and the GTP is hydrolyzed. The inactive GDP-bound form leaves the ribosome and must be recycled by its guanine nucleotide exchange factor (GEF) (eEF1B subcomplex) before binding another molecule of aminoacyl-tRNA. Required for nuclear export of aminoacyl-tRNAs. May also be involved in translational quality control by targeting cotranslationally damaged proteins to the proteasome. Also exhibits actin filament-binding and -bundling activities and is involved in cytoskeleton organization.
Phosphorylation Sites
 dbPAF PTMs: 22 (View all)
PositionPeptidesSourceReferences ( PMIDs )
6--MGKEKSHINVVVIdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;curated17287358; 16381945; 23193290; 19366988; 26040289; 24961812
18VVIGHVDSGKSTTTGdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated17287358; 16381945; 23193290; 19366988; 22817900
22HVDSGKSTTTGHLIYcurated20377248
53AAELGKGSFKYAWVLdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
72AERERGITIDIALWKdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated17287358; 16381945; 23193290; 19366988
82IALWKFETPKYQVTVPhosphoGRID 2.0;SysPTM 2.0;UniProt;curated17287358; 19366988
157VAVNKMDSVKWDESRcurated22817900; 20377248
163DSVKWDESRFQEIVKdbPTM 3.0;PhosphoGRID 2.0;SysPTM 2.0;UniProt;curated17287358; 16381945; 23193290; 19366988; 22369663; 22817900;
237IDAIEQPSRPTDKPLPhosphoPep 2.0;curated19060867; 22817900
259YKIGGIGTVPVGRVEdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18407956; 16381945; 23193290; 19779198; 19060867; 19366988;
267VPVGRVETGVIKPGMcurated20377248
284TFAPAGVTTEVKSVEPhosphoPep 2.0;curated19060867; 20377248
285FAPAGVTTEVKSVEMPhosphoPep 2.019060867
289GVTTEVKSVEMHHEQPhosphoGRID 2.0;PHOSIDA;PhosphoPep 2.0;UniProt;curated19823750; 19779198; 19795423; 21081558; 19060867; 26040289;
355PGQISAGYSPVLDCHdbPTM 3.0;SysPTM 2.018407956; 16381945; 23193290; 19366988
356GQISAGYSPVLDCHTdbPTM 3.0;PhosphoPep 2.0;SysPTM 2.018407956; 16381945; 23193290; 19060867; 19366988
394DHPKFLKSGDAALVKdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;curated15665377; 18407956; 16381945; 23193290; 19060867; 19366988;
405ALVKFVPSKPMCVEAPhosphoPep 2.019060867
414PMCVEAFSEYPPLGRdbPTM 3.0;PhosphoGRID 2.0;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated17287358; 18407956; 16381945; 23193290; 19060867; 19366988;
416CVEAFSEYPPLGRFAPhosphoPep 2.019060867
Sequence
(Fasta)		MGKEKSHINV VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAELG KGSFKYAWVL 60
DKLKAERERG ITIDIALWKF ETPKYQVTVI DAPGHRDFIK NMITGTSQAD CAILIIAGGV 120
GEFEAGISKD GQTREHALLA FTLGVRQLIV AVNKMDSVKW DESRFQEIVK ETSNFIKKVG 180
YNPKTVPFVP ISGWNGDNMI EATTNAPWYK GWEKETKAGV VKGKTLLEAI DAIEQPSRPT 240
DKPLRLPLQD VYKIGGIGTV PVGRVETGVI KPGMVVTFAP AGVTTEVKSV EMHHEQLEQG 300
VPGDNVGFNV KNVSVKEIRR GNVCGDAKND PPKGCASFNA TVIVLNHPGQ ISAGYSPVLD 360
CHTAHIACRF DELLEKNDRR SGKKLEDHPK FLKSGDAALV KFVPSKPMCV EAFSEYPPLG 420
RFAVRDMRQT VAVGVIKSVD KTEKAAKVTK AAQKAAKK 459
Keyword

KW-0002--3D-structure
KW-0009--Actin-binding
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0903--Direct protein sequencing
KW-0251--Elongation factor
KW-0342--GTP-binding
KW-0488--Methylation
KW-0547--Nucleotide-binding
KW-0597--Phosphoprotein
KW-0648--Protein biosynthesis
KW-1185--Reference proteome
Interpro

IPR031157--G_TR_CS
IPR027417--P-loop_NTPase
IPR000795--TF_GTP-bd_dom
IPR009000--Transl_B-barrel
IPR009001--Transl_elong_EF1A/Init_IF2_C
IPR004539--Transl_elong_EF1A_euk/arc
IPR004161--Transl_elong_EFTu/EF1A_2
IPR004160--Transl_elong_EFTu/EF1A_C
PROSITE

PS00301--G_TR_1
PS51722--G_TR_2
Pfam

PF03144--GTP_EFTU_D2
PF03143--GTP_EFTU_D3
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005856--C:cytoskeleton
GO:0005853--C:eukaryotic translation elongation factor 1 complex
GO:0000329--C:fungal-type vacuole membrane
GO:0005739--C:mitochondrion
GO:0005840--C:ribosome
GO:0019003--F:GDP binding
GO:0005525--F:GTP binding
GO:0003924--F:GTPase activity
GO:0003746--F:translation elongation factor activity
GO:0006414--P:translational elongation
GO:0006409--P:tRNA export from nucleus