Tag |
Content |
dbPAF ID |
dbPAF-0004078 |
Uniprot Accession |
P02994; EF1A_YEAST; D6VQB6; Q7Z7U8; Q7Z8Q8; Q7Z8Q9; |
Genbank Protein ID |
NP_009676.1; NP_015405.1; |
Genbank Nucleotide ID |
NM_001178466.1; NM_001184177.1; |
Protein Name |
Elongation factor 1-alpha |
Protein Synonyms/Alias |
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Gene Name |
TEF2 |
Gene Synonyms/Alias |
YPR080W;P9513.7;YBR118W;YBR0913; |
Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast) |
NCBI Taxa ID |
559292 |
Functional Description (View all) |
GTP-binding component of the eukaryotic elongation factor 1 complex (eEF1). In its active GTP-bound form, binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In the presence of a correct codon-anticodon match between the aminoacyl-tRNA and the A-site codon of the ribosome-bound mRNA, the ribosome acts as a GTPase activator and the GTP is hydrolyzed. The inactive GDP-bound form leaves the ribosome and must be recycled by its guanine nucleotide exchange factor (GEF) (eEF1B subcomplex) before binding another molecule of aminoacyl-tRNA. Required for nuclear export of aminoacyl-tRNAs. May also be involved in translational quality control by targeting cotranslationally damaged proteins to the proteasome. Also exhibits actin filament-binding and -bundling activities and is involved in cytoskeleton organization.Functional Description
GTP-binding component of the eukaryotic elongation factor 1 complex (eEF1). In its active GTP-bound form, binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In the presence of a correct codon-anticodon match between the aminoacyl-tRNA and the A-site codon of the ribosome-bound mRNA, the ribosome acts as a GTPase activator and the GTP is hydrolyzed. The inactive GDP-bound form leaves the ribosome and must be recycled by its guanine nucleotide exchange factor (GEF) (eEF1B subcomplex) before binding another molecule of aminoacyl-tRNA. Required for nuclear export of aminoacyl-tRNAs. May also be involved in translational quality control by targeting cotranslationally damaged proteins to the proteasome. Also exhibits actin filament-binding and -bundling activities and is involved in cytoskeleton organization.
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Phosphorylation Sites
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dbPAF PTMs: 22 (View all) |
Sequence (Fasta) | MGKEKSHINV VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAELG KGSFKYAWVL 60 DKLKAERERG ITIDIALWKF ETPKYQVTVI DAPGHRDFIK NMITGTSQAD CAILIIAGGV 120 GEFEAGISKD GQTREHALLA FTLGVRQLIV AVNKMDSVKW DESRFQEIVK ETSNFIKKVG 180 YNPKTVPFVP ISGWNGDNMI EATTNAPWYK GWEKETKAGV VKGKTLLEAI DAIEQPSRPT 240 DKPLRLPLQD VYKIGGIGTV PVGRVETGVI KPGMVVTFAP AGVTTEVKSV EMHHEQLEQG 300 VPGDNVGFNV KNVSVKEIRR GNVCGDAKND PPKGCASFNA TVIVLNHPGQ ISAGYSPVLD 360 CHTAHIACRF DELLEKNDRR SGKKLEDHPK FLKSGDAALV KFVPSKPMCV EAFSEYPPLG 420 RFAVRDMRQT VAVGVIKSVD KTEKAAKVTK AAQKAAKK
459Fasta Sequence
>P02994|TEF2|Saccharomyces cerevisiae (strain ATCC 204508 / S288c)(Baker's yeast) MGKEKSHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYQVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAFTLGVRQLIVAVNKMDSVKWDESRFQEIVKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEATTNAPWYKGWEKETKAGVVKGKTLLEAIDAIEQPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPAGVTTEVKSVEMHHEQLEQGVPGDNVGFNVKNVSVKEIRRGNVCGDAKNDPPKGCASFNATVIVLNHPGQISAGYSPVLDCHTAHIACRFDELLEKNDRRSGKKLEDHPKFLKSGDAALVKFVPSKPMCVEAFSEYPPLGRFAVRDMRQTVAVGVIKSVDKTEKAAKVTKAAQKAAKK
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Keyword |
KW-0002--3D-structure KW-0009--Actin-binding KW-0181--Complete proteome KW-0963--Cytoplasm KW-0206--Cytoskeleton KW-0903--Direct protein sequencing KW-0251--Elongation factor KW-0342--GTP-binding KW-0488--Methylation KW-0547--Nucleotide-binding KW-0597--Phosphoprotein KW-0648--Protein biosynthesis KW-1185--Reference proteome
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Interpro |
IPR031157--G_TR_CS IPR027417--P-loop_NTPase IPR000795--TF_GTP-bd_dom IPR009000--Transl_B-barrel IPR009001--Transl_elong_EF1A/Init_IF2_C IPR004539--Transl_elong_EF1A_euk/arc IPR004161--Transl_elong_EFTu/EF1A_2 IPR004160--Transl_elong_EFTu/EF1A_C
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PROSITE |
PS00301--G_TR_1 PS51722--G_TR_2
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Pfam |
PF03144--GTP_EFTU_D2 PF03143--GTP_EFTU_D3
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Gene Ontology |
GO:0005737--C:cytoplasm GO:0005856--C:cytoskeleton GO:0005853--C:eukaryotic translation elongation factor 1 complex GO:0000329--C:fungal-type vacuole membrane GO:0005739--C:mitochondrion GO:0005840--C:ribosome GO:0019003--F:GDP binding GO:0005525--F:GTP binding GO:0003924--F:GTPase activity GO:0003746--F:translation elongation factor activity GO:0006414--P:translational elongation GO:0006409--P:tRNA export from nucleus
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