dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0002359
Uniprot Accession O70239; AXIN1_RAT;
Genbank Protein ID NP_077381.1;
Genbank Nucleotide ID NM_024405.1;
Protein Name Axin-1
Protein Synonyms/Alias Axis inhibition protein 1;rAxin;
Gene Name Axin1
Gene Synonyms/Alias Axin;
Organism Rattus norvegicus(Rat)
NCBI Taxa ID 10116
Functional Description
(View all)		 Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. Also facilitates the phosphorylation of APC by GSK3B. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity).
Phosphorylation Sites
 dbPAF PTMs: 14
PositionPeptidesSourceReferences ( PMIDs )
77YEPEGSASPTPPYLRcurated23984901
160NGIVSRQTKPATKSFPhosphoSitePlus22135298
164SRQTKPATKSFIKDCPhosphoSitePlus22135298
166QTKPATKSFIKDCVMPhosphoSitePlus22135298
275TQKLLLETAAPRAPSSysPTM 2.016641100; 19366988
282TAAPRAPSSRRYNEGdbPTM 3.0;PhosphoSitePlus;SysPTM 2.016641100; 16381945; 23193290; 22135298; 19366988
283AAPRAPSSRRYNEGRdbPTM 3.0;PhosphoSitePlus;SysPTM 2.016641100; 16381945; 23193290; 22135298; 19366988
317YALAPATSANDSEQQPhosphoSitePlus22135298
321PATSANDSEQQSLSSPhosphoSitePlus22135298
325ANDSEQQSLSSDADTPhosphoSitePlus22135298
480HVQRVMRTPGCQSPGPhosphoSitePlus22135298
485MRTPGCQSPGPGHRSPhosphoSitePlus22135298
492SPGPGHRSPDSGHVAPhosphoSitePlus22135298
787TKKGSYRYYFKKVSDPhosphoSitePlus22135298
Sequence
(Fasta)		MNVQEQGFPL DLGASFTEDA PRPPVPGEEG ELVSTDSRPV NHSFCSGKGT SIKSETSTAT 60
PRRSDLDLGY EPEGSASPTP PYLRWAESLH SLLDDQDGIS LFRTFLKQEG CADLLDFWFA 120
CSGFRKLEPC DSNEEKRLKL ARAIYRKYIL DSNGIVSRQT KPATKSFIKD CVMKQQIDPA 180
MFDQAQTEIQ STMEENTYPS FLKSDIYLEY TRTGSESPKV CSDQSSGSGT GKGMSGYLPT 240
LNEDEEWKCD QDADEDDGRD SVPPSRLTQK LLLETAAPRA PSSRRYNEGR ELRYGSWREP 300
VNPYYVNSGY ALAPATSAND SEQQSLSSDA DTLSLTDSSV DGIPPYRIRK QHRREMQESV 360
QVNGRVPLPH IPRTYRMPKE IRVEPQKFAE ELIHRLEAVQ RTREAEEKLE ERLKRVRMEE 420
EGEDGEMPSG PMASHKLPSV PAWHHFPPRY VDMGCSGLRD AHEENPESIL DEHVQRVMRT 480
PGCQSPGPGH RSPDSGHVAK TAVLGGTASG HGKHAPKLGL KLDSAGLHHH RHVHHHVHHN 540
SARPKEQMEA EAARRVQSSF SWGPETHGHA KPRSYSESTG TNPSAGDLAF GGKASAPSKR 600
NTKKAESGKN ASAEVPSTTE DAEKNQKIMQ WIIEGEKEIS RHRKAGHGSS GMRKQQAHES 660
SRPLSIERPG AVHPWVSAQL RNSVQPSHLF IQDPTMPPNP APNPLTQLEE ARRRLEEEEK 720
RANKLPSKQR TKSQRKAGGG SAPPCDSIVV AYYFCGEPIP YRTLVRGRAV TLGQFKELLT 780
KKGSYRYYFK KVSDEFDCGV VFEEVREDEA ILPVFEEKII GKVEKVD 828
Keyword

KW-0002--3D-structure
KW-0013--ADP-ribosylation
KW-0053--Apoptosis
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0217--Developmental protein
KW-1017--Isopeptide bond
KW-0472--Membrane
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0832--Ubl conjugation
KW-0879--Wnt signaling pathway
Interpro

IPR029797--AXIN1
IPR014936--Axin_b-cat-bd
IPR032101--Axin_TNKS_BD
IPR001158--DIX
IPR016137--RGS
IPR024066--RGS_subdom1
IPR029071--Ubiquitin-rel_dom
PROSITE

PS50841--DIX
PS50132--RGS
Pfam

PF16646--AXIN1_TNKS_BD
PF08833--Axin_b-cat_bind
PF00778--DIX
PF00615--RGS
Gene Ontology

GO:0030877--C:beta-catenin destruction complex
GO:0005938--C:cell cortex
GO:0005737--C:cytoplasm
GO:0016023--C:cytoplasmic membrane-bounded vesicle
GO:0005881--C:cytoplasmic microtubule
GO:0031410--C:cytoplasmic vesicle
GO:0005634--C:nucleus
GO:0005886--C:plasma membrane
GO:0014069--C:postsynaptic density
GO:0070016--F:armadillo repeat domain binding
GO:0008013--F:beta-catenin binding
GO:0005096--F:GTPase activator activity
GO:0042802--F:identical protein binding
GO:0019904--F:protein domain specific binding
GO:0042803--F:protein homodimerization activity
GO:0019901--F:protein kinase binding
GO:0005102--F:receptor binding
GO:0007257--P:activation of JUN kinase activity
GO:0006915--P:apoptotic process
GO:0048320--P:axial mesoderm formation
GO:0060070--P:canonical Wnt signaling pathway
GO:0060823--P:canonical Wnt signaling pathway involved in neural plate anterior/posterior pattern formation
GO:0008219--P:cell death
GO:0030154--P:cell differentiation
GO:0071407--P:cellular response to organic cyclic compound
GO:0031122--P:cytoplasmic microtubule organization
GO:0007368--P:determination of left/right symmetry
GO:0009950--P:dorsal/ventral axis specification
GO:0048048--P:embryonic eye morphogenesis
GO:0060272--P:embryonic skeletal joint morphogenesis
GO:0021797--P:forebrain anterior/posterior pattern specification
GO:0055001--P:muscle cell development
GO:0090090--P:negative regulation of canonical Wnt signaling pathway
GO:0045599--P:negative regulation of fat cell differentiation
GO:0034244--P:negative regulation of transcription elongation from RNA polymerase II promoter
GO:0030178--P:negative regulation of Wnt signaling pathway
GO:0030910--P:olfactory placode formation
GO:0001743--P:optic placode formation
GO:0043547--P:positive regulation of GTPase activity
GO:0046330--P:positive regulation of JNK cascade
GO:0045860--P:positive regulation of protein kinase activity
GO:2000060--P:positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0051260--P:protein homooligomerization
GO:0035412--P:regulation of catenin import into nucleus
GO:0016055--P:Wnt signaling pathway
GO:0090244--P:Wnt signaling pathway involved in somitogenesis
GO:0021881--P:Wnt-activated signaling pathway involved in forebrain neuron fate commitment