dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0002042
Uniprot Accession O60216; RAD21_HUMAN; A8K0E0; Q15001; Q99568;
Genbank Protein ID NP_006256.1;
Genbank Nucleotide ID NM_006265.2;
Protein Name Double-strand-break repair protein rad21 homolog
Protein Synonyms/Alias
Gene Name RAD21
Gene Synonyms/Alias HR21;KIAA0078;NXP1;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
Phosphorylation Sites
 dbPAF PTMs: 38 (View all)
PositionPeptidesSourceReferences ( PMIDs )
9FYAHFVLSKRGPLAKdbPTM 3.0;HPRD 9;PhosphoSitePlus19413330; 16381945; 23193290; 18988627; 22135298
40FECNLESSVESIISPPhosphoSitePlus22135298
46SSVESIISPKVKMALdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated18669648; 16381945; 23193290; 22067460; 15737063; 20068231;
55KVKMALRTSGHLLLGdbPTM 3.0;PHOSIDA;PhosphoSitePlus;curated19835603; 16381945; 23193290; 21081558; 22135298; 22817900
56VKMALRTSGHLLLGVdbPTM 3.0;PHOSIDA;PhosphoSitePlus;curated19835603; 16381945; 23193290; 21081558; 22135298; 22817900
67LLGVVRIYHRKAKYLdbPTM 3.0;PhosphoSitePlus;curated19835603; 16381945; 23193290; 22135298; 22817900
134IDVAQQFSLNQSRVEdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus22067460; 16381945; 23193290; 22135298
138QQFSLNQSRVEEITMdbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;curated22067460; 16381945; 23193290; 15737063; 22135298; 25627689;
144QSRVEEITMREEVGNdbPTM 3.0;PhosphoSitePlus;curated15737063; 16381945; 23193290; 22135298; 22817900
153REEVGNISILQENDFdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PHOSIDA;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated18669648; 19690332; 16381945; 23193290; 22067460; 15737063;
175REIMREGSAFEDDDMdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated18669648; 19690332; 16381945; 23193290; 22067460; 15737063;
185EDDDMLVSTTTSNLLPhosphoSitePlus;curated22135298; 25003641; 19690332
186DDDMLVSTTTSNLLLdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated18669648; 16381945; 23193290; 18988627; 22135298; 22817900
187DDMLVSTTTSNLLLEPhosphoSitePlus22135298
188DMLVSTTTSNLLLESPhosphoSitePlus22135298
189MLVSTTTSNLLLESEPhosphoSitePlus22135298
195TSNLLLESEQSTSNLcurated26074081
211EKINHLEYEDQYKDDdbPTM 3.0;PhosphoSitePlus;curated20562096; 16381945; 23193290; 22135298; 25627689; 22817900
215HLEYEDQYKDDNFGEdbPTM 3.0;PhosphoSitePlus;curated20562096; 16381945; 23193290; 22135298; 22817900
249FDDPPALSEAGVMLPPhosphoSitePlus;UniProt22135298; 24275569
Sequence
(Fasta)		MFYAHFVLSK RGPLAKIWLA AHWDKKLTKA HVFECNLESS VESIISPKVK MALRTSGHLL 60
LGVVRIYHRK AKYLLADCNE AFIKIKMAFR PGVVDLPEEN REAAYNAITL PEEFHDFDQP 120
LPDLDDIDVA QQFSLNQSRV EEITMREEVG NISILQENDF GDFGMDDREI MREGSAFEDD 180
DMLVSTTTSN LLLESEQSTS NLNEKINHLE YEDQYKDDNF GEGNDGGILD DKLISNNDGG 240
IFDDPPALSE AGVMLPEQPA HDDMDEDDNV SMGGPDSPDS VDPVEPMPTM TDQTTLVPNE 300
EEAFALEPID ITVKETKAKR KRKLIVDSVK ELDSKTIRAQ LSDYSDIVTT LDLAPPTKKL 360
MMWKETGGVE KLFSLPAQPL WNNRLLKLFT RCLTPLVPED LRKRRKGGEA DNLDEFLKEF 420
ENPEVPREDQ QQQHQQRDVI DEPIIEEPSR LQESVMEASR TNIDESAMPP PPPQGVKRKA 480
GQIDPEPVMP PQQVEQMEIP PVELPPEEPP NICQLIPELE LLPEKEKEKE KEKEDDEEEE 540
DEDASGGDQD QEERRWNKRT QQMLHGLQRA LAKTGAESIS LLELCRNTNR KQAAAKFYSF 600
LVLKKQQAIE LTQEEPYSDI IATPGPRFHI I 632
Keyword

KW-0002--3D-structure
KW-0053--Apoptosis
KW-0131--Cell cycle
KW-0132--Cell division
KW-0137--Centromere
KW-0158--Chromosome
KW-0159--Chromosome partition
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0225--Disease mutation
KW-0227--DNA damage
KW-0234--DNA repair
KW-0991--Mental retardation
KW-0498--Mitosis
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
Interpro

IPR023093--Rad21/Rec8_C
IPR006909--Rad21/Rec8_C_eu
IPR006910--Rad21_Rec8_N
IPR011991--WHTH_DNA-bd_dom
PROSITE
Pfam

PF04824--Rad21_Rec8
PF04825--Rad21_Rec8_N
Gene Ontology

GO:0000785--C:chromatin
GO:0005694--C:chromosome
GO:0000775--C:chromosome, centromeric region
GO:0008278--C:cohesin complex
GO:0005829--C:cytosol
GO:0016020--C:membrane
GO:0034991--C:nuclear meiotic cohesin complex
GO:0005654--C:nucleoplasm
GO:0001228--F:transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0006915--P:apoptotic process
GO:0051301--P:cell division
GO:0044267--P:cellular protein metabolic process
GO:0007059--P:chromosome segregation
GO:0006310--P:DNA recombination
GO:0006302--P:double-strand break repair
GO:0000278--P:mitotic cell cycle
GO:0007067--P:mitotic nuclear division
GO:0043687--P:post-translational protein modification
GO:0071168--P:protein localization to chromatin
GO:0016925--P:protein sumoylation
GO:0007131--P:reciprocal meiotic recombination
GO:0006357--P:regulation of transcription from RNA polymerase II promoter