dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0001888
Uniprot Accession O54952; BRCA1_RAT; P97951;
Genbank Protein ID NP_036646.1;
Genbank Nucleotide ID NM_012514.1;
Protein Name Breast cancer type 1 susceptibility protein homolog
Protein Synonyms/Alias
Gene Name Brca1
Gene Synonyms/Alias
Organism Rattus norvegicus(Rat)
NCBI Taxa ID 10116
Functional Description
(View all)
E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (By similarity).
Phosphorylation Sites
dbPAF PTMs: 7
PositionPeptidesSourceReferences ( PMIDs )
4----MDLSAVRIQEVcurated25532521
503NKLKRKRSTCLHPEDcurated23984901
686HIRKRSASDAFPEEKcurated23984901
706GLLTSCSSPRKPQGPcurated25532521; 23800682
717PQGPVNPSPERKGIEPhosphoSitePlus22135298
1298ENEEAFLSDKELISDcurated23984901
1561SQGQVAGSCRSPAAGcurated23984901
Sequence
(Fasta)
MDLSAVRIQE VQNVLHAMQK ILECPICLEL IKEPVSTQCD HIFCKFCMLK LLNQKKGPSQ 60
CPLCKNEITK RSLQGSARFS QLVEELLKII DAFELDTGMQ CANGFSFSKK KNSSSELLNE 120
DASIIQSVGY RNRVKKLQQI ESGSATLKDS LSVQLSNLGI VRSMKKNRQT QPQNKSVYIA 180
LESDSSEERV NAPDGCSVRD QELFQIAPGG AGDEGKLNSA KKAACDFSEG IRNIEHHQCS 240
DKDLNPTENH ATERHPEKCP RISVANVHVE PCGTDARASS LQRGTRSLLF TEDRLDAEKA 300
EFCDRSKQSG AAVSQQSRWA DSKETCNGRP VPRTEGKADP NVDSLCGRKQ WNHPKSLCPE 360
NSGATTDVPW ITLNSSIQKV NEWFSRTGEM LTSDNASDRR PASNAEAAVV LEVSNEVDGC 420
FSSSKKIDLV APDPDNAVMC TSGRDFSKPV ENIINDKIFG KTYQRKGSRP HLNHVTEIIG 480
TFTTEPQIIQ EQPFTNKLKR KRSTCLHPED FIKKADLTVV QRISENLNQG TDQMEPNDQA 540
MSITSNGQEN RATGNDLQRG RNAHPIESLR KEPAFTAKAK SISNSISDLE VELNVHSSKA 600
PKKNRLRRKS TRCVLPLEPI SRNPSPPTCA ELQIESCGSS EETKKNNSNQ TPAGHIREPQ 660
LIEDTEPAAD AKKNEPNEHI RKRSASDAFP EEKLMNKAGL LTSCSSPRKP QGPVNPSPER 720
KGIEQLEMCQ MPDNNKELGD LVLGGEPSGK PTEPSEESTS VSLVPDTDYD TQNSVSILEA 780
NTVRYARTGS VQCMTQFVAS ENPKELVHGS NNAGSGSECF KHPLRHELNH NQETIEMEDS 840
ELDTQYLQNT FQVSKRQSFA LFSKLRSPQK DCTLVGARSV PSREPSPKVT SRGEQKERQG 900
QEESEISHVQ AVTVTVGLPV PCQEGKPGAV TMCADVSRLC PSSHYRSCEN GLNTTDKSGI 960
SQNSHFRQSV SPLRSSIKTD NRKTLTEGRF EKHTERGMGN ETAVQSTIHT ISLNNRGDAC 1020
LEASSGSVIE VHSTGENVQG QLDRNRGPKV NTVSLLDSTQ PGVSKQSAPV SDKYLEIKQE 1080
SKAVSADFSP CLFSDHLEKP MRSDKTFQVC SETPDDLLDD VEIQENASFG EGGITEKSAI 1140
FNGSVLRRES SRSPSPVTHA SKSRSLHRGS RKLEFSEESD STEDEDLPCF QHLLSRVSST 1200
PELTRCSSVV TQRVPEKAKG TQAPRKSSIS DCNNEVILGE ASQEYQFSED AKCSGSMFSS 1260
QHSAALGSPA NALSQDPDFN PPSKQRRHQA ENEEAFLSDK ELISDHEDMA ACLEEASDQE 1320
EDSIIPDSVA SGYESEANLS EDCSQSDILT TQQRATMKDN LIKLQQEMAQ LEAVLEQHGS 1380
QPSGHPPCLP ADPCALEDLP DPEQNRSGTA ILTSKNINEN PVSQNPKRAC DDKSQPQPPD 1440
GLPSGDKESG MRRPSPFKSP LTSSRCSARG HSRSLQNRNS TSQEELLQPA XLEKSCEPHN 1500
LTGRSCLPRQ DLEGTPYPES GIRLVSSRDP DSESPKVSAL VCTAPASTSA LKISQGQVAG 1560
SCRSPAAGGA DTAVVEIVSK IKPEVTSPKE RAERDISMVV SGLTPKEVMI VQKFAEKYRL 1620
ALTDVITEET THVIIKTDAE FVCERTLKYF LGIAGGKWIV SYSWVIKSIQ ERKLLSVHEF 1680
EVKGDVVTGS NHQGPRRSRE SQEKLFEGLQ IYCCEPFTNM PKDELERMLQ LCGASVVKEL 1740
PLLTRDTGAH PIVLVQPSAW TEDNDCPDIG QLCKGRLVMW DWVLDSISVY RCRDLDAYLV 1800
QNITCGRDGS EPQDSND 1818
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0010--Activator
KW-0131--Cell cycle
KW-0158--Chromosome
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0227--DNA damage
KW-0233--DNA recombination
KW-0234--DNA repair
KW-0238--DNA-binding
KW-0275--Fatty acid biosynthesis
KW-0276--Fatty acid metabolism
KW-1017--Isopeptide bond
KW-0436--Ligase
KW-0444--Lipid biosynthesis
KW-0443--Lipid metabolism
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0043--Tumor suppressor
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR011364--BRCA1
IPR031099--BRCA1-associated
IPR025994--BRCA1_serine_dom
IPR001357--BRCT_dom
IPR018957--Znf_C3HC4_RING-type
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS

PROSITE

PS50172--BRCT
PS00518--ZF_RING_1
PS50089--ZF_RING_2

Pfam

PF00533--BRCT
PF12820--BRCT_assoc
PF00097--zf-C3HC4

Gene Ontology

GO:0070531--C:BRCA1-A complex
GO:0031436--C:BRCA1-BARD1 complex
GO:0005694--C:chromosome
GO:0005759--C:mitochondrial matrix
GO:0005634--C:nucleus
GO:0005886--C:plasma membrane
GO:0003682--F:chromatin binding
GO:0003677--F:DNA binding
GO:0016874--F:ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0008270--F:zinc ion binding
GO:0007420--P:brain development
GO:0007049--P:cell cycle
GO:0043009--P:chordate embryonic development
GO:0009048--P:dosage compensation by inactivation of X chromosome
GO:0000724--P:double-strand break repair via homologous recombination
GO:0006633--P:fatty acid biosynthetic process
GO:0045717--P:negative regulation of fatty acid biosynthetic process
GO:0035067--P:negative regulation of histone acetylation
GO:0071158--P:positive regulation of cell cycle arrest
GO:0035066--P:positive regulation of histone acetylation
GO:0033160--P:positive regulation of protein import into nucleus, translocation
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0045893--P:positive regulation of transcription, DNA-templated
GO:0051865--P:protein autoubiquitination
GO:0085020--P:protein K6-linked ubiquitination
GO:0042127--P:regulation of cell proliferation
GO:0032355--P:response to estradiol
GO:0033993--P:response to lipid
GO:0007584--P:response to nutrient
GO:0010033--P:response to organic substance
GO:0006351--P:transcription, DNA-templated