dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0001824
Uniprot Accession O54748; STK3_RAT;
Genbank Protein ID NP_113923.1;
Genbank Nucleotide ID NM_031735.1;
Protein Name Serine/threonine-protein kinase 3
Protein Synonyms/Alias Mammalian STE20-like protein kinase 2;MST-2;STE20-like kinase MST2;
Gene Name Stk3
Gene Synonyms/Alias Mst2;
Organism Rattus norvegicus(Rat)
NCBI Taxa ID 10116
Functional Description
(View all)
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38 (By similarity).
Phosphorylation Sites
dbPAF PTMs: 4
PositionPeptidesSourceReferences ( PMIDs )
15KSKLKKLSEDSLTKQcurated23712012; 21630457
180DTMAKRNTVIGTPFWdbPTM 3.012554736; 16381945; 23193290
316LEDEEENSDEDELDSPhosphoSitePlus;UniProt;curated22135298; 22673903; 25532521; 23712012; 21630457
384GTMKRNATSPQVQRPcurated23984901
Sequence
(Fasta)
MEQPPAPKSK LKKLSEDSLT KQPEEVFDVL EKLGEGSYGS VFKAIHKESG QVVAIKQVPV 60
ESDVQEIIKE ISIMQQCDSP YVVKYYGSYF KNTDLWIVME YCGAGSVSDI IRLRNKTLTE 120
DEIATILKST LKGLEYLHFM RKIHRDIKAG NILLNTEGHA KLADFGVAGQ LTDTMAKRNT 180
VIGTPFWMAP EVIQEIGYNC VADIWSLGIT SIEMAEGKPP YADIHPMRAI FMIPTNPPPT 240
FRKPELWSDD FTDFVKKCLV KSPEQRATAT QLLQHPFIKN AKPVSILREL ITEGMEIKAK 300
RHEEQQRELE DEEENSDEDE LDSHTMVKTS SEGVGTMRAT STMSEGAQTM IEHNSTMLES 360
DLGTMVINSE DEEEEDGTMK RNATSPQVQR PSFMDYFDKQ DFKNKSHENC DQSMREPCPM 420
SNNVFPDNWR VPQDGDFDFL KNLSLEELQM RLKALDPMME REIEELHQRY SAKRQPILDA 480
MDAKKRRQQN F 492
Keyword

KW-0007--Acetylation
KW-0053--Apoptosis
KW-0067--ATP-binding
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0418--Kinase
KW-0460--Magnesium
KW-0479--Metal-binding
KW-0547--Nucleotide-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0723--Serine/threonine-protein kinase
KW-0808--Transferase

Interpro

IPR011009--Kinase-like_dom
IPR024205--Mst1_SARAH_domain
IPR000719--Prot_kinase_dom
IPR017441--Protein_kinase_ATP_BS
IPR011524--SARAH_dom
IPR002290--Ser/Thr_dual-sp_kinase

PROSITE

PS00107--PROTEIN_KINASE_ATP
PS50011--PROTEIN_KINASE_DOM
PS50951--SARAH

Pfam

PF11629--Mst1_SARAH
PF00069--Pkinase

Gene Ontology

GO:0005737--C:cytoplasm
GO:0005634--C:nucleus
GO:0005524--F:ATP binding
GO:0004709--F:MAP kinase kinase kinase activity
GO:0046872--F:metal ion binding
GO:0004674--F:protein serine/threonine kinase activity
GO:0000186--P:activation of MAPKK activity
GO:0006915--P:apoptotic process
GO:0035329--P:hippo signaling
GO:0000165--P:MAPK cascade
GO:0006468--P:protein phosphorylation
GO:0042981--P:regulation of apoptotic process
GO:0007346--P:regulation of mitotic cell cycle
GO:0023014--P:signal transduction by protein phosphorylation
GO:0031098--P:stress-activated protein kinase signaling cascade