dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001687
Uniprot Accession O44081; DKC1_DROME; A8E745; Q0GLB4; Q0MVP1; Q8T408; Q9UAN8; Q9V3Z5;
Genbank Protein ID NP_001014547.1; NP_001163286.1; NP_001163289.1; NP_525120.1;
Genbank Nucleotide ID NM_001014547.4; NM_001169815.3; NM_001169818.3; NM_080381.5;
Protein Name H/ACA ribonucleoprotein complex subunit 4
Protein Synonyms/Alias Nucleolar protein AT band 60B;Protein minifly;
Gene Name Nop60B
Gene Synonyms/Alias mfl;CG3333;
Organism Drosophila melanogaster(Fruit fly)
NCBI Taxa ID 7227
Functional Description
(View all)		 Plays a central role in ribosomal RNA processing. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. Required for maintenance of the germline stem cell lineage during spermatogenesis.
Phosphorylation Sites
 dbPAF PTMs: 12
PositionPeptidesSourceReferences ( PMIDs )
75TPLAHGSSPLNRDIKPHOSIDA;PhosphoPep 2.0;curated21081558; 19060867; 22817900
428KPAAQEVSPTNGSSEPHOSIDA;PhosphoPep 2.0;curated21081558; 19060867; 22817900
430AAQEVSPTNGSSEPSPHOSIDA;PhosphoPep 2.0;curated21081558; 19060867; 22817900
433EVSPTNGSSEPSKRKPhosphoPep 2.019060867
434VSPTNGSSEPSKRKLPHOSIDA;curated21081558; 22817900
442EPSKRKLSTSSVEETdbPTM 3.0;Phospho.ELM 9.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18327897; 16381945; 23193290; 21081558; 19060867; 19366988;
443PSKRKLSTSSVEETAdbPTM 3.0;Phospho.ELM 9.0;PHOSIDA;PhosphoPep 2.0;UniProt;curated18327897; 16381945; 23193290; 21081558; 19060867; 22817900
444SKRKLSTSSVEETAAdbPTM 3.0;Phospho.ELM 9.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt;curated18327897; 16381945; 23193290; 21081558; 19060867; 19366988;
445KRKLSTSSVEETAAAdbPTM 3.0;Phospho.ELM 9.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt18327897; 16381945; 23193290; 21081558; 19060867; 19366988
449STSSVEETAAAAVSEdbPTM 3.0;Phospho.ELM 9.0;PHOSIDA;PhosphoPep 2.0;SysPTM 2.0;UniProt18327897; 16381945; 23193290; 21081558; 19060867; 19366988
455ETAAAAVSEETPSKDdbPTM 3.0;Phospho.ELM 9.0;UniProt18327897; 16381945; 23193290
458AAAVSEETPSKDKKKdbPTM 3.0;Phospho.ELM 9.0;SysPTM 2.0;UniProt18327897; 16381945; 23193290; 19366988
Sequence
(Fasta)		MADVEVRKEK KKKKIKEEPL DGDDIGTLQK QGNFQIKPSS KIAELDTSQW PLLLKNFDKL 60
NIRSNHYTPL AHGSSPLNRD IKEYMKTGFI NLDKPSNPSS HEVVAWIKKI LKVEKTGHSG 120
TLDPKVTGCL IVCIDRATRL VKSQQSAGKE YVAIFKLHGA VESVAKVRQG LEKLRGALFQ 180
RPPLISAVKR QLRVRTVYDS KLLDYDETRN MGVFWVSCEA GSYIRTMCVH LGLVLGVGGQ 240
MLELRRVRSG IQSERDGMVT MHDVLDAMWL YENHKDESML RRVIKPLEGL LVNHKRIIMK 300
DSSVNAVCYG AKITLPGVLR YEDGIEIDQE IVICTTKGEA ICLAIALMTT ATMASCDHGV 360
VAKIKRVIME RDTYPRKWGL GPKASAKKAL IAAGKLDKFG RPNENTPKEW LTGYVDYNAK 420
KPAAQEVSPT NGSSEPSKRK LSTSSVEETA AAAVSEETPS KDKKKKKKKH KGDEEAPEAA 480
EEEAEPVEKE KKKKKKKDKD RDRDEAQE 509
Keyword

KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0413--Isomerase
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0687--Ribonucleoprotein
KW-0690--Ribosome biogenesis
KW-0694--RNA-binding
KW-0698--rRNA processing
Interpro

IPR012960--Dyskerin-like
IPR002501--PsdUridine_synth
IPR020103--PsdUridine_synth_cat_dom
IPR002478--PUA
IPR015947--PUA-like_domain
IPR004802--tRNA_PsdUridine_synth_B_fam
PROSITE

PS50890--PUA
Pfam

PF08068--DKCLD
PF01472--PUA
PF01509--TruB_N
Gene Ontology

GO:0031429--C:box H/ACA snoRNP complex
GO:0005730--C:nucleolus
GO:0009982--F:pseudouridine synthase activity
GO:0003723--F:RNA binding
GO:0000495--P:box H/ACA snoRNA 3'-end processing
GO:0009267--P:cellular response to starvation
GO:0007281--P:germ cell development
GO:1990481--P:mRNA pseudouridine synthesis
GO:0022008--P:neurogenesis
GO:0001522--P:pseudouridine synthesis
GO:0042254--P:ribosome biogenesis
GO:0006364--P:rRNA processing
GO:0031118--P:rRNA pseudouridine synthesis
GO:0031120--P:snRNA pseudouridine synthesis
GO:0035220--P:wing disc development