dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001680
Uniprot Accession O43916; CHST1_HUMAN; D3DQP2;
Genbank Protein ID NP_003645.1;
Genbank Nucleotide ID NM_003654.5;
Protein Name Carbohydrate sulfotransferase 1
Protein Synonyms/Alias Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1;GST-1;Keratan sulfate Gal-6 sulfotransferase;KS6ST;KSGal6ST;KSST;
Gene Name CHST1
Gene Synonyms/Alias
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.
Phosphorylation Sites
 dbPAF PTMs: 7
PositionPeptidesSourceReferences ( PMIDs )
55EESPTFAYNLSRKTHdbPTM 3.0;PhosphoSitePlus21403953; 21447384; 16381945; 23193290; 22135298
120RRVMLGASRDLLRSLPhosphoSitePlus22135298
126ASRDLLRSLYDCDLYPhosphoSitePlus22135298
128RDLLRSLYDCDLYFLPhosphoSitePlus22135298
297PPWLKGKYMLVRYEDdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated20230923; 16381945; 23193290; 18988627; 22135298; 22817900
356TVRNSAATAEKWRFRPhosphoSitePlus22135298
398EEELKNPSVSLVEERdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated19664994; 16381945; 23193290; 18988627; 22135298; 22817900
Sequence
(Fasta)		MQCSWKAVLL LALASIAIQY TAIRTFTAKS FHTCPGLAEA GLAERLCEES PTFAYNLSRK 60
THILILATTR SGSSFVGQLF NQHLDVFYLF EPLYHVQNTL IPRFTQGKSP ADRRVMLGAS 120
RDLLRSLYDC DLYFLENYIK PPPVNHTTDR IFRRGASRVL CSRPVCDPPG PADLVLEEGD 180
CVRKCGLLNL TVAAEACRER SHVAIKTVRV PEVNDLRALV EDPRLNLKVI QLVRDPRGIL 240
ASRSETFRDT YRLWRLWYGT GRKPYNLDVT QLTTVCEDFS NSVSTGLMRP PWLKGKYMLV 300
RYEDLARNPM KKTEEIYGFL GIPLDSHVAR WIQNNTRGDP TLGKHKYGTV RNSAATAEKW 360
RFRLSYDIVA FAQNACQQVL AQLGYKIAAS EEELKNPSVS LVEERDFRPF S 412
Keyword

KW-0119--Carbohydrate metabolism
KW-0181--Complete proteome
KW-0325--Glycoprotein
KW-0333--Golgi apparatus
KW-0395--Inflammatory response
KW-0472--Membrane
KW-1185--Reference proteome
KW-0735--Signal-anchor
KW-0808--Transferase
KW-0812--Transmembrane
KW-1133--Transmembrane helix
Interpro

IPR016469--Carbohydrate_sulfotransferase
IPR027417--P-loop_NTPase
IPR000863--Sulfotransferase_dom
PROSITE
Pfam

PF00685--Sulfotransfer_1
Gene Ontology

GO:0000139--C:Golgi membrane
GO:0016021--C:integral component of membrane
GO:0045130--F:keratan sulfotransferase activity
GO:0001517--F:N-acetylglucosamine 6-O-sulfotransferase activity
GO:0008146--F:sulfotransferase activity
GO:0005975--P:carbohydrate metabolic process
GO:0006012--P:galactose metabolic process
GO:0030203--P:glycosaminoglycan metabolic process
GO:0006954--P:inflammatory response
GO:0018146--P:keratan sulfate biosynthetic process
GO:0042339--P:keratan sulfate metabolic process
GO:0005976--P:polysaccharide metabolic process
GO:0044281--P:small molecule metabolic process
GO:0006790--P:sulfur compound metabolic process