dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001648
Uniprot Accession O43809; CPSF5_HUMAN; Q6IB85; Q6NE84;
Genbank Protein ID NP_008937.1;
Genbank Nucleotide ID NM_007006.2;
Protein Name Cleavage and polyadenylation specificity factor subunit 5
Protein Synonyms/Alias Cleavage and polyadenylation specificity factor 25 kDa subunit;CFIm25;CPSF 25 kDa subunit;Nucleoside diphosphate-linked moiety X motif 21;Nudix motif 21;Pre-mRNA cleavage factor Im 25 kDa subunit;
Gene Name NUDT21
Gene Synonyms/Alias CFIM25;CPSF25;CPSF5;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.
Phosphorylation Sites
 dbPAF PTMs: 8
PositionPeptidesSourceReferences ( PMIDs )
9SVVPPNRSQTGWPRGPhosphoSitePlus;curated22135298; 23186163
24VTQFGNKYIQQTKPLPhosphoSitePlus22135298
36KPLTLERTINLYPLTPhosphoSitePlus22135298
40LERTINLYPLTNYTFdbPTM 3.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated15592455; 16381945; 23193290; 17360941; 18083107; 20090780;
45NLYPLTNYTFGTKEPPhosphoSitePlus22135298
49LTNYTFGTKEPLYEKPhosphoSitePlus22135298
101VLLLQLGTTFFKLPGdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 21081558; 22135298;
102LLLQLGTTFFKLPGGdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 21081558; 22135298;
Sequence
(Fasta)		MSVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV 60
AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG 120
LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ 180
EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN 228
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-0507--mRNA processing
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0694--RNA-binding
Interpro

IPR016706--Cleav_polyA_spec_factor_su5
IPR000086--NUDIX_hydrolase_dom
IPR015797--NUDIX_hydrolase_dom-like
PROSITE

PS51462--NUDIX
Pfam

PF13869--NUDIX_2
Gene Ontology

GO:0005813--C:centrosome
GO:0005815--C:microtubule organizing center
GO:0005849--C:mRNA cleavage factor complex
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0042382--C:paraspeckles
GO:0017091--F:AU-rich element binding
GO:0042826--F:histone deacetylase binding
GO:0016787--F:hydrolase activity
GO:0003729--F:mRNA binding
GO:0044822--F:poly(A) RNA binding
GO:0042803--F:protein homodimerization activity
GO:0010467--P:gene expression
GO:0031124--P:mRNA 3'-end processing
GO:0006378--P:mRNA polyadenylation
GO:0006397--P:mRNA processing
GO:0000398--P:mRNA splicing, via spliceosome
GO:0051262--P:protein tetramerization
GO:0008380--P:RNA splicing
GO:0006369--P:termination of RNA polymerase II transcription
GO:0006366--P:transcription from RNA polymerase II promoter