dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001644
Uniprot Accession O43791; SPOP_HUMAN; B2R6S3; D3DTW7; Q53HJ1;
Genbank Protein ID NP_001007227.1; NP_001007228.1; NP_001007229.1; NP_001007230.1; NP_001007231.1; NP_003554.1; XP_005257780.1; XP_005257781.1;
Genbank Nucleotide ID NM_001007226.1; NM_001007227.1; NM_001007228.1; NM_001007229.1; NM_001007230.1; NM_003563.3; XM_005257723.3; XM_005257724.3;
Protein Name Speckle-type POZ protein
Protein Synonyms/Alias HIB homolog 1;Roadkill homolog 1;
Gene Name SPOP
Gene Synonyms/Alias
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.
Phosphorylation Sites
 dbPAF PTMs: 3
PositionPeptidesSourceReferences ( PMIDs )
6--MSRVPSPPPPAEMPhosphoSitePlus;curated22135298; 25627689; 26074081
54EMGEVIKSSTFSSGAPhosphoSitePlus22135298
353PHLVAEAYRSLASAQdbPTM 3.0;PhosphoSitePlus;curated18083107; 16381945; 23193290; 22135298; 22817900
Sequence
(Fasta)		MSRVPSPPPP AEMSSGPVAE SWCYTQIKVV KFSYMWTINN FSFCREEMGE VIKSSTFSSG 60
ANDKLKWCLR VNPKGLDEES KDYLSLYLLL VSCPKSEVRA KFKFSILNAK GEETKAMESQ 120
RAYRFVQGKD WGFKKFIRRD FLLDEANGLL PDDKLTLFCE VSVVQDSVNI SGQNTMNMVK 180
VPECRLADEL GGLWENSRFT DCCLCVAGQE FQAHKAILAA RSPVFSAMFE HEMEESKKNR 240
VEINDVEPEV FKEMMCFIYT GKAPNLDKMA DDLLAAADKY ALERLKVMCE DALCSNLSVE 300
NAAEILILAD LHSADQLKTQ AVDFINYHAS DVLETSGWKS MVVSHPHLVA EAYRSLASAQ 360
CPFLGPPRKR LKQS 375
Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway
Interpro

IPR000210--BTB/POZ_dom
IPR002083--MATH/TRAF_dom
IPR011333--POZ_dom
IPR008974--TRAF-like
PROSITE

PS50097--BTB
PS50144--MATH
Pfam

PF00651--BTB
PF00917--MATH
Gene Ontology

GO:0031463--C:Cul3-RING ubiquitin ligase complex
GO:0016607--C:nuclear speck
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0031625--F:ubiquitin protein ligase binding
GO:0042593--P:glucose homeostasis
GO:0043433--P:negative regulation of sequence-specific DNA binding transcription factor activity
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:1902237--P:positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
GO:2000676--P:positive regulation of type B pancreatic cell apoptotic process
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0042787--P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process