dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001612
Uniprot Accession O43684; BUB3_HUMAN; A6NJ42; B2R6E7; D3DRE9; O43685;
Genbank Protein ID NP_001007794.1; NP_004716.1;
Genbank Nucleotide ID NM_001007793.2; NM_004725.3;
Protein Name Mitotic checkpoint protein BUB3
Protein Synonyms/Alias
Gene Name BUB3
Gene Synonyms/Alias
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Phosphorylation Sites
 dbPAF PTMs: 11
PositionPeptidesSourceReferences ( PMIDs )
19PPEDGISSVKFSPNTdbPTM 3.0;PhosphoSitePlus;curated20057499; 16381945; 23193290; 22135298; 22817900
33TSQFLLVSSWDTSVRPhosphoSitePlus22135298
127VKLWDPRTPCNAGTFPhosphoSitePlus;curated22135298; 25627689
141FSQPEKVYTLSVSGDPhosphoSitePlus22135298
144PEKVYTLSVSGDRLIPhosphoSitePlus22135298
154GDRLIVGTAGRRVLVdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated20068230; 16381945; 23193290; 18988627; 22135298; 22817900;
194AFPNKQGYVLSSIEGdbPTM 3.0;HPRD 9;PhosphoSitePlus;SysPTM 2.017322306; 16381945; 23193290; 18988627; 22135298; 19366988
207EGRVAVEYLDPSPEVPhosphoSitePlus;curated22135298; 22817900
211AVEYLDPSPEVQKKKdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated15302935; 18669648; 16381945; 23193290; 22067460; 19664994;
325DAETKPKSPCT----dbPTM 3.0;Phospho.ELM 9.0;PhosphoSitePlus;curated22067460; 16381945; 23193290; 22135298; 23898821; 25849741
328TKPKSPCT-------dbPTM 3.0;PhosphoSitePlus22067460; 16381945; 23193290; 22135298
Sequence
(Fasta)		MTGSNEFKLN QPPEDGISSV KFSPNTSQFL LVSSWDTSVR LYDVPANSMR LKYQHTGAVL 60
DCAFYDPTHA WSGGLDHQLK MHDLNTDQEN LVGTHDAPIR CVEYCPEVNV MVTGSWDQTV 120
KLWDPRTPCN AGTFSQPEKV YTLSVSGDRL IVGTAGRRVL VWDLRNMGYV QQRRESSLKY 180
QTRCIRAFPN KQGYVLSSIE GRVAVEYLDP SPEVQKKKYA FKCHRLKENN IEQIYPVNAI 240
SFHNIHNTFA TGGSDGFVNI WDPFNKKRLC QFHRYPTSIA SLAFSNDGTT LAIASSYMYE 300
MDDTEHPEDG IFIRQVTDAE TKPKSPCT 329
Keyword

KW-0007--Acetylation
KW-0013--ADP-ribosylation
KW-0025--Alternative splicing
KW-0131--Cell cycle
KW-0132--Cell division
KW-0137--Centromere
KW-0158--Chromosome
KW-0159--Chromosome partition
KW-0181--Complete proteome
KW-1017--Isopeptide bond
KW-0995--Kinetochore
KW-0469--Meiosis
KW-0498--Mitosis
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0832--Ubl conjugation
KW-0853--WD repeat
Interpro

IPR020472--G-protein_beta_WD-40_rep
IPR015943--WD40/YVTN_repeat-like_dom
IPR001680--WD40_repeat
IPR017986--WD40_repeat_dom
PROSITE

PS50082--WD_REPEATS_2
PS50294--WD_REPEATS_REGION
Pfam

PF00400--WD40
Gene Ontology

GO:0000777--C:condensed chromosome kinetochore
GO:0005829--C:cytosol
GO:0000776--C:kinetochore
GO:0005654--C:nucleoplasm
GO:0031145--P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0008608--P:attachment of spindle microtubules to kinetochore
GO:0051301--P:cell division
GO:0051321--P:meiotic cell cycle
GO:0000278--P:mitotic cell cycle
GO:0000070--P:mitotic sister chromatid segregation
GO:0007094--P:mitotic spindle assembly checkpoint
GO:0051436--P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0051439--P:regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0007264--P:small GTPase mediated signal transduction
GO:0071173--P:spindle assembly checkpoint