dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001599
Uniprot Accession O43639; NCK2_HUMAN; D3DVK1; Q9BWN9; Q9UIC3;
Genbank Protein ID NP_001004720.1; NP_003572.2; XP_006712860.1; XP_011510293.1;
Genbank Nucleotide ID NM_001004720.2; NM_003581.4; XM_006712797.2; XM_011511991.1;
Protein Name Cytoplasmic protein NCK2
Protein Synonyms/Alias Growth factor receptor-bound protein 4;NCK adaptor protein 2;Nck-2;SH2/SH3 adaptor protein NCK-beta;
Gene Name NCK2
Gene Synonyms/Alias GRB4;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
Phosphorylation Sites
 dbPAF PTMs: 20
PositionPeptidesSourceReferences ( PMIDs )
37WLLDDSKTWWRVRNAPhosphoSitePlus22135298
48VRNAANRTGYVPSNYPhosphoSitePlus22135298
50NAANRTGYVPSNYVEdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated18083107; 20090780; 16381945; 23193290; 19060867; 22135298;
53NRTGYVPSNYVERKNcurated23882029
61NYVERKNSLKKGSLVPhosphoSitePlus;curated22135298; 23882029
66KNSLKKGSLVKNLKDPhosphoSitePlus22135298
74LVKNLKDTLGLGKTRPhosphoSitePlus;curated22135298; 25003641
84LGKTRRKTSARDASPcurated23090842
85GKTRRKTSARDASPTcurated23090842
90KTSARDASPTPSTDAdbPTM 3.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;UniProt;curated19690332; 16381945; 23193290; 19664994; 19664995; 20007894;
92SARDASPTPSTDAEYdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;curated18088087; 16381945; 23193290; 19690332; 15345747; 18988627;
94RDASPTPSTDAEYPAdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21406692; 22115753; 16381945; 23193290; 19060867; 22135298;
95DASPTPSTDAEYPANPhosphoSitePlus;curated22135298; 19690332
99TPSTDAEYPANGSGAdbPTM 3.0;PhosphoSitePlus;curated18083107; 20090780; 16381945; 23193290; 22135298; 22817900;
104AEYPANGSGADRIYDPhosphoSitePlus;curated22135298; 26267517
110GSGADRIYDLNIPAFdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoPep 2.0;PhosphoSitePlus;SysPTM 2.0;UniProt;curated18088087; 16381945; 23193290; 15570572; 18083107; 20007894;
176VDEAAAESPSFLSLRcurated26074081
271AHAPQISYTGPSSSGPhosphoSitePlus22135298
321ESSPSDFSVSLKASGPhosphoSitePlus;curated22135298; 23186163
342VQLVDNVYCIGQRRFdbPTM 3.0;PhosphoPep 2.0;PhosphoSitePlus;curated21253578; 16381945; 23193290; 19060867; 22135298; 24400094;
Sequence
(Fasta)		MTEEVIVIAK WDYTAQQDQE LDIKKNERLW LLDDSKTWWR VRNAANRTGY VPSNYVERKN 60
SLKKGSLVKN LKDTLGLGKT RRKTSARDAS PTPSTDAEYP ANGSGADRIY DLNIPAFVKF 120
AYVAEREDEL SLVKGSRVTV MEKCSDGWWR GSYNGQIGWF PSNYVLEEVD EAAAESPSFL 180
SLRKGASLSN GQGSRVLHVV QTLYPFSSVT EEELNFEKGE TMEVIEKPEN DPEWWKCKNA 240
RGQVGLVPKN YVVVLSDGPA LHPAHAPQIS YTGPSSSGRF AGREWYYGNV TRHQAECALN 300
ERGVEGDFLI RDSESSPSDF SVSLKASGKN KHFKVQLVDN VYCIGQRRFH TMDELVEHYK 360
KAPIFTSEHG EKLYLVRALQ 381
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0256--Endoplasmic reticulum
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0727--SH2 domain
KW-0728--SH3 domain
KW-0810--Translation regulation
Interpro

IPR017304--NCK
IPR000980--SH2
IPR001452--SH3_domain
PROSITE

PS50001--SH2
PS50002--SH3
Pfam

PF00017--SH2
PF00018--SH3_1
PF14604--SH3_9
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005783--C:endoplasmic reticulum
GO:0097481--C:neuronal postsynaptic density
GO:0012506--C:vesicle membrane
GO:0008093--F:cytoskeletal adaptor activity
GO:0030159--F:receptor signaling complex scaffold activity
GO:0005070--F:SH3/SH2 adaptor activity
GO:0007015--P:actin filament organization
GO:0007411--P:axon guidance
GO:0016477--P:cell migration
GO:0060996--P:dendritic spine development
GO:0048013--P:ephrin receptor signaling pathway
GO:0007173--P:epidermal growth factor receptor signaling pathway
GO:0001771--P:immunological synapse formation
GO:0030032--P:lamellipodium assembly
GO:0008285--P:negative regulation of cell proliferation
GO:1903912--P:negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation
GO:0033137--P:negative regulation of peptidyl-serine phosphorylation
GO:1903898--P:negative regulation of PERK-mediated unfolded protein response
GO:1990441--P:negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
GO:0030838--P:positive regulation of actin filament polymerization
GO:1902237--P:positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
GO:0042102--P:positive regulation of T cell proliferation
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0036493--P:positive regulation of translation in response to endoplasmic reticulum stress
GO:0007176--P:regulation of epidermal growth factor-activated receptor activity
GO:0007172--P:signal complex assembly
GO:0007165--P:signal transduction
GO:0042110--P:T cell activation
GO:0048010--P:vascular endothelial growth factor receptor signaling pathway