dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001578
Uniprot Accession O43566; RGS14_HUMAN; O43565; Q506M1; Q6ZWA4; Q8TD62;
Genbank Protein ID NP_006471.2;
Genbank Nucleotide ID NM_006480.4;
Protein Name Regulator of G-protein signaling 14
Protein Synonyms/Alias
Gene Name RGS14
Gene Synonyms/Alias
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not G(o) alpha subunit GNAO1 and G(i) alpha subunit GNAI2. Confers GAP activity on G(o) alpha subunit GNAI0 and G(i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division. Probably required for the nerve growth factor (NGF)-mediated neurite outgrowth. May be involved in visual memory processing capacity and hippocampal-based learning and memory.
Phosphorylation Sites
 dbPAF PTMs: 31 (View all)
PositionPeptidesSourceReferences ( PMIDs )
20GRMVLAVSDGELSSTdbPTM 3.0;PhosphoSitePlus;UniProt;curated19690332; 16381945; 23193290; 22135298; 26074081
25AVSDGELSSTTGPQGcurated19690332
39GQGEGRGSSLSIHSLcurated22115753; 19690332
40QGEGRGSSLSIHSLPdbPTM 3.0;PhosphoSitePlus;curated19690332; 16381945; 23193290; 22135298
42EGRGSSLSIHSLPSGdbPTM 3.0;PhosphoSitePlus;UniProt;curated19690332; 16381945; 23193290; 22135298
45GSSLSIHSLPSGPSSdbPTM 3.0;PhosphoSitePlus;UniProt;curated19690332; 16381945; 23193290; 22115753; 22135298
48LSIHSLPSGPSSPFPPhosphoSitePlus;curated22135298; 19690332
52SLPSGPSSPFPTEEQPhosphoSitePlus22135298
67PVASWALSFERLLQDdbPTM 3.0;HPRD 9;PhosphoSitePlus20058876; 16381945; 23193290; 18988627; 22135298
90EFLKKEFSAENVTFWcurated26074081
122AQEARNIYQEFLSSQdbPTM 3.0;PhosphoSitePlus;curated19690332; 20090780; 16381945; 23193290; 22135298
199PLREPGSSRLGSPDAPhosphoSitePlus;curated22135298; 23186163
203PGSSRLGSPDATRKKPhosphoSitePlus;curated22135298; 24702127
218PKLKPGKSLPLGVEEdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated19690332; 20058876; 22115753; 16381945; 23193290; 18988627;
243GGRPLRKSFRRELGGPhosphoSitePlus;curated22135298; 26074081
251FRRELGGTANAALRRPhosphoSitePlus22135298
260NAALRRESQGSLNSSdbPTM 3.0;Phospho.ELM 9.0;HPRD 9;PhosphoSitePlus;curated19690332; 16381945; 23193290; 12534294; 18988627; 22135298
263LRRESQGSLNSSASLPhosphoSitePlus;curated22135298; 19690332
269GSLNSSASLDLGFLAcurated19690332
288KSESHRKSLGSTEGEdbPTM 3.0;PhosphoSitePlus;curated20164059; 22115753; 16381945; 23193290; 22135298; 25850435;
Sequence
(Fasta)		MPGKPKHLGV PNGRMVLAVS DGELSSTTGP QGQGEGRGSS LSIHSLPSGP SSPFPTEEQP 60
VASWALSFER LLQDPLGLAY FTEFLKKEFS AENVTFWKAC ERFQQIPASD TQQLAQEARN 120
IYQEFLSSQA LSPVNIDRQA WLGEEVLAEP RPDMFRAQQL QIFNLMKFDS YARFVKSPLY 180
RECLLAEAEG RPLREPGSSR LGSPDATRKK PKLKPGKSLP LGVEELGQLP PVEGPGGRPL 240
RKSFRRELGG TANAALRRES QGSLNSSASL DLGFLAFVSS KSESHRKSLG STEGESESRP 300
GKYCCVYLPD GTASLALARP GLTIRDMLAG ICEKRGLSLP DIKVYLVGNE QALVLDQDCT 360
VLADQEVRLE NRITFELELT ALERVVRISA KPTKRLQEAL QPILEKHGLS PLEVVLHRPG 420
EKQPLDLGKL VSSVAAQRLV LDTLPGVKIS KARDKSPCRS QGCPPRTQDK ATHPPPASPS 480
SLVKVPSSAT GKRQTCDIEG LVELLNRVQS SGAHDQRGLL RKEDLVLPEF LQLPAQGPSS 540
EETPPQTKSA AQPIGGSLNS TTDSAL 567
Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0131--Cell cycle
KW-0132--Cell division
KW-0965--Cell junction
KW-1003--Cell membrane
KW-0966--Cell projection
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0343--GTPase activation
KW-0472--Membrane
KW-0493--Microtubule
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0628--Postsynaptic cell membrane
KW-1185--Reference proteome
KW-0677--Repeat
KW-0734--Signal transduction inhibitor
KW-0770--Synapse
Interpro

IPR003109--GoLoco_motif
IPR003116--Raf-like_ras-bd
IPR016137--RGS
IPR030776--RGS14
IPR024066--RGS_subdom1
IPR029071--Ubiquitin-rel_dom
PROSITE

PS50877--GOLOCO
PS50898--RBD
PS50132--RGS
Pfam

PF02196--RBD
PF00615--RGS
Gene Ontology

GO:0030054--C:cell junction
GO:0005813--C:centrosome
GO:0005737--C:cytoplasm
GO:0030425--C:dendrite
GO:0043197--C:dendritic spine
GO:0005874--C:microtubule
GO:0016604--C:nuclear body
GO:0005634--C:nucleus
GO:0005886--C:plasma membrane
GO:0016605--C:PML body
GO:0014069--C:postsynaptic density
GO:0045211--C:postsynaptic membrane
GO:0005819--C:spindle
GO:0000922--C:spindle pole
GO:0005092--F:GDP-dissociation inhibitor activity
GO:0005096--F:GTPase activator activity
GO:0008017--F:microtubule binding
GO:0030159--F:receptor signaling complex scaffold activity
GO:0005057--F:receptor signaling protein activity
GO:0051301--P:cell division
GO:0007059--P:chromosome segregation
GO:0035556--P:intracellular signal transduction
GO:0007612--P:learning
GO:0007616--P:long-term memory
GO:0060291--P:long-term synaptic potentiation
GO:0007067--P:mitotic nuclear division
GO:0070373--P:negative regulation of ERK1 and ERK2 cascade
GO:0043407--P:negative regulation of MAP kinase activity
GO:0031914--P:negative regulation of synaptic plasticity
GO:0006913--P:nucleocytoplasmic transport
GO:0048008--P:platelet-derived growth factor receptor signaling pathway
GO:0043547--P:positive regulation of GTPase activity
GO:0050769--P:positive regulation of neurogenesis
GO:0043620--P:regulation of DNA-templated transcription in response to stress
GO:0008277--P:regulation of G-protein coupled receptor protein signaling pathway
GO:0006979--P:response to oxidative stress
GO:0007051--P:spindle organization
GO:0008542--P:visual learning
GO:0010070--P:zygote asymmetric cell division