dbPAF Protein Information


Tag Content
dbPAF ID dbPAF-0001548
Uniprot Accession O43474; KLF4_HUMAN; B2R8S4; B3KT79; L0R3I6; L0R4N5; P78338; Q5T3J8; Q5T3J9; Q8N717; Q9UNP3;
Genbank Protein ID NP_004226.3;
Genbank Nucleotide ID NM_004235.5;
Protein Name Krueppel-like factor 4
Protein Synonyms/Alias Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor;
Gene Name KLF4
Gene Synonyms/Alias EZF;GKLF;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Phosphorylation Sites
dbPAF PTMs: 15
PositionPeptidesSourceReferences ( PMIDs )
234GKFVLKASLSAPGSEPhosphoSitePlus;curated22135298; 25627689; 23186163
236FVLKASLSAPGSEYGdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 22135298; 25627689
240ASLSAPGSEYGSPSVdbPTM 3.018220336; 16381945; 23193290
244APGSEYGSPSVISVSdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 21081558; 22135298;
246GSEYGSPSVISVSKGcurated25627689
249YGSPSVISVSKGSPDPhospho.ELM 9.018220336
254VISVSKGSPDGSHPVdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;UniProt;curated18220336; 18669648; 19276368; 20068231; 16381945; 23193290;
258SKGSPDGSHPVVVAPdbPTM 3.0;PhosphoSitePlus;curated18220336; 16381945; 23193290; 22135298; 25627689; 21857030
316RQLPSRTTPTLGLEEdbPTM 3.0;HPRD 9;PhosphoSitePlus;curated18669648; 16381945; 23193290; 18988627; 22135298; 23186163;
327GLEEVLSSRDCHPALPhosphoSitePlus22135298
444CGKTYTKSSHLKAHLHPRD 9;PhosphoSitePlus20166139; 18988627; 22135298
445GKTYTKSSHLKAHLRHPRD 9;PhosphoSitePlus20166139; 18988627; 22135298
453HLKAHLRTHTGEKPYPhosphoSitePlus22135298
455KAHLRTHTGEKPYHCPhosphoSitePlus22135298
485TRHYRKHTGHRPFQCPhosphoSitePlus22135298
Sequence
(Fasta)
MRQPPGESDM AVSDALLPSF STFASGPAGR EKTLRQAGAP NNRWREELSH MKRLPPVLPG 60
RPYDLAAATV ATDLESGGAG AACGGSNLAP LPRRETEEFN DLLDLDFILS NSLTHPPESV 120
AATVSSSASA SSSSSPSSSG PASAPSTCSF TYPIRAGNDP GVAPGGTGGG LLYGRESAPP 180
PTAPFNLADI NDVSPSGGFV AELLRPELDP VYIPPQQPQP PGGGLMGKFV LKASLSAPGS 240
EYGSPSVISV SKGSPDGSHP VVVAPYNGGP PRTCPKIKQE AVSSCTHLGA GPPLSNGHRP 300
AAHDFPLGRQ LPSRTTPTLG LEEVLSSRDC HPALPLPPGF HPHPGPNYPS FLPDQMQPQV 360
PPLHYQGQSR GFVARAGEPC VCWPHFGTHG MMLTPPSSPL ELMPPGSCMP EEPKPKRGRR 420
SWPRKRTATH TCDYAGCGKT YTKSSHLKAH LRTHTGEKPY HCDWDGCGWK FARSDELTRH 480
YRKHTGHRPF QCQKCDRAFS RSDHLALHMK RHF 514
Keyword

KW-0010--Activator
KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-1017--Isopeptide bond
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0677--Repeat
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0832--Ubl conjugation
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR007087--Znf_C2H2
IPR015880--Znf_C2H2-like
IPR013087--Znf_C2H2/integrase_DNA-bd

PROSITE

PS00028--ZINC_FINGER_C2H2_1
PS50157--ZINC_FINGER_C2H2_2

Pfam

PF00096--zf-C2H2

Gene Ontology

GO:0000790--C:nuclear chromatin
GO:0005719--C:nuclear euchromatin
GO:0044798--C:nuclear transcription factor complex
GO:0005654--C:nucleoplasm
GO:0001047--F:core promoter binding
GO:0000987--F:core promoter proximal region sequence-specific DNA binding
GO:0035014--F:phosphatidylinositol 3-kinase regulator activity
GO:0001085--F:RNA polymerase II transcription factor binding
GO:0003700--F:transcription factor activity, sequence-specific DNA binding
GO:0001010--F:transcription factor activity, sequence-specific DNA binding transcription factor recruiting
GO:0044212--F:transcription regulatory region DNA binding
GO:0001077--F:transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0001190--F:transcriptional activator activity, RNA polymerase II transcription factor binding
GO:0008270--F:zinc ion binding
GO:0060070--P:canonical Wnt signaling pathway
GO:0071409--P:cellular response to cycloheximide
GO:0071363--P:cellular response to growth factor stimulus
GO:0070301--P:cellular response to hydrogen peroxide
GO:0071499--P:cellular response to laminar fluid shear stress
GO:1901653--P:cellular response to peptide
GO:0071300--P:cellular response to retinoic acid
GO:0009913--P:epidermal cell differentiation
GO:0048730--P:epidermis morphogenesis
GO:0045444--P:fat cell differentiation
GO:0007500--P:mesodermal cell fate determination
GO:0090051--P:negative regulation of cell migration involved in sprouting angiogenesis
GO:0008285--P:negative regulation of cell proliferation
GO:2000342--P:negative regulation of chemokine (C-X-C motif) ligand 2 production
GO:0043154--P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0070373--P:negative regulation of ERK1 and ERK2 cascade
GO:0034115--P:negative regulation of heterotypic cell-cell adhesion
GO:0050728--P:negative regulation of inflammatory response
GO:0045415--P:negative regulation of interleukin-8 biosynthetic process
GO:0014740--P:negative regulation of muscle hyperplasia
GO:0032088--P:negative regulation of NF-kappaB transcription factor activity
GO:0014067--P:negative regulation of phosphatidylinositol 3-kinase signaling
GO:0051898--P:negative regulation of protein kinase B signaling
GO:0060761--P:negative regulation of response to cytokine stimulus
GO:0048662--P:negative regulation of smooth muscle cell proliferation
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0032270--P:positive regulation of cellular protein metabolic process
GO:0046985--P:positive regulation of hemoglobin biosynthetic process
GO:0045429--P:positive regulation of nitric oxide biosynthetic process
GO:0051247--P:positive regulation of protein metabolic process
GO:0051973--P:positive regulation of telomerase activity
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0045893--P:positive regulation of transcription, DNA-templated
GO:0031077--P:post-embryonic camera-type eye development
GO:0035166--P:post-embryonic hemopoiesis
GO:0045595--P:regulation of cell differentiation
GO:0035019--P:somatic stem cell population maintenance
GO:0019827--P:stem cell population maintenance
GO:0006366--P:transcription from RNA polymerase II promoter