dbPAF Protein Information

Tag Content
dbPAF ID dbPAF-0001484
Uniprot Accession O43252; PAPS1_HUMAN; O43841; O75332; Q96FB1; Q96TF4; Q9P1P9; Q9UE98;
Genbank Protein ID NP_005434.4; XP_011530702.1; XP_011530703.1;
Genbank Nucleotide ID NM_005443.4; XM_011532400.1; XM_011532401.1;
Protein Name Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Protein Synonyms/Alias
Gene Name PAPSS1
Gene Synonyms/Alias ATPSK1;PAPSS;
Organism Homo sapiens(Human)
NCBI Taxa ID 9606
Functional Description
(View all)		 Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.
Phosphorylation Sites
 dbPAF PTMs: 8
PositionPeptidesSourceReferences ( PMIDs )
6--MEIPGSLCKKVKLPhosphoSitePlus;curated22135298; 25627689
14LCKKVKLSNNAQNWGdbPTM 3.0;PhosphoSitePlus22067460; 16381945; 23193290; 22135298
29MQRATNVTYQAHHVSPhosphoSitePlus22135298
30QRATNVTYQAHHVSRdbPTM 3.0;PhosphoSitePlus;curated17360941; 21394647; 16381945; 23193290; 22135298; 22817900
36TYQAHHVSRNKRGQVPhosphoSitePlus22135298
102LNKNLGFSPEDREENcurated26074081
518MVAGANFYIVGRDPAdbPTM 3.0;HPRD 9;PHOSIDA;PhosphoSitePlus;curated20068231; 16381945; 23193290; 18988627; 21081558; 22135298;
537PETGKDLYEPSHGAKPhosphoSitePlus22135298
Sequence
(Fasta)		MEIPGSLCKK VKLSNNAQNW GMQRATNVTY QAHHVSRNKR GQVVGTRGGF RGCTVWLTGL 60
SGAGKTTVSM ALEEYLVCHG IPCYTLDGDN IRQGLNKNLG FSPEDREENV RRIAEVAKLF 120
ADAGLVCITS FISPYTQDRN NARQIHEGAS LPFFEVFVDA PLHVCEQRDV KGLYKKARAG 180
EIKGFTGIDS EYEKPEAPEL VLKTDSCDVN DCVQQVVELL QERDIVPVDA SYEVKELYVP 240
ENKLHLAKTD AETLPALKIN KVDMQWVQVL AEGWATPLNG FMREREYLQC LHFDCLLDGG 300
VINLSVPIVL TATHEDKERL DGCTAFALMY EGRRVAILRN PEFFEHRKEE RCARQWGTTC 360
KNHPYIKMVM EQGDWLIGGD LQVLDRVYWN DGLDQYRLTP TELKQKFKDM NADAVFAFQL 420
RNPVHNGHAL LMQDTHKQLL ERGYRRPVLL LHPLGGWTKD DDVPLMWRMK QHAAVLEEGV 480
LNPETTVVAI FPSPMMYAGP TEVQWHCRAR MVAGANFYIV GRDPAGMPHP ETGKDLYEPS 540
HGAKVLTMAP GLITLEIVPF RVAAYNKKKK RMDYYDSEHH EDFEFISGTR MRKLAREGQK 600
PPEGFMAPKA WTVLTEYYKS LEKA 625
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0067--ATP-binding
KW-0181--Complete proteome
KW-0418--Kinase
KW-0511--Multifunctional enzyme
KW-0547--Nucleotide-binding
KW-0548--Nucleotidyltransferase
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0808--Transferase
Interpro

IPR002891--APS_kinase
IPR025980--ATP-Sase_PUA-like_dom
IPR027417--P-loop_NTPase
IPR015947--PUA-like_domain
IPR014729--Rossmann-like_a/b/a_fold
IPR024951--Sulfurylase_cat_dom
IPR002650--Sulphate_adenylyltransferase
PROSITE
Pfam

PF01583--APS_kinase
PF01747--ATP-sulfurylase
PF14306--PUA_2
Gene Ontology

GO:0005829--C:cytosol
GO:0004020--F:adenylylsulfate kinase activity
GO:0005524--F:ATP binding
GO:0016779--F:nucleotidyltransferase activity
GO:0004781--F:sulfate adenylyltransferase (ATP) activity
GO:0050428--P:3'-phosphoadenosine 5'-phosphosulfate biosynthetic process
GO:0050427--P:3'-phosphoadenosine 5'-phosphosulfate metabolic process
GO:0005975--P:carbohydrate metabolic process
GO:0030203--P:glycosaminoglycan metabolic process
GO:0001501--P:skeletal system development
GO:0044281--P:small molecule metabolic process
GO:0000103--P:sulfate assimilation
GO:0006805--P:xenobiotic metabolic process